Vesicle-associated double-stranded ribonucleic acid genetic elements in Agaricus bisporus

C. Peter Romaine, Beth Schlagnhaufer, Michael M. Goodin

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Double-stranded ribonucleic acids (dsRNAs) were isolated from fruit bodies of commercial strains of the cultivated mushroom (Agaricus bisporus) by poly-ethylene glycol-NaCl precipitation, differential centrifugation, rate-zonal centrifugation in sucrose, and equilibrium centrifugation in cesium sulphate. In all seven of the mushroom isolates examined, three dsRNAs were identified: two major dsRNA segments of >13.1-kb (L-RNA) and 2.4-kb (S-RNA) and a minor segment of 5.2-kb (M-RNA). L-, M-, and S-RNAs co-purified with spherical fungal vesicles measuring approximately 75 nm in diameter. The three dsRNAs were intimately associated with the vesicles as suggested by their lower buoyant density in cesium sulphate (1.27 g/cc) compared to that of phenol-extracted dsRNAs (1.42 g/cc) and by their resistance to hydrolysis by ribonuclease at low ionic strength. Using a variety of conditions during purification, no virus-like particles were found to be associated with the dsRNAs. In Northern analysis, L-, M-, and S-RNAs failed to cross-hybridize with the genomic dsRNAs of La France isometric virus. We report here the first description of non-encapsidated, vesicle-associated, dsRNA genetic elements in the common cultivated mushroom.

Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalCurrent Genetics
Volume25
Issue number2
DOIs
StatePublished - Feb 1994

Keywords

  • Agaricus bisporus
  • Fungal vesicles
  • dsRNA
  • dsRNA genetic elements

ASJC Scopus subject areas

  • Genetics

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