Vestibular and cochlear efferent neurons in the monkey identified by immunocytochemical methods

M. B. Carpenter; L. Chang; A. B. Pereira; L. B. Hersh; G. Bruce; J. Y. Wu

doi:10.1016/0006-8993(87)90387-8

Vestibular and cochlear efferent neurons in the monkey identified by immunocytochemical methods

M. B. Carpenter, L. Chang, A. B. Pereira, L. B. Hersh, G. Bruce, J. Y. Wu

Research output: Contribution to journal › Article › peer-review

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Abstract

Attempts were made to identify vestibular (VEN) and cochlear (CEN) efferent neurons in the squirrel monkey using retrograde transport of horseradish peroxidase (HRP) and immunocytochemical methods. HRP implants in the ampulla of the lateral semicircular duct retrogradely labeled cells of VEN bilaterally and some cells of CEN. VEN located lateral to the rostral part of the abducens nucleus formed a compact collection of cells, all of which were immunoreactive only to antisera for choline acetyltransferase (ChAT). CEN, identified by immunoreactivity to ChAT were located at the hilus of the lateral superior olive (LSO), along the lateral border of the LSO and sparsely near lateral parts of the ventral trapezoid nucleus (VTN). A small number of cells and fibers near the border of the VTN and lateral to the LSO were immunoreactive for leucine enkephalin (L-ENK). Fibers immunoreactive for L-ENK also were identified in the hilus of the LSO. No cells of the superior olivary complex were immunoreactive for antisera to ChAT, L-ENK, substance P, γ-aminobutyric acid or glutamic acid decarboxylase. Cells of VEN and CEN can be identified by their immunoreactivity to ChAT, and some cells and fibers of CEN also contain L-ENK.

Original language	English
Pages (from-to)	275-280
Number of pages	6
Journal	Brain Research
Volume	408
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(87)90387-8
State	Published - Apr 7 1987

Bibliographical note

Funding Information:
This work was supportedb y research Grant C07005 from the Departmenot f Defense, Uniformed ServicesU niversityo f the Health Sciences (MBC) and Grant AG05893f romthe NationaIln sti-tuteso f Health (LBH). The opinionsa ndassertions containehde reina re the privateo nesof the authors and are not to be construedas official or reflecting the view of the Departmenotf Defenseo r the Uniformed ServicesU niversityo f the Health Sciences. The experimenrtes portedh ereinw erec onducteadc - cordingt o the principless et forth in the 'Guide for the Care and Use of LaboratoryA nimals',In stitute of LaboratorAy nimalR esourcesN, ationaRl esearch CouncilN IH Publicatio8n0 -23.

Funding

This work was supportedb y research Grant C07005 from the Departmenot f Defense, Uniformed ServicesU niversityo f the Health Sciences (MBC) and Grant AG05893f romthe NationaIln sti-tuteso f Health (LBH). The opinionsa ndassertions containehde reina re the privateo nesof the authors and are not to be construedas official or reflecting the view of the Departmenotf Defenseo r the Uniformed ServicesU niversityo f the Health Sciences. The experimenrtes portedh ereinw erec onducteadc - cordingt o the principless et forth in the 'Guide for the Care and Use of LaboratoryA nimals',In stitute of LaboratorAy nimalR esourcesN, ationaRl esearch CouncilN IH Publicatio8n0 -23.

Funders	Funder number
Departmenot f Defense
MBC
Uniformed ServicesU niversityo f the Health Sciences
National Institute on Aging	R01AG005893

Keywords

Abducens nucleus
Choline acetyltransferase
Leucine enkephalin
Olivocochlear bundle
Periolivary nucleus
Superior olivary complex
Vestibular efferent neuron

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(87)90387-8

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@article{2d01bdadc0df4242b0f0e6bdc18a069e,

title = "Vestibular and cochlear efferent neurons in the monkey identified by immunocytochemical methods",

abstract = "Attempts were made to identify vestibular (VEN) and cochlear (CEN) efferent neurons in the squirrel monkey using retrograde transport of horseradish peroxidase (HRP) and immunocytochemical methods. HRP implants in the ampulla of the lateral semicircular duct retrogradely labeled cells of VEN bilaterally and some cells of CEN. VEN located lateral to the rostral part of the abducens nucleus formed a compact collection of cells, all of which were immunoreactive only to antisera for choline acetyltransferase (ChAT). CEN, identified by immunoreactivity to ChAT were located at the hilus of the lateral superior olive (LSO), along the lateral border of the LSO and sparsely near lateral parts of the ventral trapezoid nucleus (VTN). A small number of cells and fibers near the border of the VTN and lateral to the LSO were immunoreactive for leucine enkephalin (L-ENK). Fibers immunoreactive for L-ENK also were identified in the hilus of the LSO. No cells of the superior olivary complex were immunoreactive for antisera to ChAT, L-ENK, substance P, γ-aminobutyric acid or glutamic acid decarboxylase. Cells of VEN and CEN can be identified by their immunoreactivity to ChAT, and some cells and fibers of CEN also contain L-ENK.",

keywords = "Abducens nucleus, Choline acetyltransferase, Leucine enkephalin, Olivocochlear bundle, Periolivary nucleus, Superior olivary complex, Vestibular efferent neuron",

author = "Carpenter, {M. B.} and L. Chang and Pereira, {A. B.} and Hersh, {L. B.} and G. Bruce and Wu, {J. Y.}",

note = "Funding Information: This work was supportedb y research Grant C07005 from the Departmenot f Defense, Uniformed ServicesU niversityo f the Health Sciences (MBC) and Grant AG05893f romthe NationaIln sti-tuteso f Health (LBH). The opinionsa ndassertions containehde reina re the privateo nesof the authors and are not to be construedas official or reflecting the view of the Departmenotf Defenseo r the Uniformed ServicesU niversityo f the Health Sciences. The experimenrtes portedh ereinw erec onducteadc - cordingt o the principless et forth in the 'Guide for the Care and Use of LaboratoryA nimals',In stitute of LaboratorAy nimalR esourcesN, ationaRl esearch CouncilN IH Publicatio8n0 -23.",

year = "1987",

month = apr,

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doi = "10.1016/0006-8993(87)90387-8",

language = "English",

volume = "408",

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T1 - Vestibular and cochlear efferent neurons in the monkey identified by immunocytochemical methods

AU - Carpenter, M. B.

AU - Chang, L.

AU - Pereira, A. B.

AU - Hersh, L. B.

AU - Bruce, G.

AU - Wu, J. Y.

N1 - Funding Information: This work was supportedb y research Grant C07005 from the Departmenot f Defense, Uniformed ServicesU niversityo f the Health Sciences (MBC) and Grant AG05893f romthe NationaIln sti-tuteso f Health (LBH). The opinionsa ndassertions containehde reina re the privateo nesof the authors and are not to be construedas official or reflecting the view of the Departmenotf Defenseo r the Uniformed ServicesU niversityo f the Health Sciences. The experimenrtes portedh ereinw erec onducteadc - cordingt o the principless et forth in the 'Guide for the Care and Use of LaboratoryA nimals',In stitute of LaboratorAy nimalR esourcesN, ationaRl esearch CouncilN IH Publicatio8n0 -23.

PY - 1987/4/7

Y1 - 1987/4/7

N2 - Attempts were made to identify vestibular (VEN) and cochlear (CEN) efferent neurons in the squirrel monkey using retrograde transport of horseradish peroxidase (HRP) and immunocytochemical methods. HRP implants in the ampulla of the lateral semicircular duct retrogradely labeled cells of VEN bilaterally and some cells of CEN. VEN located lateral to the rostral part of the abducens nucleus formed a compact collection of cells, all of which were immunoreactive only to antisera for choline acetyltransferase (ChAT). CEN, identified by immunoreactivity to ChAT were located at the hilus of the lateral superior olive (LSO), along the lateral border of the LSO and sparsely near lateral parts of the ventral trapezoid nucleus (VTN). A small number of cells and fibers near the border of the VTN and lateral to the LSO were immunoreactive for leucine enkephalin (L-ENK). Fibers immunoreactive for L-ENK also were identified in the hilus of the LSO. No cells of the superior olivary complex were immunoreactive for antisera to ChAT, L-ENK, substance P, γ-aminobutyric acid or glutamic acid decarboxylase. Cells of VEN and CEN can be identified by their immunoreactivity to ChAT, and some cells and fibers of CEN also contain L-ENK.

AB - Attempts were made to identify vestibular (VEN) and cochlear (CEN) efferent neurons in the squirrel monkey using retrograde transport of horseradish peroxidase (HRP) and immunocytochemical methods. HRP implants in the ampulla of the lateral semicircular duct retrogradely labeled cells of VEN bilaterally and some cells of CEN. VEN located lateral to the rostral part of the abducens nucleus formed a compact collection of cells, all of which were immunoreactive only to antisera for choline acetyltransferase (ChAT). CEN, identified by immunoreactivity to ChAT were located at the hilus of the lateral superior olive (LSO), along the lateral border of the LSO and sparsely near lateral parts of the ventral trapezoid nucleus (VTN). A small number of cells and fibers near the border of the VTN and lateral to the LSO were immunoreactive for leucine enkephalin (L-ENK). Fibers immunoreactive for L-ENK also were identified in the hilus of the LSO. No cells of the superior olivary complex were immunoreactive for antisera to ChAT, L-ENK, substance P, γ-aminobutyric acid or glutamic acid decarboxylase. Cells of VEN and CEN can be identified by their immunoreactivity to ChAT, and some cells and fibers of CEN also contain L-ENK.

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KW - Choline acetyltransferase

KW - Leucine enkephalin

KW - Olivocochlear bundle

KW - Periolivary nucleus

KW - Superior olivary complex

KW - Vestibular efferent neuron

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M3 - Article

C2 - 2439167

AN - SCOPUS:0023227957

SN - 0006-8993

VL - 408

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JF - Brain Research

IS - 1-2

ER -

Vestibular and cochlear efferent neurons in the monkey identified by immunocytochemical methods

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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