TY - CHAP
T1 - Virus-induced gene silencing in soybean
AU - Kachroo, Aardra
AU - Ghabrial, Said
PY - 2012
Y1 - 2012
N2 - Understanding the biology of a system requires the association of gene functions to phenotypes and vice versa. Although a large number of resources and genomic tools are available for studies in the crop plant soybean, investigations related to gene function have been lacking. This is largely due to the fact that rapid functional genomics tools have been unavailable for application in soybean. This constraint was recently alleviated when a novel bean pod mottle virus (BPMV)-based system for virus-induced gene silencing in soybean was developed. This methodology exploits the plant's ability to silence virus-encoded sequences as a defense mechanism. Plants infected with recombinant viruses carrying sequences that are identical/near-identical to the plant's own genes suppress the expression of the target endogenous gene(s). Phenotypes associated with suppression of target sequence enable the assignment of functions to specific genes. The strategy involves inserting short sequences of target soybean genes into the viral genome to generate recombinant vectors. Soybean plants are then inoculated with the recombinant vectors, assessed for BPMV propagation and silencing of the target genes. Plants silenced for specific targets can then be analyzed for various different traits depending upon the known/predicted functions of the target genes. Using this strategy, we have identified genes participating in basal, resistance gene-mediated, and systemic immunity in soybean.
AB - Understanding the biology of a system requires the association of gene functions to phenotypes and vice versa. Although a large number of resources and genomic tools are available for studies in the crop plant soybean, investigations related to gene function have been lacking. This is largely due to the fact that rapid functional genomics tools have been unavailable for application in soybean. This constraint was recently alleviated when a novel bean pod mottle virus (BPMV)-based system for virus-induced gene silencing in soybean was developed. This methodology exploits the plant's ability to silence virus-encoded sequences as a defense mechanism. Plants infected with recombinant viruses carrying sequences that are identical/near-identical to the plant's own genes suppress the expression of the target endogenous gene(s). Phenotypes associated with suppression of target sequence enable the assignment of functions to specific genes. The strategy involves inserting short sequences of target soybean genes into the viral genome to generate recombinant vectors. Soybean plants are then inoculated with the recombinant vectors, assessed for BPMV propagation and silencing of the target genes. Plants silenced for specific targets can then be analyzed for various different traits depending upon the known/predicted functions of the target genes. Using this strategy, we have identified genes participating in basal, resistance gene-mediated, and systemic immunity in soybean.
KW - Bean pod-mottle virus
KW - Defense signaling
KW - Functional genomics
KW - Soybean
KW - Virus-induced gene silencing
UR - http://www.scopus.com/inward/record.url?scp=84934444226&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84934444226&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-882-5_19
DO - 10.1007/978-1-61779-882-5_19
M3 - Chapter
C2 - 22678587
AN - SCOPUS:84934444226
SN - 9781617798818
T3 - Methods in Molecular Biology
SP - 287
EP - 297
BT - Antiviral Resistance in Plants
ER -