Disturbances in arterial wall elastin metabolism appear to be important factors in atherosclerosis development. To evaluate this hypothesis, elastase-like activity was determined in cultured endothelial cells and their surrounding media after exposure to tumor necrosis factor-α (TNF), cholestan-3β,5α,6β-triol (Triol) and linoleic acid (18:2). Significant increases in elastase-like activity both in the cells and in the media were observed when subconfluent endothelial cells were treated with 12 μM Triol, 500 U TNF/ml, or 90 μM 18:2, for 72 h in the presence of 5% calf serum. Even higher activities were measured when endothelial cells were seeded directly into media enriched with 18:2, TNF or Triol and treated for 72 h. Vitamin E supplementation (25 μM) attenuated elastase-like activity in cells and media, independent of treatment. These results suggest that elastase-like enzyme induction in endothelial cells may be involved in cellular pertubations induced by certain lipids and cytokines. Vitamin E may provide a protective function by preventing the induction of elastolytic enzymes. This may have implications in elastin metabolism and atherosclerosis.
|Number of pages||11|
|Journal||Clinica Chimica Acta|
|State||Published - Jun 16 1993|
Bibliographical noteFunding Information:
Supported in part by grant HL-36552 from the National Heart, Lung, and Blood Institute, National Institutes of Health; grants from the National Live Stock and Meat Board and National Dairy Councii and the Kentucky Agricultural Experimental Station (article number 92-9-202). We apl:reciate the generosity of Genetech, !nc (San Francisco, CA) in supplying recombinant human TNF-ot. We gratefully thank Natalie Goh for her technical assistance.
- Elastase-like activity
- Endothelial cells
- Linoleic acid
- Tumor necrosis factor-α
- Vitamin E
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical