VvBBX44 and VvMYBA1 form a regulatory feedback loop to balance anthocyanin biosynthesis in grape

Wenwen Liu; Huayuan Mu; Ling Yuan; Yang Li; Yuting Li; Shenchang Li; Chong Ren; Wei Duan; Peige Fan; Zhanwu Dai; Yongfeng Zhou; Zhenchang Liang; Shaohua Li; Lijun Wang

doi:10.1093/hr/uhad176

VvBBX44 and VvMYBA1 form a regulatory feedback loop to balance anthocyanin biosynthesis in grape

Wenwen Liu, Huayuan Mu, Ling Yuan, Yang Li, Yuting Li, Shenchang Li, Chong Ren, Wei Duan, Peige Fan, Zhanwu Dai, Yongfeng Zhou, Zhenchang Liang, Shaohua Li, Lijun Wang

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Anthocyanins are essential for the quality of perennial horticultural crops, such as grapes. In grapes, ELONGATED HYPOCOTYL 5 (HY5) and MYBA1 are two critical transcription factors that regulate anthocyanin biosynthesis. Our previous work has shown that Vitis vinifera B-box protein 44 (VvBBX44) inhibits anthocyanin synthesis and represses VvHY5 expression in grape calli. However, the regulatory mechanism underlying this regulation was unclear. In this study, we found that loss of VvBBX44 function resulted in increased anthocyanin accumulation in grapevine callus. VvBBX44 directly represses VvMYBA1, which activates VvBBX44. VvMYBA1, but not VvBBX44, directly modulates the expression of grape UDP flavonoid 3-O-glucosyltransferase (VvUFGT). We demonstrated that VvBBX44 represses the transcriptional activation of VvUFGT and VvBBX44 induced by VvMYBA1. However, VvBBX44 and VvMYBA1 did not physically interact in yeast. The application of exogenous anthocyanin stimulated VvBBX44 expression in grapevine suspension cells and tobacco leaves. These findings suggest that VvBBX44 and VvMYBA1 form a transcriptional feedback loop to prevent overaccumulation of anthocyanin and reduce metabolic costs. Our work sheds light on the complex regulatory network that controls anthocyanin biosynthesis in grapevine.

Original language	English
Article number	uhad176
Journal	Horticulture Research
Volume	10
Issue number	10
DOIs	https://doi.org/10.1093/hr/uhad176
State	Published - Oct 1 2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press on behalf of Nanjing Agricultural University.

Funding

We thank professor Yu-Jin Hao (College of Horticulture Science and Engineering, Shandong Agricultural University) for providing the plasmid for the EMSA experiment. All data generated and analyzed in this study are shown in the article or attached as supplementary data. All the materials used in the study are available upon reasonable request from the corresponding author. This work was supported by the National Natural Science Foundation of China (Grant no. U21A20227),and the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant no. XDA23080602). Research conducted as part of the LIA INNOGRAPE International Associated Laboratory. Author contributions

Funders	Funder number
National Natural Science Foundation of China (NSFC)	U21A20227
National Natural Science Foundation of China (NSFC)
Chinese Academy of Sciences	XDA23080602
Chinese Academy of Sciences

ASJC Scopus subject areas

Biotechnology
Biochemistry
Genetics
Plant Science
Horticulture

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10.1093/hr/uhad176

Cite this

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title = "VvBBX44 and VvMYBA1 form a regulatory feedback loop to balance anthocyanin biosynthesis in grape",

abstract = "Anthocyanins are essential for the quality of perennial horticultural crops, such as grapes. In grapes, ELONGATED HYPOCOTYL 5 (HY5) and MYBA1 are two critical transcription factors that regulate anthocyanin biosynthesis. Our previous work has shown that Vitis vinifera B-box protein 44 (VvBBX44) inhibits anthocyanin synthesis and represses VvHY5 expression in grape calli. However, the regulatory mechanism underlying this regulation was unclear. In this study, we found that loss of VvBBX44 function resulted in increased anthocyanin accumulation in grapevine callus. VvBBX44 directly represses VvMYBA1, which activates VvBBX44. VvMYBA1, but not VvBBX44, directly modulates the expression of grape UDP flavonoid 3-O-glucosyltransferase (VvUFGT). We demonstrated that VvBBX44 represses the transcriptional activation of VvUFGT and VvBBX44 induced by VvMYBA1. However, VvBBX44 and VvMYBA1 did not physically interact in yeast. The application of exogenous anthocyanin stimulated VvBBX44 expression in grapevine suspension cells and tobacco leaves. These findings suggest that VvBBX44 and VvMYBA1 form a transcriptional feedback loop to prevent overaccumulation of anthocyanin and reduce metabolic costs. Our work sheds light on the complex regulatory network that controls anthocyanin biosynthesis in grapevine.",

author = "Wenwen Liu and Huayuan Mu and Ling Yuan and Yang Li and Yuting Li and Shenchang Li and Chong Ren and Wei Duan and Peige Fan and Zhanwu Dai and Yongfeng Zhou and Zhenchang Liang and Shaohua Li and Lijun Wang",

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AU - Mu, Huayuan

AU - Yuan, Ling

AU - Li, Yang

AU - Li, Yuting

AU - Li, Shenchang

AU - Ren, Chong

AU - Duan, Wei

AU - Fan, Peige

AU - Dai, Zhanwu

AU - Zhou, Yongfeng

AU - Liang, Zhenchang

AU - Li, Shaohua

AU - Wang, Lijun

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AB - Anthocyanins are essential for the quality of perennial horticultural crops, such as grapes. In grapes, ELONGATED HYPOCOTYL 5 (HY5) and MYBA1 are two critical transcription factors that regulate anthocyanin biosynthesis. Our previous work has shown that Vitis vinifera B-box protein 44 (VvBBX44) inhibits anthocyanin synthesis and represses VvHY5 expression in grape calli. However, the regulatory mechanism underlying this regulation was unclear. In this study, we found that loss of VvBBX44 function resulted in increased anthocyanin accumulation in grapevine callus. VvBBX44 directly represses VvMYBA1, which activates VvBBX44. VvMYBA1, but not VvBBX44, directly modulates the expression of grape UDP flavonoid 3-O-glucosyltransferase (VvUFGT). We demonstrated that VvBBX44 represses the transcriptional activation of VvUFGT and VvBBX44 induced by VvMYBA1. However, VvBBX44 and VvMYBA1 did not physically interact in yeast. The application of exogenous anthocyanin stimulated VvBBX44 expression in grapevine suspension cells and tobacco leaves. These findings suggest that VvBBX44 and VvMYBA1 form a transcriptional feedback loop to prevent overaccumulation of anthocyanin and reduce metabolic costs. Our work sheds light on the complex regulatory network that controls anthocyanin biosynthesis in grapevine.

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VvBBX44 and VvMYBA1 form a regulatory feedback loop to balance anthocyanin biosynthesis in grape

Abstract

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