TY - JOUR
T1 - Werner syndrome protein
T2 - Biochemical properties and functional interactions
AU - Bohr, V. A.
AU - Cooper, M.
AU - Orren, D.
AU - Machwe, A.
AU - Piotrowski, J.
AU - Sommers, J.
AU - Karmakar, P.
AU - Brosh, R.
PY - 2000
Y1 - 2000
N2 - Werner syndrome is a premature aging syndrome displaying numerous signs and symptoms found in normal aging. The disease is associated with a mutation in the WRN gene. We have purified the Werner protein (WRN) and studied its biochemical activities and its protein interactions. WRN is a helicase and an exonuclease and also has an associated ATPase activity. WRN interacts physically and functionally with replication protein A (RPA), which stimulates its helicase activity. We have studied the WRN exonuclease activity and found that it can be blocked by certain DNA lesions and not by others. Thus, while WRN does not bind to DNA damage, it may have properties that allow it to sense the presence of damage in DNA. More recently we have found other protein interactions that involve physical and functional interactions with WRN, which could suggest a role for WRN in DNA repair. (C) 2000 Elsevier Science Inc.
AB - Werner syndrome is a premature aging syndrome displaying numerous signs and symptoms found in normal aging. The disease is associated with a mutation in the WRN gene. We have purified the Werner protein (WRN) and studied its biochemical activities and its protein interactions. WRN is a helicase and an exonuclease and also has an associated ATPase activity. WRN interacts physically and functionally with replication protein A (RPA), which stimulates its helicase activity. We have studied the WRN exonuclease activity and found that it can be blocked by certain DNA lesions and not by others. Thus, while WRN does not bind to DNA damage, it may have properties that allow it to sense the presence of damage in DNA. More recently we have found other protein interactions that involve physical and functional interactions with WRN, which could suggest a role for WRN in DNA repair. (C) 2000 Elsevier Science Inc.
KW - DNA repair
KW - Replication protein
KW - Werner syndrome protein
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U2 - 10.1016/S0531-5565(00)00145-5
DO - 10.1016/S0531-5565(00)00145-5
M3 - Article
C2 - 11053659
AN - SCOPUS:0033792458
SN - 0531-5565
VL - 35
SP - 695
EP - 702
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 6-7
ER -