Wide-ranging transcriptome remodelling mediated by alternative polyadenylation in response to abiotic stresses in Sorghum

Manohar Chakrabarti, Laura de Lorenzo, Salah E. Abdel-Ghany, Anireddy S.N. Reddy, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Alternative polyadenylation (APA) regulates diverse developmental and physiological processes through its effects on gene expression, mRNA stability, translatability, and transport. Sorghum is a major cereal crop in the world and, despite its importance, not much is known about the role of post-transcriptional regulation in mediating responses to abiotic stresses in Sorghum. A genome-wide APA analysis unveiled widespread occurrence of APA in Sorghum in response to drought, heat, and salt stress. Abiotic stress treatments incited changes in poly(A) site choice in a large number of genes. Interestingly, abiotic stresses led to the re-directing of transcriptional output into non-productive pathways defined by the class of poly(A) site utilized. This result revealed APA to be part of a larger global response of Sorghum to abiotic stresses that involves the re-direction of transcriptional output into non-productive transcriptional and translational pathways. Large numbers of stress-inducible poly(A) sites could not be linked with known, annotated genes, suggestive of the existence of numerous unidentified genes whose expression is strongly regulated by abiotic stresses. Furthermore, we uncovered a novel stress-specific cis-element in intronic poly(A) sites used in drought- and heat-stressed plants that might play an important role in non-canonical poly(A) site choice in response to abiotic stresses.

Original languageEnglish
Pages (from-to)916-930
Number of pages15
JournalPlant Journal
Volume102
Issue number5
DOIs
StatePublished - Jun 1 2020

Bibliographical note

Funding Information:
This research was supported by the National Science Foundation (Award MCB‐1243849) (AGH), the USDA National Institute of Food and Agriculture [Hatch project KY006089] (AGH), and the DOE Office of Science, Office of Biological and Environmental Research (BER) (Grant No. DE‐SC0010733) (ASNR).

Funding Information:
This research was supported by the National Science Foundation (Award MCB-1243849) (AGH), the USDA National Institute of Food and Agriculture [Hatch project KY006089] (AGH), and the DOE Office of Science, Office of Biological and Environmental Research (BER) (Grant No. DE-SC0010733) (ASNR).

Publisher Copyright:
© 2020 The Authors The Plant Journal © 2020 John Wiley & Sons Ltd

Keywords

  • APA
  • Sorghum
  • abiotic stress
  • alternative polyadenylation
  • drought
  • heat
  • salinity

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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