XRF and XAFS analysis of electrophoretically isolated nondenatured proteins

K. M. Kemner, S. D. Kelly, E. J. O'Loughlin, T. Khare, L. A. Moe, B. G. Fox, M. I. Donnelly, Y. Londer, M. Schiffer, C. S. Giometti

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations


Microbial metal reduction is relevant for developing bioremediation strategies for contaminated sites containing toxic metal compounds, but the molecular mechanisms involved and the regulation of those mechanisms are poorly understood. Metal reduction capability is microbe specific, yet the involvement of metalloproteins in the transport of electrons from electron donor molecules to electron acceptor molecules (i.e., to the metals to be reduced) is common to all metal-reducing organisms. A thorough understanding of the mechanisms involved in metal reduction by microbes, with a focus on their metalloproteins, will provide valuable information about the metal reduction capability of the organisms and could provide general insight into the mechanisms of metalloprotein expression and regulation in other microbial systems as well. We are developing methods for the efficient separation, detection, and quantification of metalloproteins. Electrophoretic methods for protein separation have been combined with x-ray fluorescence mapping and XAFS to determine the abundance and local chemical environment of iron within a cytochrome.

Original languageEnglish
Pages (from-to)940-942
Number of pages3
JournalPhysica Scripta T
StatePublished - 2005
Event12th X-ray Absorption Fine Structure International Conference, XAFS12 - Malmo, Sweden
Duration: Jun 23 2003Jun 27 2003

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics
  • Mathematical Physics


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