RII Track-2 FEC: Membrane Purification Platform for Continuous Biomanufacturing of Virus Particles in Arkansas and Beyond

Scope of Work RII Track-2 FEC: Membrane Purification Platform for Continuous Biomanufacturing of Virus Particles in Arkansas and Beyond Subcontract to University of Kentucky from University of Arkansas The University of Kentucky collaborators will produce pseudotypep virus particles and virus-like particles (VLPs) for testing of separation strategies for viral vectors. Synthesis and functionalization strategies for microfiltration, ultrafiltration and nanofiltration membranes comprising organic, inorganic, and composites will be developed for viral vector separations, with a particular focus on opportunities to separate full/empty capsids. Besides advancing overall virus separation strategies, the ability to create higher concentration full capsids with minimal empty capsid impurity will advance health related applications immensely. As more complex viral vector systems become available from the collaboration with the University of Arkansas, the separation and purification of these systems will be examined using the membranes developed by the University of Kentucky.