Supplement: Lafora Epilepsy: Basic Mechanisms to Therapy

This is an Administrative Supplement request in response to PA-18-591 for “Administrative Supplements to Existing NIH Grants and Cooperative Agreements.” The requested funds are for two pieces of equipment that will be utilized by P01NS097197 and R01NS07899 to complete nonoverlapping experiments that were proposed in these applications. The pieces of equipment are 1) a capillary electrophoresis system to define the physiochemical properties of Lafora bodies and glycogen, and 2) a microwave fixation system to quickly deactivate brain enzymes and freeze glycogen and metabolites for analysis. The request is being made due to unforeseen costs and delays concerning capillary electrophoresis analysis of glycogen, and evolving methodologies that were not available at the time of submission regarding necessary brain fixation for metabolite analysis. The research objectives of this supplement are within the original scope of the peer reviewed proposal and approved project. If supported, these funds will increase the overall impact of the originally approved aims.