α-Naphthaleneacetic acid induces the expression of seedling lipoxygenases in cultured immature zygotic soybean embryo cotyledons

C. Wang; K. P.C. Croft; D. F. Hildebrand

doi:10.1007/s002990000258

α-Naphthaleneacetic acid induces the expression of seedling lipoxygenases in cultured immature zygotic soybean embryo cotyledons

C. Wang
, K. P.C. Croft
, D. F. Hildebrand

Plant and Soil Sciences

Producción científica: Article › revisión exhaustiva

2 Citas (Scopus)

Resumen

Auxin [α-naphthaleneacetic acid (NAA) or indole-3-acetic acid] can induce the expression of lipoxygenases (LOXs) in cultured immature zygotic embryo cotyledons of soybean [Glycine max. (L.) Merr]. These auxin-induced LOXs are different from those normally expressed in seeds but have the same isoelectric points (pI) as those found in seedlings. The pIs of the two seedling LOXs were determined to be 5.09 and 5.23. One of the auxin-induced LOXs has the same pI (5.09) and molecular mass (94 kDa) as seedling LOX4. The partial amino acid sequences from the purified NAA-induced pI-5.09 LOX are identical to those of LOX4. RNA protection assays showed that NAA induces the expression of LOX4 and LOX5 mRNAs in cultured embryo cotyledons where they are not normally expressed. Soybean genotypes with a polymorphic variant of LOX4 in hypocotyls showed the same variation as NAA-induced LOXs in the embryo cotyledons. These results demonstrate that the NAA-induced pI-5.09 LOX is seedling LOX4 and also suggest that auxin might be directly or indirectly involved in seedling LOX expression during seed germination.

Idioma original	English
Páginas (desde-hasta)	85-91
Número de páginas	7
Publicación	Plant Cell Reports
Volumen	20
N.º	1
DOI	https://doi.org/10.1007/s002990000258
Estado	Published - 2001

Nota bibliográfica

Funding Information:
Acknowledgements We thank Ms Yuan Sun for excellent assistance in the culture of soybean cotyledons, Dr Carol Beach for the amino acid sequence analysis, and Dr Wennuan Liu for help with the culture of soybean cotyledons. We also thank Dr J. Polacco for LOX4 and LOX5 cDNAs and Drs K. Kitamura and N. Nielsen for the soybean seed LOX mutants. This work was supported by USDA grant no. 9701487, the THRI and the Kentucky Agricultural Experiment Station and published as paper no. 96-06-065.

Financiación

Acknowledgements We thank Ms Yuan Sun for excellent assistance in the culture of soybean cotyledons, Dr Carol Beach for the amino acid sequence analysis, and Dr Wennuan Liu for help with the culture of soybean cotyledons. We also thank Dr J. Polacco for LOX4 and LOX5 cDNAs and Drs K. Kitamura and N. Nielsen for the soybean seed LOX mutants. This work was supported by USDA grant no. 9701487, the THRI and the Kentucky Agricultural Experiment Station and published as paper no. 96-06-065.

Financiadores	Número del financiador
THRI
U.S. Department of Agriculture	9701487
Kentucky Agricultural Experiment Station	96-06-065

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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@article{322f7a4dbf164164a43786c7ca48b5aa,

title = "α-Naphthaleneacetic acid induces the expression of seedling lipoxygenases in cultured immature zygotic soybean embryo cotyledons",

abstract = "Auxin [α-naphthaleneacetic acid (NAA) or indole-3-acetic acid] can induce the expression of lipoxygenases (LOXs) in cultured immature zygotic embryo cotyledons of soybean [Glycine max. (L.) Merr]. These auxin-induced LOXs are different from those normally expressed in seeds but have the same isoelectric points (pI) as those found in seedlings. The pIs of the two seedling LOXs were determined to be 5.09 and 5.23. One of the auxin-induced LOXs has the same pI (5.09) and molecular mass (94 kDa) as seedling LOX4. The partial amino acid sequences from the purified NAA-induced pI-5.09 LOX are identical to those of LOX4. RNA protection assays showed that NAA induces the expression of LOX4 and LOX5 mRNAs in cultured embryo cotyledons where they are not normally expressed. Soybean genotypes with a polymorphic variant of LOX4 in hypocotyls showed the same variation as NAA-induced LOXs in the embryo cotyledons. These results demonstrate that the NAA-induced pI-5.09 LOX is seedling LOX4 and also suggest that auxin might be directly or indirectly involved in seedling LOX expression during seed germination.",

keywords = "Auxin, Cotyledon, Glycine max, Immature zygotic embryo, Lipoxygenase",

author = "C. Wang and Croft, \{K. P.C.\} and Hildebrand, \{D. F.\}",

note = "Funding Information: Acknowledgements We thank Ms Yuan Sun for excellent assistance in the culture of soybean cotyledons, Dr Carol Beach for the amino acid sequence analysis, and Dr Wennuan Liu for help with the culture of soybean cotyledons. We also thank Dr J. Polacco for LOX4 and LOX5 cDNAs and Drs K. Kitamura and N. Nielsen for the soybean seed LOX mutants. This work was supported by USDA grant no. 9701487, the THRI and the Kentucky Agricultural Experiment Station and published as paper no. 96-06-065.",

year = "2001",

doi = "10.1007/s002990000258",

language = "English",

volume = "20",

pages = "85--91",

number = "1",

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TY - JOUR

T1 - α-Naphthaleneacetic acid induces the expression of seedling lipoxygenases in cultured immature zygotic soybean embryo cotyledons

AU - Wang, C.

AU - Croft, K. P.C.

AU - Hildebrand, D. F.

N1 - Funding Information: Acknowledgements We thank Ms Yuan Sun for excellent assistance in the culture of soybean cotyledons, Dr Carol Beach for the amino acid sequence analysis, and Dr Wennuan Liu for help with the culture of soybean cotyledons. We also thank Dr J. Polacco for LOX4 and LOX5 cDNAs and Drs K. Kitamura and N. Nielsen for the soybean seed LOX mutants. This work was supported by USDA grant no. 9701487, the THRI and the Kentucky Agricultural Experiment Station and published as paper no. 96-06-065.

PY - 2001

Y1 - 2001

N2 - Auxin [α-naphthaleneacetic acid (NAA) or indole-3-acetic acid] can induce the expression of lipoxygenases (LOXs) in cultured immature zygotic embryo cotyledons of soybean [Glycine max. (L.) Merr]. These auxin-induced LOXs are different from those normally expressed in seeds but have the same isoelectric points (pI) as those found in seedlings. The pIs of the two seedling LOXs were determined to be 5.09 and 5.23. One of the auxin-induced LOXs has the same pI (5.09) and molecular mass (94 kDa) as seedling LOX4. The partial amino acid sequences from the purified NAA-induced pI-5.09 LOX are identical to those of LOX4. RNA protection assays showed that NAA induces the expression of LOX4 and LOX5 mRNAs in cultured embryo cotyledons where they are not normally expressed. Soybean genotypes with a polymorphic variant of LOX4 in hypocotyls showed the same variation as NAA-induced LOXs in the embryo cotyledons. These results demonstrate that the NAA-induced pI-5.09 LOX is seedling LOX4 and also suggest that auxin might be directly or indirectly involved in seedling LOX expression during seed germination.

AB - Auxin [α-naphthaleneacetic acid (NAA) or indole-3-acetic acid] can induce the expression of lipoxygenases (LOXs) in cultured immature zygotic embryo cotyledons of soybean [Glycine max. (L.) Merr]. These auxin-induced LOXs are different from those normally expressed in seeds but have the same isoelectric points (pI) as those found in seedlings. The pIs of the two seedling LOXs were determined to be 5.09 and 5.23. One of the auxin-induced LOXs has the same pI (5.09) and molecular mass (94 kDa) as seedling LOX4. The partial amino acid sequences from the purified NAA-induced pI-5.09 LOX are identical to those of LOX4. RNA protection assays showed that NAA induces the expression of LOX4 and LOX5 mRNAs in cultured embryo cotyledons where they are not normally expressed. Soybean genotypes with a polymorphic variant of LOX4 in hypocotyls showed the same variation as NAA-induced LOXs in the embryo cotyledons. These results demonstrate that the NAA-induced pI-5.09 LOX is seedling LOX4 and also suggest that auxin might be directly or indirectly involved in seedling LOX expression during seed germination.

KW - Auxin

KW - Cotyledon

KW - Glycine max

KW - Immature zygotic embryo

KW - Lipoxygenase

UR - https://www.scopus.com/pages/publications/0035037077

UR - https://www.scopus.com/pages/publications/0035037077#tab=citedBy

U2 - 10.1007/s002990000258

DO - 10.1007/s002990000258

M3 - Article

AN - SCOPUS:0035037077

SN - 0721-7714

VL - 20

SP - 85

EP - 91

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 1

ER -

α-Naphthaleneacetic acid induces the expression of seedling lipoxygenases in cultured immature zygotic soybean embryo cotyledons

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Nota bibliográfica

Financiación

ASJC Scopus subject areas

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