β-catenin interacts with and inhibits NF-κB in human colon and breast cancer

Jiong Deng; Stephanie A. Miller; Hong Ying Wang; Weiya Xia; Yong Wen; Binhua P. Zhou; Yan Li; Shiaw Yih Lin; Mien Chie Hung

doi:10.1016/S1535-6108(02)00154-X

β-catenin interacts with and inhibits NF-κB in human colon and breast cancer

Jiong Deng
, Stephanie A. Miller
, Hong Ying Wang
, Weiya Xia
, Yong Wen
, Binhua P. Zhou
, Yan Li
, Shiaw Yih Lin
, Mien Chie Hung

Producción científica: Article › revisión exhaustiva

350 Citas (Scopus)

Resumen

β-catenin plays an important role in development and homeostasis. Deregulated β-catenin is involved in oncogenesis. In this study, we found that β-catenin can physically complex with NF-κB, resulting in a reduction of NF-κB DNA binding, transactivation activity, and target gene expression. Repressed NF-κB activity is found in human colon cancer cells in which β-catenin is activated. Importantly, activated β-catenin was found to inhibit the expression of NF-κB target genes, including Fas and TRAF1. Furthermore, a strong inverse correlation was identified between the expression levels of β-catenin and Fas in colon and breast tumor tissues, suggesting that β-catenin regulates NF-κB and its targets in vivo. Thus, β-catenin may play an important role in oncogenesis through the crossregulation of NF-κB.

Idioma original	English
Páginas (desde-hasta)	323-334
Número de páginas	12
Publicación	Cancer Cell
Volumen	2
N.º	4
DOI	https://doi.org/10.1016/S1535-6108(02)00154-X
Estado	Published - oct 2002

Nota bibliográfica

Funding Information:
This work was supported by NIH grants CA58880 and Ovarian SPORE grant P50 CA83639 (to M.-C. Hung), by a predoctoral fellowship from the US Army Breast Cancer Research Training Grant Program, Grant No. DAMD17-99-1-9264 (to Y. Wen), and Grant No. DAMD 17-02-1-0454 (to Y. Li). We would like to thank Dr. S.W. Byers for the wild type β-catenin and β-cateninS37A plasmids, Dr. H. Clevers for the dominant negative TCF4 (pcDNA3-ΔNTCF4), TOP, and FOP plasmids, and Dr. L.K. Su for the APC expression plasmid. We would also like to thank Dr. A.G. de Herreros for the GST-β-catenin, Dr. P.J. Chiao for the pBS-p65 and pBS-p105 and MEF rela −/− cells, and Dr. J. Schmid for the GFP-p65 plasmid.

Financiación

This work was supported by NIH grants CA58880 and Ovarian SPORE grant P50 CA83639 (to M.-C. Hung), by a predoctoral fellowship from the US Army Breast Cancer Research Training Grant Program, Grant No. DAMD17-99-1-9264 (to Y. Wen), and Grant No. DAMD 17-02-1-0454 (to Y. Li). We would like to thank Dr. S.W. Byers for the wild type β-catenin and β-cateninS37A plasmids, Dr. H. Clevers for the dominant negative TCF4 (pcDNA3-ΔNTCF4), TOP, and FOP plasmids, and Dr. L.K. Su for the APC expression plasmid. We would also like to thank Dr. A.G. de Herreros for the GST-β-catenin, Dr. P.J. Chiao for the pBS-p65 and pBS-p105 and MEF rela −/− cells, and Dr. J. Schmid for the GFP-p65 plasmid.

Financiadores	Número del financiador
National Institutes of Health (NIH)	DAMD17-99-1-9264, DAMD 17-02-1-0454, CA58880
National Institutes of Health (NIH)
National Childhood Cancer Registry – National Cancer Institute	P50CA083639
National Childhood Cancer Registry – National Cancer Institute

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/S1535-6108(02)00154-X

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title = "β-catenin interacts with and inhibits NF-κB in human colon and breast cancer",

abstract = "β-catenin plays an important role in development and homeostasis. Deregulated β-catenin is involved in oncogenesis. In this study, we found that β-catenin can physically complex with NF-κB, resulting in a reduction of NF-κB DNA binding, transactivation activity, and target gene expression. Repressed NF-κB activity is found in human colon cancer cells in which β-catenin is activated. Importantly, activated β-catenin was found to inhibit the expression of NF-κB target genes, including Fas and TRAF1. Furthermore, a strong inverse correlation was identified between the expression levels of β-catenin and Fas in colon and breast tumor tissues, suggesting that β-catenin regulates NF-κB and its targets in vivo. Thus, β-catenin may play an important role in oncogenesis through the crossregulation of NF-κB.",

author = "Jiong Deng and Miller, \{Stephanie A.\} and Wang, \{Hong Ying\} and Weiya Xia and Yong Wen and Zhou, \{Binhua P.\} and Yan Li and Lin, \{Shiaw Yih\} and Hung, \{Mien Chie\}",

note = "Funding Information: This work was supported by NIH grants CA58880 and Ovarian SPORE grant P50 CA83639 (to M.-C. Hung), by a predoctoral fellowship from the US Army Breast Cancer Research Training Grant Program, Grant No. DAMD17-99-1-9264 (to Y. Wen), and Grant No. DAMD 17-02-1-0454 (to Y. Li). We would like to thank Dr. S.W. Byers for the wild type β-catenin and β-cateninS37A plasmids, Dr. H. Clevers for the dominant negative TCF4 (pcDNA3-ΔNTCF4), TOP, and FOP plasmids, and Dr. L.K. Su for the APC expression plasmid. We would also like to thank Dr. A.G. de Herreros for the GST-β-catenin, Dr. P.J. Chiao for the pBS-p65 and pBS-p105 and MEF rela −/− cells, and Dr. J. Schmid for the GFP-p65 plasmid.",

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doi = "10.1016/S1535-6108(02)00154-X",

language = "English",

volume = "2",

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AU - Deng, Jiong

AU - Miller, Stephanie A.

AU - Wang, Hong Ying

AU - Xia, Weiya

AU - Wen, Yong

AU - Zhou, Binhua P.

AU - Li, Yan

AU - Lin, Shiaw Yih

AU - Hung, Mien Chie

N1 - Funding Information: This work was supported by NIH grants CA58880 and Ovarian SPORE grant P50 CA83639 (to M.-C. Hung), by a predoctoral fellowship from the US Army Breast Cancer Research Training Grant Program, Grant No. DAMD17-99-1-9264 (to Y. Wen), and Grant No. DAMD 17-02-1-0454 (to Y. Li). We would like to thank Dr. S.W. Byers for the wild type β-catenin and β-cateninS37A plasmids, Dr. H. Clevers for the dominant negative TCF4 (pcDNA3-ΔNTCF4), TOP, and FOP plasmids, and Dr. L.K. Su for the APC expression plasmid. We would also like to thank Dr. A.G. de Herreros for the GST-β-catenin, Dr. P.J. Chiao for the pBS-p65 and pBS-p105 and MEF rela −/− cells, and Dr. J. Schmid for the GFP-p65 plasmid.

PY - 2002/10

Y1 - 2002/10

N2 - β-catenin plays an important role in development and homeostasis. Deregulated β-catenin is involved in oncogenesis. In this study, we found that β-catenin can physically complex with NF-κB, resulting in a reduction of NF-κB DNA binding, transactivation activity, and target gene expression. Repressed NF-κB activity is found in human colon cancer cells in which β-catenin is activated. Importantly, activated β-catenin was found to inhibit the expression of NF-κB target genes, including Fas and TRAF1. Furthermore, a strong inverse correlation was identified between the expression levels of β-catenin and Fas in colon and breast tumor tissues, suggesting that β-catenin regulates NF-κB and its targets in vivo. Thus, β-catenin may play an important role in oncogenesis through the crossregulation of NF-κB.

AB - β-catenin plays an important role in development and homeostasis. Deregulated β-catenin is involved in oncogenesis. In this study, we found that β-catenin can physically complex with NF-κB, resulting in a reduction of NF-κB DNA binding, transactivation activity, and target gene expression. Repressed NF-κB activity is found in human colon cancer cells in which β-catenin is activated. Importantly, activated β-catenin was found to inhibit the expression of NF-κB target genes, including Fas and TRAF1. Furthermore, a strong inverse correlation was identified between the expression levels of β-catenin and Fas in colon and breast tumor tissues, suggesting that β-catenin regulates NF-κB and its targets in vivo. Thus, β-catenin may play an important role in oncogenesis through the crossregulation of NF-κB.

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β-catenin interacts with and inhibits NF-κB in human colon and breast cancer

Resumen

Nota bibliográfica

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

Acceder al documento

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