1,25(OH)2D3 regulates collagen quality in an osteoblastic cell culture system

Hideaki Nagaoka; Yoshiyuki Mochida; Phimon Atsawasuwan; Masaru Kaku; Toshirou Kondoh; Mitsuo Yamauchi

doi:10.1016/j.bbrc.2008.10.036

1,25(OH)₂D₃ regulates collagen quality in an osteoblastic cell culture system

Hideaki Nagaoka, Yoshiyuki Mochida, Phimon Atsawasuwan, Masaru Kaku, Toshirou Kondoh, Mitsuo Yamauchi

Oral Health Science

Producción científica: Article › revisión exhaustiva

36 Citas (Scopus)

Resumen

The active form of vitamin D, 1,25(OH)₂D₃, has a broad range of effects on bone, however, its role in the quality of bone matrix is not well understood. In this study, using an osteoblastic cell (MC3T3-E1) culture system, the effects of 1,25(OH)₂D₃ on collagen cross-linking and related enzymes, i.e., lysyl hydroxylases (LH1-3) and lysyl oxidases (LOX, LOXL1-4), were examined and compared to controls where cells were treated with cholecalciferol or ethanol. When compared to the controls, gene expressions of LH1, LH2b and LOXL2 were significantly upregulated by 1,25(OH)₂D₃ up to 72 h of culture. In addition, hydroxylysine (Hyl), Hyl aldehyde (Hyl^ald), Hyl^ald-derived cross-links and a total number of cross-links of collagen were significantly higher and the cross-link maturation was accelerated in the 1,25(OH)₂D₃ treated group. These results demonstrate that 1,25(OH)₂D₃ directly regulates collagen cross-linking in this culture system likely by upregulating gene expression of specific LH and LOX enzymes.

Idioma original	English
Páginas (desde-hasta)	674-678
Número de páginas	5
Publicación	Biochemical and Biophysical Research Communications
Volumen	377
N.º	2
DOI	https://doi.org/10.1016/j.bbrc.2008.10.036
Estado	Published - dic 12 2008

Nota bibliográfica

Funding Information:
This study was partially supported by NIH Grants NIH grant R01DE10489. The authors thank Drs. Ohta and Ishii at Teijin Pharma, Japan, for their valuable suggestions during the course of this study.

Financiación

This study was partially supported by NIH Grants NIH grant R01DE10489. The authors thank Drs. Ohta and Ishii at Teijin Pharma, Japan, for their valuable suggestions during the course of this study.

Financiadores	Número del financiador
National Institutes of Health (NIH)
National Institute of Dental and Craniofacial Research	R01DE010489

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Acceder al documento

10.1016/j.bbrc.2008.10.036

Otros archivos y enlaces

Citar esto

@article{a54864bfbd974aa9b41eb6ef5ea9e180,

title = "1,25(OH)2D3 regulates collagen quality in an osteoblastic cell culture system",

abstract = "The active form of vitamin D, 1,25(OH)2D3, has a broad range of effects on bone, however, its role in the quality of bone matrix is not well understood. In this study, using an osteoblastic cell (MC3T3-E1) culture system, the effects of 1,25(OH)2D3 on collagen cross-linking and related enzymes, i.e., lysyl hydroxylases (LH1-3) and lysyl oxidases (LOX, LOXL1-4), were examined and compared to controls where cells were treated with cholecalciferol or ethanol. When compared to the controls, gene expressions of LH1, LH2b and LOXL2 were significantly upregulated by 1,25(OH)2D3 up to 72 h of culture. In addition, hydroxylysine (Hyl), Hyl aldehyde (Hylald), Hylald-derived cross-links and a total number of cross-links of collagen were significantly higher and the cross-link maturation was accelerated in the 1,25(OH)2D3 treated group. These results demonstrate that 1,25(OH)2D3 directly regulates collagen cross-linking in this culture system likely by upregulating gene expression of specific LH and LOX enzymes.",

keywords = "Collagen cross-link, Collagen post-translational modification, MC3T3-E1, Vitamin D",

author = "Hideaki Nagaoka and Yoshiyuki Mochida and Phimon Atsawasuwan and Masaru Kaku and Toshirou Kondoh and Mitsuo Yamauchi",

note = "Funding Information: This study was partially supported by NIH Grants NIH grant R01DE10489. The authors thank Drs. Ohta and Ishii at Teijin Pharma, Japan, for their valuable suggestions during the course of this study.",

year = "2008",

month = dec,

day = "12",

doi = "10.1016/j.bbrc.2008.10.036",

language = "English",

volume = "377",

pages = "674--678",

number = "2",

}

TY - JOUR

T1 - 1,25(OH)2D3 regulates collagen quality in an osteoblastic cell culture system

AU - Nagaoka, Hideaki

AU - Mochida, Yoshiyuki

AU - Atsawasuwan, Phimon

AU - Kaku, Masaru

AU - Kondoh, Toshirou

AU - Yamauchi, Mitsuo

N1 - Funding Information: This study was partially supported by NIH Grants NIH grant R01DE10489. The authors thank Drs. Ohta and Ishii at Teijin Pharma, Japan, for their valuable suggestions during the course of this study.

PY - 2008/12/12

Y1 - 2008/12/12

N2 - The active form of vitamin D, 1,25(OH)2D3, has a broad range of effects on bone, however, its role in the quality of bone matrix is not well understood. In this study, using an osteoblastic cell (MC3T3-E1) culture system, the effects of 1,25(OH)2D3 on collagen cross-linking and related enzymes, i.e., lysyl hydroxylases (LH1-3) and lysyl oxidases (LOX, LOXL1-4), were examined and compared to controls where cells were treated with cholecalciferol or ethanol. When compared to the controls, gene expressions of LH1, LH2b and LOXL2 were significantly upregulated by 1,25(OH)2D3 up to 72 h of culture. In addition, hydroxylysine (Hyl), Hyl aldehyde (Hylald), Hylald-derived cross-links and a total number of cross-links of collagen were significantly higher and the cross-link maturation was accelerated in the 1,25(OH)2D3 treated group. These results demonstrate that 1,25(OH)2D3 directly regulates collagen cross-linking in this culture system likely by upregulating gene expression of specific LH and LOX enzymes.

AB - The active form of vitamin D, 1,25(OH)2D3, has a broad range of effects on bone, however, its role in the quality of bone matrix is not well understood. In this study, using an osteoblastic cell (MC3T3-E1) culture system, the effects of 1,25(OH)2D3 on collagen cross-linking and related enzymes, i.e., lysyl hydroxylases (LH1-3) and lysyl oxidases (LOX, LOXL1-4), were examined and compared to controls where cells were treated with cholecalciferol or ethanol. When compared to the controls, gene expressions of LH1, LH2b and LOXL2 were significantly upregulated by 1,25(OH)2D3 up to 72 h of culture. In addition, hydroxylysine (Hyl), Hyl aldehyde (Hylald), Hylald-derived cross-links and a total number of cross-links of collagen were significantly higher and the cross-link maturation was accelerated in the 1,25(OH)2D3 treated group. These results demonstrate that 1,25(OH)2D3 directly regulates collagen cross-linking in this culture system likely by upregulating gene expression of specific LH and LOX enzymes.

KW - Collagen cross-link

KW - Collagen post-translational modification

KW - MC3T3-E1

KW - Vitamin D

UR - http://www.scopus.com/inward/record.url?scp=55649115736&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=55649115736&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.10.036

DO - 10.1016/j.bbrc.2008.10.036

M3 - Article

C2 - 18930711

AN - SCOPUS:55649115736

SN - 0006-291X

VL - 377

SP - 674

EP - 678

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -