A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata

Xiaoling Ma; Ningxin Jiang; Jingxian Fu; Yuping Li; Lijun Zhou; Ling Yuan; Ying Wang; Yongqing Li

doi:10.1016/j.jplph.2023.154145

A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata

Xiaoling Ma
, Ningxin Jiang
, Jingxian Fu
, Yuping Li
, Lijun Zhou
, Ling Yuan
, Ying Wang
, Yongqing Li

Producción científica: Article › revisión exhaustiva

15 Citas (Scopus)

Resumen

Licochalcone A (LCA) is a characteristic compound of Glycyrrhiza inflata with anti-inflammatory, antioxidant and antitumor activities. However, G. inflata produces LCA in low quantities that does not meet the market demand. In this study, we found that DNA methylation inhibitor 5-azacitidine (5-azaC) successfully improved the LCA contents in G. inflata seedlings. Transcriptome analysis revealed a series of differentially expressed genes (DEGs), including transcription factors such as MYB, ERF, WRKY, and some structural genes related to flavonoid biosynthesis. However, whole genome bisulfite sequencing (BS-seq) results showed little effect of the 5-azaC treatment on the alteration of DNA methylation on these genes, indicating the possibility that 5-azaC acts as a stimulus, but not an epigenetic modulation factor to improve the LCA content in G. inflata. Additionally, we applied the 5-azaC treatment to field plants and hairy roots and successfully increased the LCA contents in both cases. This research demonstrates the feasibility of 5-azaC treatments in future applications to improve plant production of LCA.

Idioma original	English
Número de artículo	154145
Publicación	Journal of Plant Physiology
Volumen	292
DOI	https://doi.org/10.1016/j.jplph.2023.154145
Estado	Published - ene 2024

Nota bibliográfica

Publisher Copyright:
© 2023 Elsevier GmbH

Financiación

This work was supported by Biological Resources Program, Chinese Academy of Sciences (KFJ-BRP-007-017), Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (20230KJ148), the Foundation of Guangdong Provincial Key Laboratory of Applied Botany (2023B1212060046) & Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement (KF200203), South China Botanical Garden, Chinese Academy of Sciences (Granted No: QNXM-02), and China Postdoctoral Science Foundation (2020M682953 and 2021T140678).

Financiadores	Número del financiador
Biological Resources Program
Foundation of Guangdong Provincial Key Laboratory of Applied Botany	KF200203, 2023B1212060046
South China Botanical Garden	QNXM-02
Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams	20230KJ148
Chinese Academy of Sciences	KFJ-BRP-007-017
China Postdoctoral Science Foundation	2020M682953, 2021T140678

ASJC Scopus subject areas

Physiology
Agronomy and Crop Science
Plant Science

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@article{7a10dceec0264516a3e464949ad4c248,

title = "A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata",

abstract = "Licochalcone A (LCA) is a characteristic compound of Glycyrrhiza inflata with anti-inflammatory, antioxidant and antitumor activities. However, G. inflata produces LCA in low quantities that does not meet the market demand. In this study, we found that DNA methylation inhibitor 5-azacitidine (5-azaC) successfully improved the LCA contents in G. inflata seedlings. Transcriptome analysis revealed a series of differentially expressed genes (DEGs), including transcription factors such as MYB, ERF, WRKY, and some structural genes related to flavonoid biosynthesis. However, whole genome bisulfite sequencing (BS-seq) results showed little effect of the 5-azaC treatment on the alteration of DNA methylation on these genes, indicating the possibility that 5-azaC acts as a stimulus, but not an epigenetic modulation factor to improve the LCA content in G. inflata. Additionally, we applied the 5-azaC treatment to field plants and hairy roots and successfully increased the LCA contents in both cases. This research demonstrates the feasibility of 5-azaC treatments in future applications to improve plant production of LCA.",

keywords = "5-Azacitidine, DNA methylation inhibitor, Flavonoid, Glycyrrhiza inflata, Licochalcone A",

author = "Xiaoling Ma and Ningxin Jiang and Jingxian Fu and Yuping Li and Lijun Zhou and Ling Yuan and Ying Wang and Yongqing Li",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier GmbH",

year = "2024",

month = jan,

doi = "10.1016/j.jplph.2023.154145",

language = "English",

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TY - JOUR

T1 - A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata

AU - Ma, Xiaoling

AU - Jiang, Ningxin

AU - Fu, Jingxian

AU - Li, Yuping

AU - Zhou, Lijun

AU - Yuan, Ling

AU - Wang, Ying

AU - Li, Yongqing

PY - 2024/1

Y1 - 2024/1

N2 - Licochalcone A (LCA) is a characteristic compound of Glycyrrhiza inflata with anti-inflammatory, antioxidant and antitumor activities. However, G. inflata produces LCA in low quantities that does not meet the market demand. In this study, we found that DNA methylation inhibitor 5-azacitidine (5-azaC) successfully improved the LCA contents in G. inflata seedlings. Transcriptome analysis revealed a series of differentially expressed genes (DEGs), including transcription factors such as MYB, ERF, WRKY, and some structural genes related to flavonoid biosynthesis. However, whole genome bisulfite sequencing (BS-seq) results showed little effect of the 5-azaC treatment on the alteration of DNA methylation on these genes, indicating the possibility that 5-azaC acts as a stimulus, but not an epigenetic modulation factor to improve the LCA content in G. inflata. Additionally, we applied the 5-azaC treatment to field plants and hairy roots and successfully increased the LCA contents in both cases. This research demonstrates the feasibility of 5-azaC treatments in future applications to improve plant production of LCA.

AB - Licochalcone A (LCA) is a characteristic compound of Glycyrrhiza inflata with anti-inflammatory, antioxidant and antitumor activities. However, G. inflata produces LCA in low quantities that does not meet the market demand. In this study, we found that DNA methylation inhibitor 5-azacitidine (5-azaC) successfully improved the LCA contents in G. inflata seedlings. Transcriptome analysis revealed a series of differentially expressed genes (DEGs), including transcription factors such as MYB, ERF, WRKY, and some structural genes related to flavonoid biosynthesis. However, whole genome bisulfite sequencing (BS-seq) results showed little effect of the 5-azaC treatment on the alteration of DNA methylation on these genes, indicating the possibility that 5-azaC acts as a stimulus, but not an epigenetic modulation factor to improve the LCA content in G. inflata. Additionally, we applied the 5-azaC treatment to field plants and hairy roots and successfully increased the LCA contents in both cases. This research demonstrates the feasibility of 5-azaC treatments in future applications to improve plant production of LCA.

KW - 5-Azacitidine

KW - DNA methylation inhibitor

KW - Flavonoid

KW - Glycyrrhiza inflata

KW - Licochalcone A

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DO - 10.1016/j.jplph.2023.154145

M3 - Article

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SN - 0176-1617

VL - 292

JO - Journal of Plant Physiology

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A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata

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