A FLUOROMETRIC ASSAY FOR CHOLINE ACETYLTRANSFERASE and ITS USE IN THE PURIFICATION OF THE ENZYME FROM HUMAN PLACENTA

Louis B. Hersh; Barbara Coe; Lynette Casey

doi:10.1111/j.1471-4159.1978.tb12401.x

A FLUOROMETRIC ASSAY FOR CHOLINE ACETYLTRANSFERASE and ITS USE IN THE PURIFICATION OF THE ENZYME FROM HUMAN PLACENTA

Louis B. Hersh
, Barbara Coe
, Lynette Casey

Producción científica: Article › revisión exhaustiva

41 Citas (Scopus)

Resumen

Abstract— A fluorometric assay for choline acetyltransferase has been developed. This assay is based on coupling the choline acetyltransferase dependent formation of acetyl‐CoA from acetylcholine and coenzyme A, to the reactions catalyzed by the enzymes citrate synthase and malic dehydrogenase. Although this assay is not as sensitive as previously described radiometric assays, it can be conveniently used during enzyme purification. Employing this assay method, choline acetyltransferase has been purified from human placenta to a specific activity of 92.7 μmol acetylcholine formed/min/mg protein.

Idioma original	English
Páginas (desde-hasta)	1077-1085
Número de páginas	9
Publicación	Journal of Neurochemistry
Volumen	30
N.º	5
DOI	https://doi.org/10.1111/j.1471-4159.1978.tb12401.x
Estado	Published - may 1978

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1978.tb12401.x

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abstract = "Abstract— A fluorometric assay for choline acetyltransferase has been developed. This assay is based on coupling the choline acetyltransferase dependent formation of acetyl‐CoA from acetylcholine and coenzyme A, to the reactions catalyzed by the enzymes citrate synthase and malic dehydrogenase. Although this assay is not as sensitive as previously described radiometric assays, it can be conveniently used during enzyme purification. Employing this assay method, choline acetyltransferase has been purified from human placenta to a specific activity of 92.7 μmol acetylcholine formed/min/mg protein.",

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AU - Casey, Lynette

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N2 - Abstract— A fluorometric assay for choline acetyltransferase has been developed. This assay is based on coupling the choline acetyltransferase dependent formation of acetyl‐CoA from acetylcholine and coenzyme A, to the reactions catalyzed by the enzymes citrate synthase and malic dehydrogenase. Although this assay is not as sensitive as previously described radiometric assays, it can be conveniently used during enzyme purification. Employing this assay method, choline acetyltransferase has been purified from human placenta to a specific activity of 92.7 μmol acetylcholine formed/min/mg protein.

AB - Abstract— A fluorometric assay for choline acetyltransferase has been developed. This assay is based on coupling the choline acetyltransferase dependent formation of acetyl‐CoA from acetylcholine and coenzyme A, to the reactions catalyzed by the enzymes citrate synthase and malic dehydrogenase. Although this assay is not as sensitive as previously described radiometric assays, it can be conveniently used during enzyme purification. Employing this assay method, choline acetyltransferase has been purified from human placenta to a specific activity of 92.7 μmol acetylcholine formed/min/mg protein.

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A FLUOROMETRIC ASSAY FOR CHOLINE ACETYLTRANSFERASE and ITS USE IN THE PURIFICATION OF THE ENZYME FROM HUMAN PLACENTA

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