A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

Shanteri Singh; Pauline Peltier-Pain; Marco Tonelli; Jon S. Thorson

doi:10.1021/ol501241a

A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

Shanteri Singh
, Pauline Peltier-Pain
, Marco Tonelli
, Jon S. Thorson

Producción científica: Article › revisión exhaustiva

12 Citas (Scopus)

Resumen

A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively ¹³C-labeled sugar nucleotides and inverse ¹³C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.

Idioma original	English
Páginas (desde-hasta)	3220-3223
Número de páginas	4
Publicación	Organic Letters
Volumen	16
N.º	12
DOI	https://doi.org/10.1021/ol501241a
Estado	Published - jun 20 2014

Financiación

Financiadores	Número del financiador
National Center for Advancing Translational Sciences (NCATS)	UL1TR000117
National Institutes of Health (NIH)	RR02781, RR08438, P41GM10399, P41RR02301, RO1 CA84374, R37 AI52218
National Stroke Foundation	DMB-8415048, BIR-9214394, OIA-9977486
U.S. Department of Agriculture
Michigan State University-U.S. Department of Energy (MSU-DOE) Plant Research Laboratory
National Institute of General Medical Sciences	F32GM010399

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol501241a

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abstract = "A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively 13C-labeled sugar nucleotides and inverse 13C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.",

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AU - Tonelli, Marco

AU - Thorson, Jon S.

PY - 2014/6/20

Y1 - 2014/6/20

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AB - A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively 13C-labeled sugar nucleotides and inverse 13C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.

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A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

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ASJC Scopus subject areas

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