A new family of cytokinin receptors from cereales

O. N. Kulaeva; T. K. Zagranichnaya; F. A. Brovko; N. N. Karavaiko; S. Yu Selivankina; Ya V. Zemlyachenko; M. Hall; V. M. Lipkin; Kh M. Boziev

doi:10.1016/S0014-5793(98)00101-X

A new family of cytokinin receptors from cereales

O. N. Kulaeva
, T. K. Zagranichnaya
, F. A. Brovko
, N. N. Karavaiko
, S. Yu Selivankina
, Ya V. Zemlyachenko
, M. Hall
, V. M. Lipkin
, Kh M. Boziev

Internal Medicine

Producción científica: Article › revisión exhaustiva

25 Citas (Scopus)

Resumen

The highly specific recognition of a natural cytokinin, trans-zeatin, by cytokinin-binding protein (CBP) of 67 kDa from barley leaves was detected with an assay developed on the basis of cytokinin competition in ELISA with anti-idiotype antibodies (raised against antibodies to zeatin) for complex formation with CBP. Monoclonal antibodies (mAbs) raised against 70 kDa CBP from etiolated maize seedlings cross-reacted with barley 67 kDa CBP and prevented barley CBP and trans-zeatin induced activation of transcription elongation directed by RNA polymerase I associated with barley chromatin. One mAb (Z-6) had an agonistic effect. Maize CBP replaced barley CBP in activation of RNA synthesis with cytokinin in the barley transcription system. Hence, a new family of cytokinin receptors with common functions and immunodeterminants including maize and barley CBPs was found.

Idioma original	English
Páginas (desde-hasta)	239-242
Número de páginas	4
Publicación	FEBS Letters
Volumen	423
N.º	2
DOI	https://doi.org/10.1016/S0014-5793(98)00101-X
Estado	Published - feb 20 1998

Nota bibliográfica

Funding Information:
We are very grateful to Dr. J. Corse for the gift of zeatin- O -glucoside. This work was partially supported by the Russian Foundation for Fundamental Research (Grants 96-04-50705, 96-04-00128 G and 96-15-98030) and by INTAS Grant 93-678 EXT.

Financiación

We are very grateful to Dr. J. Corse for the gift of zeatin- O -glucoside. This work was partially supported by the Russian Foundation for Fundamental Research (Grants 96-04-50705, 96-04-00128 G and 96-15-98030) and by INTAS Grant 93-678 EXT.

Financiadores	Número del financiador
INTAS, Belgium	93-678 EXT
Russian Foundation for Fundamental Investigations	96-04-50705, 96-04-00128 G, 96-15-98030

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(98)00101-X

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title = "A new family of cytokinin receptors from cereales",

abstract = "The highly specific recognition of a natural cytokinin, trans-zeatin, by cytokinin-binding protein (CBP) of 67 kDa from barley leaves was detected with an assay developed on the basis of cytokinin competition in ELISA with anti-idiotype antibodies (raised against antibodies to zeatin) for complex formation with CBP. Monoclonal antibodies (mAbs) raised against 70 kDa CBP from etiolated maize seedlings cross-reacted with barley 67 kDa CBP and prevented barley CBP and trans-zeatin induced activation of transcription elongation directed by RNA polymerase I associated with barley chromatin. One mAb (Z-6) had an agonistic effect. Maize CBP replaced barley CBP in activation of RNA synthesis with cytokinin in the barley transcription system. Hence, a new family of cytokinin receptors with common functions and immunodeterminants including maize and barley CBPs was found.",

keywords = "Anti-idiotype antibody, Chromatin, Cytokinin-binding protein, Monoclonal antibody, RNA polymerase I",

author = "Kulaeva, \{O. N.\} and Zagranichnaya, \{T. K.\} and Brovko, \{F. A.\} and Karavaiko, \{N. N.\} and Selivankina, \{S. Yu\} and Zemlyachenko, \{Ya V.\} and M. Hall and Lipkin, \{V. M.\} and Boziev, \{Kh M.\}",

note = "Funding Information: We are very grateful to Dr. J. Corse for the gift of zeatin- O -glucoside. This work was partially supported by the Russian Foundation for Fundamental Research (Grants 96-04-50705, 96-04-00128 G and 96-15-98030) and by INTAS Grant 93-678 EXT. ",

year = "1998",

month = feb,

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doi = "10.1016/S0014-5793(98)00101-X",

language = "English",

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AU - Kulaeva, O. N.

AU - Zagranichnaya, T. K.

AU - Brovko, F. A.

AU - Karavaiko, N. N.

AU - Selivankina, S. Yu

AU - Zemlyachenko, Ya V.

AU - Hall, M.

AU - Lipkin, V. M.

AU - Boziev, Kh M.

N1 - Funding Information: We are very grateful to Dr. J. Corse for the gift of zeatin- O -glucoside. This work was partially supported by the Russian Foundation for Fundamental Research (Grants 96-04-50705, 96-04-00128 G and 96-15-98030) and by INTAS Grant 93-678 EXT.

PY - 1998/2/20

Y1 - 1998/2/20

N2 - The highly specific recognition of a natural cytokinin, trans-zeatin, by cytokinin-binding protein (CBP) of 67 kDa from barley leaves was detected with an assay developed on the basis of cytokinin competition in ELISA with anti-idiotype antibodies (raised against antibodies to zeatin) for complex formation with CBP. Monoclonal antibodies (mAbs) raised against 70 kDa CBP from etiolated maize seedlings cross-reacted with barley 67 kDa CBP and prevented barley CBP and trans-zeatin induced activation of transcription elongation directed by RNA polymerase I associated with barley chromatin. One mAb (Z-6) had an agonistic effect. Maize CBP replaced barley CBP in activation of RNA synthesis with cytokinin in the barley transcription system. Hence, a new family of cytokinin receptors with common functions and immunodeterminants including maize and barley CBPs was found.

AB - The highly specific recognition of a natural cytokinin, trans-zeatin, by cytokinin-binding protein (CBP) of 67 kDa from barley leaves was detected with an assay developed on the basis of cytokinin competition in ELISA with anti-idiotype antibodies (raised against antibodies to zeatin) for complex formation with CBP. Monoclonal antibodies (mAbs) raised against 70 kDa CBP from etiolated maize seedlings cross-reacted with barley 67 kDa CBP and prevented barley CBP and trans-zeatin induced activation of transcription elongation directed by RNA polymerase I associated with barley chromatin. One mAb (Z-6) had an agonistic effect. Maize CBP replaced barley CBP in activation of RNA synthesis with cytokinin in the barley transcription system. Hence, a new family of cytokinin receptors with common functions and immunodeterminants including maize and barley CBPs was found.

KW - Anti-idiotype antibody

KW - Chromatin

KW - Cytokinin-binding protein

KW - Monoclonal antibody

KW - RNA polymerase I

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A new family of cytokinin receptors from cereales

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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Huella

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