A New Method for Quantitative Determination of Poly-3-hydroxybutyrate and 3-Hydroxybutyrate-3-hydroxyvalerate Copolymer in Microbial Biomass by Reversed-Phase High-Performance Liquid Chromatography

N. A. Korotkova; V. V. Ashin; N. V. Doronina; Yu A. Trotsenko

A New Method for Quantitative Determination of Poly-3-hydroxybutyrate and 3-Hydroxybutyrate-3-hydroxyvalerate Copolymer in Microbial Biomass by Reversed-Phase High-Performance Liquid Chromatography

N. A. Korotkova
, V. V. Ashin
, N. V. Doronina
, Yu A. Trotsenko

Producción científica: Article › revisión exhaustiva

4 Citas (Scopus)

Resumen

A new sensitive method for quantitative determination of poly-3-hydroxybutyrate (PHB) and 3-hydroxybutyrate-3-hydroxyvalerate copolymer (PHBV) in microbial biomass by isocratic reversed-phase high-performance liquid chromatography was developed. The method employs chromatographic analysis of crotonic acid formed by PHB hydrolysis with concentrated sulfuric acid or crotonic and methylcrotonic acids formed by PHBV hydrolysis with 1 N NaOH.

Idioma original	English
Páginas (desde-hasta)	342-343
Número de páginas	2
Publicación	Prikladnaya Biokhimiya i Mikrobiologiya
Volumen	33
N.º	3
Estado	Published - mar 1997

ASJC Scopus subject areas

General Medicine

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title = "A New Method for Quantitative Determination of Poly-3-hydroxybutyrate and 3-Hydroxybutyrate-3-hydroxyvalerate Copolymer in Microbial Biomass by Reversed-Phase High-Performance Liquid Chromatography",

abstract = "A new sensitive method for quantitative determination of poly-3-hydroxybutyrate (PHB) and 3-hydroxybutyrate-3-hydroxyvalerate copolymer (PHBV) in microbial biomass by isocratic reversed-phase high-performance liquid chromatography was developed. The method employs chromatographic analysis of crotonic acid formed by PHB hydrolysis with concentrated sulfuric acid or crotonic and methylcrotonic acids formed by PHBV hydrolysis with 1 N NaOH.",

author = "Korotkova, \{N. A.\} and Ashin, \{V. V.\} and Doronina, \{N. V.\} and Trotsenko, \{Yu A.\}",

year = "1997",

month = mar,

language = "English",

volume = "33",

pages = "342--343",

number = "3",

}

A New Method for Quantitative Determination of Poly-3-hydroxybutyrate and 3-Hydroxybutyrate-3-hydroxyvalerate Copolymer in Microbial Biomass by Reversed-Phase High-Performance Liquid Chromatography. / Korotkova, N. A.; Ashin, V. V.; Doronina, N. V. et al.
En: Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 33, N.º 3, 03.1997, p. 342-343.

Producción científica: Article › revisión exhaustiva

TY - JOUR

T1 - A New Method for Quantitative Determination of Poly-3-hydroxybutyrate and 3-Hydroxybutyrate-3-hydroxyvalerate Copolymer in Microbial Biomass by Reversed-Phase High-Performance Liquid Chromatography

AU - Korotkova, N. A.

AU - Ashin, V. V.

AU - Doronina, N. V.

AU - Trotsenko, Yu A.

PY - 1997/3

Y1 - 1997/3

N2 - A new sensitive method for quantitative determination of poly-3-hydroxybutyrate (PHB) and 3-hydroxybutyrate-3-hydroxyvalerate copolymer (PHBV) in microbial biomass by isocratic reversed-phase high-performance liquid chromatography was developed. The method employs chromatographic analysis of crotonic acid formed by PHB hydrolysis with concentrated sulfuric acid or crotonic and methylcrotonic acids formed by PHBV hydrolysis with 1 N NaOH.

AB - A new sensitive method for quantitative determination of poly-3-hydroxybutyrate (PHB) and 3-hydroxybutyrate-3-hydroxyvalerate copolymer (PHBV) in microbial biomass by isocratic reversed-phase high-performance liquid chromatography was developed. The method employs chromatographic analysis of crotonic acid formed by PHB hydrolysis with concentrated sulfuric acid or crotonic and methylcrotonic acids formed by PHBV hydrolysis with 1 N NaOH.

UR - https://www.scopus.com/pages/publications/0347173471

UR - https://www.scopus.com/pages/publications/0347173471#tab=citedBy

M3 - Article

AN - SCOPUS:0347173471

SN - 0555-1099

VL - 33

SP - 342

EP - 343

JO - Prikladnaya Biokhimiya i Mikrobiologiya

JF - Prikladnaya Biokhimiya i Mikrobiologiya

IS - 3

ER -

A New Method for Quantitative Determination of Poly-3-hydroxybutyrate and 3-Hydroxybutyrate-3-hydroxyvalerate Copolymer in Microbial Biomass by Reversed-Phase High-Performance Liquid Chromatography

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ASJC Scopus subject areas

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