TY - JOUR
T1 - Amyloid β-peptide spin trapping II
T2 - Evidence for decomposition of the pbn spin adduct
AU - Hensley, Kenneth
AU - Aksenova, Marina
AU - Carney, John M.
AU - Harris, Marni
AU - Butterfield, D. Allan
PY - 1995/2
Y1 - 1995/2
N2 - SYNTHETIC β-amyloid peptides (Aβs) react with the spin trap phenyl-terf-hutyl nitrone (PBN) to form products detectable by electron paramagnetic resonance (EPR) spectroscopy. At least two EPR-detectable products can be distinguished from the Aβ/PBN reaction, and peptide toxicity towards glutamine synthetase enzyme correlates with the type of PBN reaction product observed. We have reacted synthetic Aβ(25-35) peptide with [12C]- or [13C]PBN to demonstrate that the two products represent alternate pathways of spin adduct decomposition. Results indicate that the C=N bond of PBN is cleaved by Aβ in what we hypothesize is a radical addition-fragmentation reaction.
AB - SYNTHETIC β-amyloid peptides (Aβs) react with the spin trap phenyl-terf-hutyl nitrone (PBN) to form products detectable by electron paramagnetic resonance (EPR) spectroscopy. At least two EPR-detectable products can be distinguished from the Aβ/PBN reaction, and peptide toxicity towards glutamine synthetase enzyme correlates with the type of PBN reaction product observed. We have reacted synthetic Aβ(25-35) peptide with [12C]- or [13C]PBN to demonstrate that the two products represent alternate pathways of spin adduct decomposition. Results indicate that the C=N bond of PBN is cleaved by Aβ in what we hypothesize is a radical addition-fragmentation reaction.
KW - Amyloid
KW - Oxidation
KW - Phenyl-tert-butyl nitrone
KW - Spin trapping
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U2 - 10.1097/00001756-199502000-00022
DO - 10.1097/00001756-199502000-00022
M3 - Article
C2 - 7766851
AN - SCOPUS:0028985209
SN - 0959-4965
VL - 6
SP - 493
EP - 496
JO - NeuroReport
JF - NeuroReport
IS - 3
ER -