An Improved Filtration Procedure for Measuring Opiate Receptors in Small Regions of Rat Brain

M. T. Bardo; R. K. Bhatnagar; G. F. Gebhart

doi:10.1111/j.1471-4159.1982.tb08015.x

An Improved Filtration Procedure for Measuring Opiate Receptors in Small Regions of Rat Brain

M. T. Bardo
, R. K. Bhatnagar
, G. F. Gebhart

Producción científica: Article › revisión exhaustiva

13 Citas (Scopus)

Resumen

Abstract: A modified filtration method for in vitro receptor binding was used to determine specific binding of [³H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 μg of protein per assay tube. Large differences in [³H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.

Idioma original	English
Páginas (desde-hasta)	1751-1754
Número de páginas	4
Publicación	Journal of Neurochemistry
Volumen	39
N.º	6
DOI	https://doi.org/10.1111/j.1471-4159.1982.tb08015.x
Estado	Published - dic 1982

Financiación

Financiadores	Número del financiador
National Institute on Drug Abuse	R37DA002879

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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title = "An Improved Filtration Procedure for Measuring Opiate Receptors in Small Regions of Rat Brain",

abstract = "Abstract: A modified filtration method for in vitro receptor binding was used to determine specific binding of [3H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 μg of protein per assay tube. Large differences in [3H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.",

keywords = "Brain nuclei, Naloxone, Opiate receptor",

author = "Bardo, \{M. T.\} and Bhatnagar, \{R. K.\} and Gebhart, \{G. F.\}",

year = "1982",

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AU - Bardo, M. T.

AU - Bhatnagar, R. K.

AU - Gebhart, G. F.

PY - 1982/12

Y1 - 1982/12

N2 - Abstract: A modified filtration method for in vitro receptor binding was used to determine specific binding of [3H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 μg of protein per assay tube. Large differences in [3H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.

AB - Abstract: A modified filtration method for in vitro receptor binding was used to determine specific binding of [3H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 μg of protein per assay tube. Large differences in [3H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.

KW - Brain nuclei

KW - Naloxone

KW - Opiate receptor

UR - https://www.scopus.com/pages/publications/0020365320

UR - https://www.scopus.com/pages/publications/0020365320#tab=citedBy

U2 - 10.1111/j.1471-4159.1982.tb08015.x

DO - 10.1111/j.1471-4159.1982.tb08015.x

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AN - SCOPUS:0020365320

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SP - 1751

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JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

IS - 6

ER -

An Improved Filtration Procedure for Measuring Opiate Receptors in Small Regions of Rat Brain

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