An Intact Cuticle in Distal Tissues Is Essential for the Induction of Systemic Acquired Resistance in Plants

Ye Xia; Qing Ming Gao; Keshun Yu; Ludmila Lapchyk; Du Roy Navarre; David Hildebrand; Aardra Kachroo; Pradeep Kachroo

doi:10.1016/j.chom.2009.01.001

An Intact Cuticle in Distal Tissues Is Essential for the Induction of Systemic Acquired Resistance in Plants

Ye Xia
, Qing Ming Gao
, Keshun Yu
, Ludmila Lapchyk
, Du Roy Navarre
, David Hildebrand
, Aardra Kachroo
, Pradeep Kachroo

Producción científica: Article › revisión exhaustiva

121 Citas (Scopus)

Resumen

Systemic acquired resistance (SAR), initiated by a plant upon recognition of microbial effectors, involves generation of a mobile signal at the primary infection site, which translocates to and activates defense responses in distal tissues via unknown mechanism(s). We find that an acyl carrier protein, ACP4, is required to perceive the mobile SAR signal in distal tissues of Arabidopsis. Although acp4 plants generated the mobile signal, they failed to induce the systemic immunity response. Defective SAR in acp4 plants was not due to impairment in salicylic acid (SA)-, methyl SA-, or jasmonic acid-mediated plant hormone signaling pathways but was associated with the impaired cuticle of acp4 leaves. Other cuticle-impairing genetic mutations or physical removal of the cuticle also compromised SAR. This cuticular requirement was relevant only during mobile signal generation and its translocation to distal tissues. Collectively, these data suggest an active role for the plant cuticle in SAR-related molecular signaling.

Idioma original	English
Páginas (desde-hasta)	151-165
Número de páginas	15
Publicación	Cell Host and Microbe
Volumen	5
N.º	2
DOI	https://doi.org/10.1016/j.chom.2009.01.001
Estado	Published - feb 19 2009

Nota bibliográfica

Funding Information:
We thank John Johnson for help with gas chromatography, Larry Rice for help with SEM, Mary Gail Engle for help with TEM, Claire Venard for help with thick sectioning, Rae-Dong Jeong for quantification of R gene levels, and Thomas Muse and Lev Orlov for help with fatty acid extractions and genotyping. We also thank Mike Pollard for cutin monomer spectra and useful advice, John Browse for lacs2 and lacs9 seeds, and Walter Grassmann for Pseudomonas syringae strain containing AvrRPS4. We thank Kansas Lipidomics Research Center Analytical Laboratory and its support from National Science Foundation's EPSCoR program, under grant number EPS-0236913 with matching support from the State of Kansas through Kansas Technology Enterprise Corporation and Kansas State University. This work was supported by grants from NSF (MCB#0421914), USDA-NRI (2004-03287), and KSEF (419-RDE-004, 04RDE-006, 820-RDE-007).

Financiación

We thank John Johnson for help with gas chromatography, Larry Rice for help with SEM, Mary Gail Engle for help with TEM, Claire Venard for help with thick sectioning, Rae-Dong Jeong for quantification of R gene levels, and Thomas Muse and Lev Orlov for help with fatty acid extractions and genotyping. We also thank Mike Pollard for cutin monomer spectra and useful advice, John Browse for lacs2 and lacs9 seeds, and Walter Grassmann for Pseudomonas syringae strain containing AvrRPS4. We thank Kansas Lipidomics Research Center Analytical Laboratory and its support from National Science Foundation's EPSCoR program, under grant number EPS-0236913 with matching support from the State of Kansas through Kansas Technology Enterprise Corporation and Kansas State University. This work was supported by grants from NSF (MCB#0421914), USDA-NRI (2004-03287), and KSEF (419-RDE-004, 04RDE-006, 820-RDE-007).

Financiadores	Número del financiador
Kansas Lipidomics Research Center Analytical Laboratory
Kansas Technology Enterprise Corporation
National Science Foundation's EPSCoR program	EPS-0236913
Kansas State University
USDA-CSREES-NRI-funded	2004-03287
National Science Foundation Arctic Social Science Program	0421914
Kentucky Science and Energy Foundation	04RDE-006, 820-RDE-007, 419-RDE-004

ASJC Scopus subject areas

Parasitology
Microbiology
Virology

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title = "An Intact Cuticle in Distal Tissues Is Essential for the Induction of Systemic Acquired Resistance in Plants",

abstract = "Systemic acquired resistance (SAR), initiated by a plant upon recognition of microbial effectors, involves generation of a mobile signal at the primary infection site, which translocates to and activates defense responses in distal tissues via unknown mechanism(s). We find that an acyl carrier protein, ACP4, is required to perceive the mobile SAR signal in distal tissues of Arabidopsis. Although acp4 plants generated the mobile signal, they failed to induce the systemic immunity response. Defective SAR in acp4 plants was not due to impairment in salicylic acid (SA)-, methyl SA-, or jasmonic acid-mediated plant hormone signaling pathways but was associated with the impaired cuticle of acp4 leaves. Other cuticle-impairing genetic mutations or physical removal of the cuticle also compromised SAR. This cuticular requirement was relevant only during mobile signal generation and its translocation to distal tissues. Collectively, these data suggest an active role for the plant cuticle in SAR-related molecular signaling.",

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author = "Ye Xia and Gao, \{Qing Ming\} and Keshun Yu and Ludmila Lapchyk and Navarre, \{Du Roy\} and David Hildebrand and Aardra Kachroo and Pradeep Kachroo",

note = "Funding Information: We thank John Johnson for help with gas chromatography, Larry Rice for help with SEM, Mary Gail Engle for help with TEM, Claire Venard for help with thick sectioning, Rae-Dong Jeong for quantification of R gene levels, and Thomas Muse and Lev Orlov for help with fatty acid extractions and genotyping. We also thank Mike Pollard for cutin monomer spectra and useful advice, John Browse for lacs2 and lacs9 seeds, and Walter Grassmann for Pseudomonas syringae strain containing AvrRPS4. We thank Kansas Lipidomics Research Center Analytical Laboratory and its support from National Science Foundation's EPSCoR program, under grant number EPS-0236913 with matching support from the State of Kansas through Kansas Technology Enterprise Corporation and Kansas State University. This work was supported by grants from NSF (MCB\#0421914), USDA-NRI (2004-03287), and KSEF (419-RDE-004, 04RDE-006, 820-RDE-007). ",

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AU - Xia, Ye

AU - Gao, Qing Ming

AU - Yu, Keshun

AU - Lapchyk, Ludmila

AU - Navarre, Du Roy

AU - Hildebrand, David

AU - Kachroo, Aardra

AU - Kachroo, Pradeep

N1 - Funding Information: We thank John Johnson for help with gas chromatography, Larry Rice for help with SEM, Mary Gail Engle for help with TEM, Claire Venard for help with thick sectioning, Rae-Dong Jeong for quantification of R gene levels, and Thomas Muse and Lev Orlov for help with fatty acid extractions and genotyping. We also thank Mike Pollard for cutin monomer spectra and useful advice, John Browse for lacs2 and lacs9 seeds, and Walter Grassmann for Pseudomonas syringae strain containing AvrRPS4. We thank Kansas Lipidomics Research Center Analytical Laboratory and its support from National Science Foundation's EPSCoR program, under grant number EPS-0236913 with matching support from the State of Kansas through Kansas Technology Enterprise Corporation and Kansas State University. This work was supported by grants from NSF (MCB#0421914), USDA-NRI (2004-03287), and KSEF (419-RDE-004, 04RDE-006, 820-RDE-007).

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Y1 - 2009/2/19

N2 - Systemic acquired resistance (SAR), initiated by a plant upon recognition of microbial effectors, involves generation of a mobile signal at the primary infection site, which translocates to and activates defense responses in distal tissues via unknown mechanism(s). We find that an acyl carrier protein, ACP4, is required to perceive the mobile SAR signal in distal tissues of Arabidopsis. Although acp4 plants generated the mobile signal, they failed to induce the systemic immunity response. Defective SAR in acp4 plants was not due to impairment in salicylic acid (SA)-, methyl SA-, or jasmonic acid-mediated plant hormone signaling pathways but was associated with the impaired cuticle of acp4 leaves. Other cuticle-impairing genetic mutations or physical removal of the cuticle also compromised SAR. This cuticular requirement was relevant only during mobile signal generation and its translocation to distal tissues. Collectively, these data suggest an active role for the plant cuticle in SAR-related molecular signaling.

AB - Systemic acquired resistance (SAR), initiated by a plant upon recognition of microbial effectors, involves generation of a mobile signal at the primary infection site, which translocates to and activates defense responses in distal tissues via unknown mechanism(s). We find that an acyl carrier protein, ACP4, is required to perceive the mobile SAR signal in distal tissues of Arabidopsis. Although acp4 plants generated the mobile signal, they failed to induce the systemic immunity response. Defective SAR in acp4 plants was not due to impairment in salicylic acid (SA)-, methyl SA-, or jasmonic acid-mediated plant hormone signaling pathways but was associated with the impaired cuticle of acp4 leaves. Other cuticle-impairing genetic mutations or physical removal of the cuticle also compromised SAR. This cuticular requirement was relevant only during mobile signal generation and its translocation to distal tissues. Collectively, these data suggest an active role for the plant cuticle in SAR-related molecular signaling.

KW - MICROBIO

KW - MOLIMMUNO

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JO - Cell Host and Microbe

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ER -

An Intact Cuticle in Distal Tissues Is Essential for the Induction of Systemic Acquired Resistance in Plants

Resumen

Nota bibliográfica

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ASJC Scopus subject areas

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