TY - JOUR
T1 - Anaplastic large cell lymphoma
T2 - A flow cytometric analysis of 29 cases
AU - Kesler, Melissa V.
AU - Paranjape, Geeta S.
AU - Asplund, Sheryl L.
AU - McKenna, Robert W.
AU - Jamal, Saba
AU - Kroft, Steven H.
PY - 2007/8
Y1 - 2007/8
N2 - We report our experience with flow cytometric (FC) analysis of 29 cases of anaplastic large cell lymphoma (ALCL). Morphologic analysis of processed cytocentrifuged preparations demonstrated neoplastic cells in 28 cases. In 25 of these, an aberrant lymphoid population was detected by FC analysis. The majority showed high orthogonal light scatter, similar to monocytes or granulocytes. Of the antigens CD2, CD3, CD4, CD5, and CD7, 5 cases expressed 1, 8 expressed 2, 6 expressed 3, 3 expressed 4, and 3 expressed all 5. CD4 was expressed most commonly (20/25 [80%]), followed by CD2 (18/25 [72%]), CD3 (10/25 [40%]), and CD5 and CD7 (8/25 [32%] each). CD45 was expressed in 23 of 25 cases and CD13 in 7 of 9. Of 21 cases, 13 were anaplastic lymphoma kinase (ALK)+, all of which were CD4+, vs 5 of 8 ALK- cases (P = .042). Most ALCLs can be detected and characterized by multiparameter FC analysis. However, light scatter gating on typical lymphoid regions may yield false-negative results in a substantial number of cases.
AB - We report our experience with flow cytometric (FC) analysis of 29 cases of anaplastic large cell lymphoma (ALCL). Morphologic analysis of processed cytocentrifuged preparations demonstrated neoplastic cells in 28 cases. In 25 of these, an aberrant lymphoid population was detected by FC analysis. The majority showed high orthogonal light scatter, similar to monocytes or granulocytes. Of the antigens CD2, CD3, CD4, CD5, and CD7, 5 cases expressed 1, 8 expressed 2, 6 expressed 3, 3 expressed 4, and 3 expressed all 5. CD4 was expressed most commonly (20/25 [80%]), followed by CD2 (18/25 [72%]), CD3 (10/25 [40%]), and CD5 and CD7 (8/25 [32%] each). CD45 was expressed in 23 of 25 cases and CD13 in 7 of 9. Of 21 cases, 13 were anaplastic lymphoma kinase (ALK)+, all of which were CD4+, vs 5 of 8 ALK- cases (P = .042). Most ALCLs can be detected and characterized by multiparameter FC analysis. However, light scatter gating on typical lymphoid regions may yield false-negative results in a substantial number of cases.
KW - ALK
KW - Anaplastic large cell lymphoma
KW - Anaplastic lymphoma kinase
KW - Flow cytometry
UR - http://www.scopus.com/inward/record.url?scp=34547886159&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547886159&partnerID=8YFLogxK
U2 - 10.1309/GUHKGAJEJ72CEAL7
DO - 10.1309/GUHKGAJEJ72CEAL7
M3 - Article
C2 - 17638668
AN - SCOPUS:34547886159
SN - 0002-9173
VL - 128
SP - 314
EP - 322
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 2
ER -