Assessing the biological activity of the glucan phosphatase laforin

Carlos Romá-Mateo; Madushi Raththagala; Mathew S. Gentry; Pascual Sanz

doi:10.1007/978-1-4939-3746-2_7

Assessing the biological activity of the glucan phosphatase laforin

Carlos Romá-Mateo
, Madushi Raththagala
, Mathew S. Gentry
, Pascual Sanz

Producción científica: Chapter › revisión exhaustiva

1 Cita (Scopus)

Resumen

Glucan phosphatases are a recently discovered family of enzymes that dephosphorylate either starch or glycogen and are essential for proper starch metabolism in plants and glycogen metabolism in humans. Mutations in the gene encoding the only human glucan phosphatase, laforin, result in the fatal, neurodegenerative, epilepsy known as Lafora disease. Here, we describe phosphatase assays to assess both generic laforin phosphatase activity and laforin’s unique glycogen phosphatase activity.

Idioma original	English
Título de la publicación alojada	Methods in Molecular Biology
Páginas	107-119
Número de páginas	13
DOI	https://doi.org/10.1007/978-1-4939-3746-2_7
Estado	Published - 2016

Serie de la publicación

Nombre	Methods in Molecular Biology
Volumen	1447
ISSN (versión impresa)	1064-3745

Nota bibliográfica

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Financiación

This study was supported by grants from the Spanish Ministry of Education and Science SAF2014-54604-C3-1-R (P.S.), Generalitat Valenciana (PrometeoII/2014/029) (P.S.), NIH Grants R01NS070899 (M.S.G.), P20GM103486 (M.S.G.), Mizutani Foundation for Glycoscience Award (M.S.G.), and NSF Grant IIA-1355438 (M.S.G.). C.R.-M. was supported by the Saving Lives at Birth Consortium.

Financiadores	Número del financiador
National Science Foundation (NSF)	IIA-1355438
National Institutes of Health (NIH)	R01NS070899
National Institute of General Medical Sciences	P20GM103486
Mizutani Foundation for Glycoscience
Ministerio de Educación, Cultura y Deporte	SAF2014-54604-C3-1-R
Generalitat Valenciana	PrometeoII/2014/029

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Molecular Biology
Genetics

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keywords = "Dual-specificity phosphatase, Glycogen, Lafora disease",

author = "Carlos Rom{\'a}-Mateo and Madushi Raththagala and Gentry, \{Mathew S.\} and Pascual Sanz",

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Assessing the biological activity of the glucan phosphatase laforin

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Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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