Avian peritoneal exudate cells: A comparison of stimulation protocols

The influence of different stimulation protocols on the induction of peritoneal exudate cells (PECs) and adherent cells, and on the percentage of different adherent cell types was examined in the chicken and Japanese quail. The results suggest that different protocols may be selected to maximize isolation of specific PEC populations for immunological studies. In the chicken, starch, peptone, glycogen, and Sephadex G-40 were all equally effective and superior to saline in generating PECs. While a single injection of Sephadex produced the highest yield of adherent cells with a maximum percentage of macrophages, repeated injections of Sephadex led to dramatic increases in non-adherent PECs (lymphocytes). In contrast, a single injection of starch was optimum for generating non-macrophage adherent cells (primarily heterophils). Since responses of the Japanese quail to stimulation with starch and saline were similar to those observed for the chicken, it is suggested that these protocols may be generally applicable for use with avian species.