Blocking mitochondrial permeability transition prevents p53 mitochondrial translocation during skin tumor promotion

Jianfeng Liu; Daret K. St. Clair; Xin Gu; Yunfeng Zhao

doi:10.1016/j.febslet.2008.03.013

Blocking mitochondrial permeability transition prevents p53 mitochondrial translocation during skin tumor promotion

Jianfeng Liu
, Daret K. St. Clair
, Xin Gu
, Yunfeng Zhao

Producción científica: Article › revisión exhaustiva

32 Citas (Scopus)

Resumen

The tumor suppressor p53 can translocate into mitochondria and activate apoptosis. Here we studied whether p53 mitochondrial translocation and subsequent apoptosis were affected by blocking mitochondrial permeability transition pore using cyclosporine A (CsA) and bongkrekic acid (BA) in skin epidermal JB6 cells and skin tissues. Our results demonstrated that CsA and BA blocked TPA-induced p53 translocation, leading to protection against the loss of mitochondrial membrane potential and Complex I activity, and eventually suppression of apoptosis. Thus, our results suggest that mitochondrial permeability transition is required for p53 mitochondrial translocation.

Idioma original	English
Páginas (desde-hasta)	1319-1324
Número de páginas	6
Publicación	FEBS Letters
Volumen	582
N.º	9
DOI	https://doi.org/10.1016/j.febslet.2008.03.013
Estado	Published - abr 16 2008

Nota bibliográfica

Funding Information:
The authors wish to thank Dr. Nancy Colburn at NCI/NIH for providing us the mouse skin epidermal JB6 cells; Kathleen Llorens at LSU Health Sciences Center-Shreveport Research Core Facility for technical support in laser confocal experiments; Dr. Steven Pruett at LSUHSC-S for technical support on VersaDoc Imagine System; Dr. Tammy Dugas, Dr. Kenneth McMartin, Dr. Heather Kleiner, Bo Jiang, and Yan Li at LSUHSC-S for their technical supports. This work was supported in part by Grant Number R03CA128077 from the National Cancer Institute.

Financiación

The authors wish to thank Dr. Nancy Colburn at NCI/NIH for providing us the mouse skin epidermal JB6 cells; Kathleen Llorens at LSU Health Sciences Center-Shreveport Research Core Facility for technical support in laser confocal experiments; Dr. Steven Pruett at LSUHSC-S for technical support on VersaDoc Imagine System; Dr. Tammy Dugas, Dr. Kenneth McMartin, Dr. Heather Kleiner, Bo Jiang, and Yan Li at LSUHSC-S for their technical supports. This work was supported in part by Grant Number R03CA128077 from the National Cancer Institute.

Financiadores	Número del financiador
National Childhood Cancer Registry – National Cancer Institute	R03CA128077

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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@article{e9d4b65a86014f5d86c3d1d9b0d12926,

title = "Blocking mitochondrial permeability transition prevents p53 mitochondrial translocation during skin tumor promotion",

abstract = "The tumor suppressor p53 can translocate into mitochondria and activate apoptosis. Here we studied whether p53 mitochondrial translocation and subsequent apoptosis were affected by blocking mitochondrial permeability transition pore using cyclosporine A (CsA) and bongkrekic acid (BA) in skin epidermal JB6 cells and skin tissues. Our results demonstrated that CsA and BA blocked TPA-induced p53 translocation, leading to protection against the loss of mitochondrial membrane potential and Complex I activity, and eventually suppression of apoptosis. Thus, our results suggest that mitochondrial permeability transition is required for p53 mitochondrial translocation.",

keywords = "Apoptosis, Mitochondria, p53",

author = "Jianfeng Liu and \{St. Clair\}, \{Daret K.\} and Xin Gu and Yunfeng Zhao",

note = "Funding Information: The authors wish to thank Dr. Nancy Colburn at NCI/NIH for providing us the mouse skin epidermal JB6 cells; Kathleen Llorens at LSU Health Sciences Center-Shreveport Research Core Facility for technical support in laser confocal experiments; Dr. Steven Pruett at LSUHSC-S for technical support on VersaDoc Imagine System; Dr. Tammy Dugas, Dr. Kenneth McMartin, Dr. Heather Kleiner, Bo Jiang, and Yan Li at LSUHSC-S for their technical supports. This work was supported in part by Grant Number R03CA128077 from the National Cancer Institute.",

year = "2008",

month = apr,

day = "16",

doi = "10.1016/j.febslet.2008.03.013",

language = "English",

volume = "582",

pages = "1319--1324",

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TY - JOUR

T1 - Blocking mitochondrial permeability transition prevents p53 mitochondrial translocation during skin tumor promotion

AU - Liu, Jianfeng

AU - St. Clair, Daret K.

AU - Gu, Xin

AU - Zhao, Yunfeng

N1 - Funding Information: The authors wish to thank Dr. Nancy Colburn at NCI/NIH for providing us the mouse skin epidermal JB6 cells; Kathleen Llorens at LSU Health Sciences Center-Shreveport Research Core Facility for technical support in laser confocal experiments; Dr. Steven Pruett at LSUHSC-S for technical support on VersaDoc Imagine System; Dr. Tammy Dugas, Dr. Kenneth McMartin, Dr. Heather Kleiner, Bo Jiang, and Yan Li at LSUHSC-S for their technical supports. This work was supported in part by Grant Number R03CA128077 from the National Cancer Institute.

PY - 2008/4/16

Y1 - 2008/4/16

N2 - The tumor suppressor p53 can translocate into mitochondria and activate apoptosis. Here we studied whether p53 mitochondrial translocation and subsequent apoptosis were affected by blocking mitochondrial permeability transition pore using cyclosporine A (CsA) and bongkrekic acid (BA) in skin epidermal JB6 cells and skin tissues. Our results demonstrated that CsA and BA blocked TPA-induced p53 translocation, leading to protection against the loss of mitochondrial membrane potential and Complex I activity, and eventually suppression of apoptosis. Thus, our results suggest that mitochondrial permeability transition is required for p53 mitochondrial translocation.

AB - The tumor suppressor p53 can translocate into mitochondria and activate apoptosis. Here we studied whether p53 mitochondrial translocation and subsequent apoptosis were affected by blocking mitochondrial permeability transition pore using cyclosporine A (CsA) and bongkrekic acid (BA) in skin epidermal JB6 cells and skin tissues. Our results demonstrated that CsA and BA blocked TPA-induced p53 translocation, leading to protection against the loss of mitochondrial membrane potential and Complex I activity, and eventually suppression of apoptosis. Thus, our results suggest that mitochondrial permeability transition is required for p53 mitochondrial translocation.

KW - Apoptosis

KW - Mitochondria

KW - p53

UR - https://www.scopus.com/pages/publications/41449100068

UR - https://www.scopus.com/pages/publications/41449100068#tab=citedBy

U2 - 10.1016/j.febslet.2008.03.013

DO - 10.1016/j.febslet.2008.03.013

M3 - Article

C2 - 18358838

AN - SCOPUS:41449100068

SN - 0014-5793

VL - 582

SP - 1319

EP - 1324

JO - FEBS Letters

JF - FEBS Letters

IS - 9

ER -

Blocking mitochondrial permeability transition prevents p53 mitochondrial translocation during skin tumor promotion

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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