Borrelia burgdorferi SpoVG DNA- and RNA-binding protein modulates the physiology of the Lyme disease spirochete

Christina R. Savage; Brandon L. Jutras; Aaron Bestor; Kit Tilly; Patricia A. Rosa; Yvonne Tourand; Philip E. Stewart; Catherine A. Brissette; Brian Stevensona

doi:10.1128/JB.00033-18

Borrelia burgdorferi SpoVG DNA- and RNA-binding protein modulates the physiology of the Lyme disease spirochete

Christina R. Savage
, Brandon L. Jutras
, Aaron Bestor
, Kit Tilly
, Patricia A. Rosa
, Yvonne Tourand
, Philip E. Stewart
, Catherine A. Brissette
, Brian Stevensona

Producción científica: Article › revisión exhaustiva

23 Citas (Scopus)

Resumen

The SpoVG protein of Borrelia burgdorferi, the Lyme disease spirochete, binds to specific sites of DNA and RNA. The bacterium regulates transcription of spoVG during the natural tick-mammal infectious cycle and in response to some changes in culture conditions. Bacterial levels of spoVG mRNA and SpoVG protein did not necessarily correlate, suggesting that posttranscriptional mechanisms also control protein levels. Consistent with this, SpoVG binds to its own mRNA, adjacent to the ribosome-binding site. SpoVG also binds to two DNA sites in the glpFKD operon and to two RNA sites in glpFKD mRNA; that operon encodes genes necessary for glycerol catabolism and is important for colonization in ticks. In addition, spirochetes engineered to dysregulate spoVG exhibited physiological alterations.

Idioma original	English
Número de artículo	e00033.18
Publicación	Journal of Bacteriology
Volumen	200
N.º	12
DOI	https://doi.org/10.1128/JB.00033-18
Estado	Published - jun 1 2018

Nota bibliográfica

Publisher Copyright:
© 2018 American Society for Microbiology.

Financiación

This work was supported by a grant from the National Research Fund for Tick-Borne Diseases to B.S., National Institutes of Health grant P20GM113123 to C.A.B., and funds from the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health to P.A.R. This work was supported by a grant from the National Research Fund for Tick-Borne Diseases to B.S., National Institutes of Health grant P20GM113123 to C.A.B., and funds from the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health to P.A.R. The funders had no role in study design, data collection and analysis, the decision to publish, or preparation of the manuscript. We thank Will Arnold and Kathryn Lethbridge for helpful discussions during these studies

Financiadores	Número del financiador
Division of Intramural Research
National Research Fund for Tick Borne Diseases Inc
National Institutes of Health (NIH)
National Institute of General Medical Sciences DP2GM119177 Sophie Dumont National Institute of General Medical Sciences	P20GM113123
National Institute of Allergy and Infectious Diseases
Division of Intramural Research, National Institute of Allergy and Infectious Diseases

ASJC Scopus subject areas

Microbiology
Molecular Biology

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title = "Borrelia burgdorferi SpoVG DNA- and RNA-binding protein modulates the physiology of the Lyme disease spirochete",

abstract = "The SpoVG protein of Borrelia burgdorferi, the Lyme disease spirochete, binds to specific sites of DNA and RNA. The bacterium regulates transcription of spoVG during the natural tick-mammal infectious cycle and in response to some changes in culture conditions. Bacterial levels of spoVG mRNA and SpoVG protein did not necessarily correlate, suggesting that posttranscriptional mechanisms also control protein levels. Consistent with this, SpoVG binds to its own mRNA, adjacent to the ribosome-binding site. SpoVG also binds to two DNA sites in the glpFKD operon and to two RNA sites in glpFKD mRNA; that operon encodes genes necessary for glycerol catabolism and is important for colonization in ticks. In addition, spirochetes engineered to dysregulate spoVG exhibited physiological alterations.",

keywords = "Borrelia, DNA-binding proteins, RNA-binding proteins, Regulation",

author = "Savage, \{Christina R.\} and Jutras, \{Brandon L.\} and Aaron Bestor and Kit Tilly and Rosa, \{Patricia A.\} and Yvonne Tourand and Stewart, \{Philip E.\} and Brissette, \{Catherine A.\} and Brian Stevensona",

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doi = "10.1128/JB.00033-18",

language = "English",

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T1 - Borrelia burgdorferi SpoVG DNA- and RNA-binding protein modulates the physiology of the Lyme disease spirochete

AU - Savage, Christina R.

AU - Jutras, Brandon L.

AU - Bestor, Aaron

AU - Tilly, Kit

AU - Rosa, Patricia A.

AU - Tourand, Yvonne

AU - Stewart, Philip E.

AU - Brissette, Catherine A.

AU - Stevensona, Brian

PY - 2018/6/1

Y1 - 2018/6/1

N2 - The SpoVG protein of Borrelia burgdorferi, the Lyme disease spirochete, binds to specific sites of DNA and RNA. The bacterium regulates transcription of spoVG during the natural tick-mammal infectious cycle and in response to some changes in culture conditions. Bacterial levels of spoVG mRNA and SpoVG protein did not necessarily correlate, suggesting that posttranscriptional mechanisms also control protein levels. Consistent with this, SpoVG binds to its own mRNA, adjacent to the ribosome-binding site. SpoVG also binds to two DNA sites in the glpFKD operon and to two RNA sites in glpFKD mRNA; that operon encodes genes necessary for glycerol catabolism and is important for colonization in ticks. In addition, spirochetes engineered to dysregulate spoVG exhibited physiological alterations.

AB - The SpoVG protein of Borrelia burgdorferi, the Lyme disease spirochete, binds to specific sites of DNA and RNA. The bacterium regulates transcription of spoVG during the natural tick-mammal infectious cycle and in response to some changes in culture conditions. Bacterial levels of spoVG mRNA and SpoVG protein did not necessarily correlate, suggesting that posttranscriptional mechanisms also control protein levels. Consistent with this, SpoVG binds to its own mRNA, adjacent to the ribosome-binding site. SpoVG also binds to two DNA sites in the glpFKD operon and to two RNA sites in glpFKD mRNA; that operon encodes genes necessary for glycerol catabolism and is important for colonization in ticks. In addition, spirochetes engineered to dysregulate spoVG exhibited physiological alterations.

KW - Borrelia

KW - DNA-binding proteins

KW - RNA-binding proteins

KW - Regulation

UR - https://www.scopus.com/pages/publications/85047494010

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AN - SCOPUS:85047494010

SN - 0021-9193

VL - 200

JO - Journal of Bacteriology

JF - Journal of Bacteriology

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ER -

Borrelia burgdorferi SpoVG DNA- and RNA-binding protein modulates the physiology of the Lyme disease spirochete

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Nota bibliográfica

Financiación

ASJC Scopus subject areas

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