Ceramide and S1P signaling in embryonic stem cell differentiation

Guanghu Wang; Stefka D. Spassieva; Erhard Bieberich

doi:10.1007/7651_2017_43

Ceramide and S1P signaling in embryonic stem cell differentiation

Physiology

Producción científica: Chapter › revisión exhaustiva

21 Citas (Scopus)

Resumen

Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term “morphogenetic lipids” for sphingolipids that regulate stem cells during embryonic and postnatal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.

Idioma original	English
Título de la publicación alojada	Methods in Molecular Biology
Páginas	153-171
Número de páginas	19
DOI	https://doi.org/10.1007/7651_2017_43
Estado	Published - 2018

Serie de la publicación

Nombre	Methods in Molecular Biology
Volumen	1697
ISSN (versión impresa)	1064-3745

Nota bibliográfica

Publisher Copyright:
© 2017, Springer Science+Business Media New York.

Financiación

This study was in part supported by the grants R01NS046835, R01AG034389, R01NS095215, and NSF grant 1615874.

Financiadores	Número del financiador
National Science Foundation Arctic Social Science Program	R01NS095215, R01AG034389
National Science Foundation Arctic Social Science Program
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council	R01NS046835
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council

ASJC Scopus subject areas

Molecular Biology
Genetics

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abstract = "Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term “morphogenetic lipids” for sphingolipids that regulate stem cells during embryonic and postnatal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.",

keywords = "Apoptosis, Ceramide, Neuroprogenitor, Oligodendrocyte precursor, Sphingolipid, Sphingosine-1-phosphate, Teratoma",

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AU - Bieberich, Erhard

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AB - Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term “morphogenetic lipids” for sphingolipids that regulate stem cells during embryonic and postnatal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.

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KW - Ceramide

KW - Neuroprogenitor

KW - Oligodendrocyte precursor

KW - Sphingolipid

KW - Sphingosine-1-phosphate

KW - Teratoma

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Ceramide and S1P signaling in embryonic stem cell differentiation

Resumen

Serie de la publicación

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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