Characterization of a new myeloid leukemia cell line with normal cytogenetics (CG-SH)

Reinhold Munker; Mary Lowery Nordberg; Diana Veillon; B. Jill Williams; Anthony Roggero; Wolfgang Kern; Frank Dicker; Torsten Haferlach

doi:10.1016/j.leukres.2009.04.008

Characterization of a new myeloid leukemia cell line with normal cytogenetics (CG-SH)

Reinhold Munker
, Mary Lowery Nordberg
, Diana Veillon
, B. Jill Williams
, Anthony Roggero
, Wolfgang Kern
, Frank Dicker
, Torsten Haferlach

Producción científica: Article › revisión exhaustiva

9 Citas (Scopus)

Resumen

A new myeloid leukemia cell line (CG-SH) with normal cytogenetics was established from a patient with acute myelogenous leukemia (AML) following myelodysplastic syndrome (MDS). The cells of CG-SH are immature blasts and have an immature myeloid phenotype (positive for myeloperoxidase, CD7, CD34, CD38, CD117, HLA-DR, negative for CD10, CD19, CD20, CD41, CD42). A partial expression of CD13, CD15, CD65 and a weak expression of CD33 and CD133 was noted. The cells are negative for EBER. By molecular analysis, a mutation of NRAS and heterozygous mutations of RUNX1 were detected. No mutations were detected in FLT3-ITD, MLL-PTD or NPM1. By real-time PCR, a series of 19 microRNAs was identified which are strongly expressed in CG-SH. In conclusion, a new cell line was established which will be useful for the study of AML with normal cytogenetics and mutations in NRAS and/or RUNX1.

Idioma original	English
Páginas (desde-hasta)	1405-1408
Número de páginas	4
Publicación	Leukemia Research
Volumen	33
N.º	10
DOI	https://doi.org/10.1016/j.leukres.2009.04.008
Estado	Published - oct 2009

Financiación

The authors thank Dr. Kevin Dowden for a photomicrograph, Cory (Joseph) Cordova and Paula Polk for technical assistance, the Feist Weiller Cancer Center for financial support and Drs. Shari Meyers and Jonathan Glass for critical comments.

Financiadores
LSUHSC-Feist Weiller Cancer Center, Shreveport

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/j.leukres.2009.04.008

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title = "Characterization of a new myeloid leukemia cell line with normal cytogenetics (CG-SH)",

abstract = "A new myeloid leukemia cell line (CG-SH) with normal cytogenetics was established from a patient with acute myelogenous leukemia (AML) following myelodysplastic syndrome (MDS). The cells of CG-SH are immature blasts and have an immature myeloid phenotype (positive for myeloperoxidase, CD7, CD34, CD38, CD117, HLA-DR, negative for CD10, CD19, CD20, CD41, CD42). A partial expression of CD13, CD15, CD65 and a weak expression of CD33 and CD133 was noted. The cells are negative for EBER. By molecular analysis, a mutation of NRAS and heterozygous mutations of RUNX1 were detected. No mutations were detected in FLT3-ITD, MLL-PTD or NPM1. By real-time PCR, a series of 19 microRNAs was identified which are strongly expressed in CG-SH. In conclusion, a new cell line was established which will be useful for the study of AML with normal cytogenetics and mutations in NRAS and/or RUNX1.",

keywords = "AML, Cell line, Normal cytogenetics",

author = "Reinhold Munker and Nordberg, \{Mary Lowery\} and Diana Veillon and Williams, \{B. Jill\} and Anthony Roggero and Wolfgang Kern and Frank Dicker and Torsten Haferlach",

year = "2009",

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doi = "10.1016/j.leukres.2009.04.008",

language = "English",

volume = "33",

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T1 - Characterization of a new myeloid leukemia cell line with normal cytogenetics (CG-SH)

AU - Munker, Reinhold

AU - Nordberg, Mary Lowery

AU - Veillon, Diana

AU - Williams, B. Jill

AU - Roggero, Anthony

AU - Kern, Wolfgang

AU - Dicker, Frank

AU - Haferlach, Torsten

PY - 2009/10

Y1 - 2009/10

N2 - A new myeloid leukemia cell line (CG-SH) with normal cytogenetics was established from a patient with acute myelogenous leukemia (AML) following myelodysplastic syndrome (MDS). The cells of CG-SH are immature blasts and have an immature myeloid phenotype (positive for myeloperoxidase, CD7, CD34, CD38, CD117, HLA-DR, negative for CD10, CD19, CD20, CD41, CD42). A partial expression of CD13, CD15, CD65 and a weak expression of CD33 and CD133 was noted. The cells are negative for EBER. By molecular analysis, a mutation of NRAS and heterozygous mutations of RUNX1 were detected. No mutations were detected in FLT3-ITD, MLL-PTD or NPM1. By real-time PCR, a series of 19 microRNAs was identified which are strongly expressed in CG-SH. In conclusion, a new cell line was established which will be useful for the study of AML with normal cytogenetics and mutations in NRAS and/or RUNX1.

AB - A new myeloid leukemia cell line (CG-SH) with normal cytogenetics was established from a patient with acute myelogenous leukemia (AML) following myelodysplastic syndrome (MDS). The cells of CG-SH are immature blasts and have an immature myeloid phenotype (positive for myeloperoxidase, CD7, CD34, CD38, CD117, HLA-DR, negative for CD10, CD19, CD20, CD41, CD42). A partial expression of CD13, CD15, CD65 and a weak expression of CD33 and CD133 was noted. The cells are negative for EBER. By molecular analysis, a mutation of NRAS and heterozygous mutations of RUNX1 were detected. No mutations were detected in FLT3-ITD, MLL-PTD or NPM1. By real-time PCR, a series of 19 microRNAs was identified which are strongly expressed in CG-SH. In conclusion, a new cell line was established which will be useful for the study of AML with normal cytogenetics and mutations in NRAS and/or RUNX1.

KW - AML

KW - Cell line

KW - Normal cytogenetics

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U2 - 10.1016/j.leukres.2009.04.008

DO - 10.1016/j.leukres.2009.04.008

M3 - Article

C2 - 19414191

AN - SCOPUS:67649311629

SN - 0145-2126

VL - 33

SP - 1405

EP - 1408

JO - Leukemia Research

JF - Leukemia Research

IS - 10

ER -

Characterization of a new myeloid leukemia cell line with normal cytogenetics (CG-SH)

Resumen

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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