Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue

Michael N. Zook; Joseph Chappell; Joseph A. Kúc

doi:10.1016/0031-9422(92)83703-2

Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue

Michael N. Zook
, Joseph Chappell
, Joseph A. Kúc

Producción científica: Article › revisión exhaustiva

16 Citas (Scopus)

Resumen

The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The K_m of the potato sesquiterpene cyclase for farnesyl diphosphate was 7μM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID₅₀ of approximately 4 mM.

Idioma original	English
Páginas (desde-hasta)	3441-3445
Número de páginas	5
Publicación	Phytochemistry
Volumen	31
N.º	10
DOI	https://doi.org/10.1016/0031-9422(92)83703-2
Estado	Published - oct 1992

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

Acceder al documento

10.1016/0031-9422(92)83703-2

Otros archivos y enlaces

Citar esto

@article{efb2aa6b0b364e17b57fa1888504b439,

title = "Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue",

abstract = "The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The Km of the potato sesquiterpene cyclase for farnesyl diphosphate was 7μM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID50 of approximately 4 mM.",

keywords = "5-epi-aristolochene, Solanaceae, Solanum tuberosum, biosynthesis, rishitin, sesquiterpene cyclase., sesquiterpenoids",

author = "Zook, \{Michael N.\} and Joseph Chappell and K{\'u}c, \{Joseph A.\}",

year = "1992",

month = oct,

doi = "10.1016/0031-9422(92)83703-2",

language = "English",

volume = "31",

pages = "3441--3445",

number = "10",

}

TY - JOUR

T1 - Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue

AU - Zook, Michael N.

AU - Chappell, Joseph

AU - Kúc, Joseph A.

PY - 1992/10

Y1 - 1992/10

N2 - The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The Km of the potato sesquiterpene cyclase for farnesyl diphosphate was 7μM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID50 of approximately 4 mM.

AB - The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The Km of the potato sesquiterpene cyclase for farnesyl diphosphate was 7μM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID50 of approximately 4 mM.

KW - 5-epi-aristolochene

KW - Solanaceae

KW - Solanum tuberosum

KW - biosynthesis

KW - rishitin

KW - sesquiterpene cyclase.

KW - sesquiterpenoids

UR - https://www.scopus.com/pages/publications/0000702743

UR - https://www.scopus.com/pages/publications/0000702743#tab=citedBy

U2 - 10.1016/0031-9422(92)83703-2

DO - 10.1016/0031-9422(92)83703-2

M3 - Article

AN - SCOPUS:0000702743

SN - 0031-9422

VL - 31

SP - 3441

EP - 3445

JO - Phytochemistry

JF - Phytochemistry

IS - 10

ER -

Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue

Resumen

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto