Characterization of microsatellite loci from the Malagasy endemic, Tina striata Radlk. (Sapindaceae)

Laura B. Vary; Stacey L. Lance; Cris Hagen; Olga Tsyusko; Travis C. Glenn; Ann K. Sakai; Stephen G. Weller

doi:10.1007/s10592-008-9721-z

Characterization of microsatellite loci from the Malagasy endemic, Tina striata Radlk. (Sapindaceae)

Laura B. Vary, Stacey L. Lance, Cris Hagen, Olga Tsyusko, Travis C. Glenn, Ann K. Sakai, Stephen G. Weller

Producción científica: Article › revisión exhaustiva

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Resumen

We isolated and characterized 12 polymorphic microsatellite loci in the Malagasy endemic, Tina striata Radlk. (Sapindaceae). The number of alleles per locus ranged from 2 to 6 (N = 28 individuals). Polymorphic information content ranged from 0.132 to 0.767 and observed heterozygosity from 0.154 to 0.800. These markers will be valuable in estimating outcrossing rates and genetic variation in populations of T. striata, and aid in the conservation of populations of T. striata and other closely related species in the Malagasy Sapindaceae.

Idioma original	English
Páginas (desde-hasta)	1113-1115
Número de páginas	3
Publicación	Conservation Genetics
Volumen	10
N.º	4
DOI	https://doi.org/10.1007/s10592-008-9721-z
Estado	Published - ago 2009

Nota bibliográfica

Funding Information:
Acknowledgments This work was funded in part by a National Science Foundation Graduate Research Fellowship awarded to LBV, and funds from the University of California–Irvine to AKS. We thank Sven Buerki for supplying additional DNA material, Felipe Barreto and Steve Lockton for their advice and help in the lab, Brandon Gaut for use of lab material and equipment, and Lalao Andriamahefarivo, Patrice Antilahimena, the staff of the Missouri Botanical Garden’s Madagascar Office and the staff of the Parc Botanique et Zoologique de Tsimbazaza for help with permits and fieldwork in Madagascar.

Financiación

Acknowledgments This work was funded in part by a National Science Foundation Graduate Research Fellowship awarded to LBV, and funds from the University of California–Irvine to AKS. We thank Sven Buerki for supplying additional DNA material, Felipe Barreto and Steve Lockton for their advice and help in the lab, Brandon Gaut for use of lab material and equipment, and Lalao Andriamahefarivo, Patrice Antilahimena, the staff of the Missouri Botanical Garden’s Madagascar Office and the staff of the Parc Botanique et Zoologique de Tsimbazaza for help with permits and fieldwork in Madagascar.

Financiadores	Número del financiador
University of California Irvine
National Science Foundation (NSF)

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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title = "Characterization of microsatellite loci from the Malagasy endemic, Tina striata Radlk. (Sapindaceae)",

abstract = "We isolated and characterized 12 polymorphic microsatellite loci in the Malagasy endemic, Tina striata Radlk. (Sapindaceae). The number of alleles per locus ranged from 2 to 6 (N = 28 individuals). Polymorphic information content ranged from 0.132 to 0.767 and observed heterozygosity from 0.154 to 0.800. These markers will be valuable in estimating outcrossing rates and genetic variation in populations of T. striata, and aid in the conservation of populations of T. striata and other closely related species in the Malagasy Sapindaceae.",

keywords = "Dioecy, Madagascar, Microsatellites, Monoecy, Outcrossing, Sapindaceae, Tina striata",

author = "Vary, \{Laura B.\} and Lance, \{Stacey L.\} and Cris Hagen and Olga Tsyusko and Glenn, \{Travis C.\} and Sakai, \{Ann K.\} and Weller, \{Stephen G.\}",

note = "Funding Information: Acknowledgments This work was funded in part by a National Science Foundation Graduate Research Fellowship awarded to LBV, and funds from the University of California–Irvine to AKS. We thank Sven Buerki for supplying additional DNA material, Felipe Barreto and Steve Lockton for their advice and help in the lab, Brandon Gaut for use of lab material and equipment, and Lalao Andriamahefarivo, Patrice Antilahimena, the staff of the Missouri Botanical Garden{\textquoteright}s Madagascar Office and the staff of the Parc Botanique et Zoologique de Tsimbazaza for help with permits and fieldwork in Madagascar.",

year = "2009",

month = aug,

doi = "10.1007/s10592-008-9721-z",

language = "English",

volume = "10",

pages = "1113--1115",

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AU - Vary, Laura B.

AU - Lance, Stacey L.

AU - Hagen, Cris

AU - Tsyusko, Olga

AU - Glenn, Travis C.

AU - Sakai, Ann K.

AU - Weller, Stephen G.

N1 - Funding Information: Acknowledgments This work was funded in part by a National Science Foundation Graduate Research Fellowship awarded to LBV, and funds from the University of California–Irvine to AKS. We thank Sven Buerki for supplying additional DNA material, Felipe Barreto and Steve Lockton for their advice and help in the lab, Brandon Gaut for use of lab material and equipment, and Lalao Andriamahefarivo, Patrice Antilahimena, the staff of the Missouri Botanical Garden’s Madagascar Office and the staff of the Parc Botanique et Zoologique de Tsimbazaza for help with permits and fieldwork in Madagascar.

PY - 2009/8

Y1 - 2009/8

N2 - We isolated and characterized 12 polymorphic microsatellite loci in the Malagasy endemic, Tina striata Radlk. (Sapindaceae). The number of alleles per locus ranged from 2 to 6 (N = 28 individuals). Polymorphic information content ranged from 0.132 to 0.767 and observed heterozygosity from 0.154 to 0.800. These markers will be valuable in estimating outcrossing rates and genetic variation in populations of T. striata, and aid in the conservation of populations of T. striata and other closely related species in the Malagasy Sapindaceae.

AB - We isolated and characterized 12 polymorphic microsatellite loci in the Malagasy endemic, Tina striata Radlk. (Sapindaceae). The number of alleles per locus ranged from 2 to 6 (N = 28 individuals). Polymorphic information content ranged from 0.132 to 0.767 and observed heterozygosity from 0.154 to 0.800. These markers will be valuable in estimating outcrossing rates and genetic variation in populations of T. striata, and aid in the conservation of populations of T. striata and other closely related species in the Malagasy Sapindaceae.

KW - Dioecy

KW - Madagascar

KW - Microsatellites

KW - Monoecy

KW - Outcrossing

KW - Sapindaceae

KW - Tina striata

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U2 - 10.1007/s10592-008-9721-z

DO - 10.1007/s10592-008-9721-z

M3 - Article

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SN - 1566-0621

VL - 10

SP - 1113

EP - 1115

JO - Conservation Genetics

JF - Conservation Genetics

IS - 4

ER -

Characterization of microsatellite loci from the Malagasy endemic, Tina striata Radlk. (Sapindaceae)

Resumen

Nota bibliográfica

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ASJC Scopus subject areas

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