Coevolutionary information, protein folding landscapes, and the thermodynamics of natural selection

The energy landscape used by nature over evolutionary timescales to select protein sequences is essentially the same as the one that folds these sequences into functioning proteins, sometimes in microseconds. Weshow that genomic data, physical coarse-grained free energy functions, and family-specific information theoretic models can be combined to give consistent estimates of energy landscape characteristics of natural proteins. One such characteristic is the effective temperature T_sel at which these foldable sequences have been selected in sequence space by evolution. T_sel quantifies the importance of folded-state energetics and structural specificity for molecular evolution. Across all protein families studied, our estimates for T _sel are well below the experimental folding temperatures, indicating that the energy landscapes of natural foldable proteins are strongly funneled toward the native state.