Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Olga V. Chumakova; Anton V. Liopo; Valery G. Andreev; Inga Cicenaite; B. Mark Evers; Shilla Chakrabarty; Todd C. Pappas; Rinat O. Esenaliev

doi:10.1016/j.canlet.2007.11.023

Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Olga V. Chumakova
, Anton V. Liopo
, Valery G. Andreev
, Inga Cicenaite
, B. Mark Evers
, Shilla Chakrabarty
, Todd C. Pappas
, Rinat O. Esenaliev

Producción científica: Article › revisión exhaustiva

141 Citas (Scopus)

Resumen

The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.

Idioma original	English
Páginas (desde-hasta)	215-225
Número de páginas	11
Publicación	Cancer Letters
Volumen	261
N.º	2
DOI	https://doi.org/10.1016/j.canlet.2007.11.023
Estado	Published - mar 18 2008

Nota bibliográfica

Funding Information:
These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant #W81XWH-04-1-0247) and by the NIH Grant #R01CA104748.

Financiación

These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant #W81XWH-04-1-0247) and by the NIH Grant #R01CA104748.

Financiadores	Número del financiador
Department of Defense prostate cancer research program	81XWH-04-1-0247
National Institutes of Health (NIH)
National Childhood Cancer Registry – National Cancer Institute	R01CA104748

ASJC Scopus subject areas

Oncology
Cancer Research

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

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10.1016/j.canlet.2007.11.023

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@article{b1ce7a42a1d6429f989f21362a29b248,

title = "Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo",

abstract = "The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.",

keywords = "Gene delivery, PEI, PLGA nanoparticles, Tumor, Ultrasound",

author = "Chumakova, \{Olga V.\} and Liopo, \{Anton V.\} and Andreev, \{Valery G.\} and Inga Cicenaite and Evers, \{B. Mark\} and Shilla Chakrabarty and Pappas, \{Todd C.\} and Esenaliev, \{Rinat O.\}",

note = "Funding Information: These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant \#W81XWH-04-1-0247) and by the NIH Grant \#R01CA104748. ",

year = "2008",

month = mar,

day = "18",

doi = "10.1016/j.canlet.2007.11.023",

language = "English",

volume = "261",

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AU - Chumakova, Olga V.

AU - Liopo, Anton V.

AU - Andreev, Valery G.

AU - Cicenaite, Inga

AU - Evers, B. Mark

AU - Chakrabarty, Shilla

AU - Pappas, Todd C.

AU - Esenaliev, Rinat O.

N1 - Funding Information: These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant #W81XWH-04-1-0247) and by the NIH Grant #R01CA104748.

PY - 2008/3/18

Y1 - 2008/3/18

N2 - The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.

AB - The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.

KW - Gene delivery

KW - PEI

KW - PLGA nanoparticles

KW - Tumor

KW - Ultrasound

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DO - 10.1016/j.canlet.2007.11.023

M3 - Article

C2 - 18164806

AN - SCOPUS:39149137192

SN - 0304-3835

VL - 261

SP - 215

EP - 225

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

ODS de las Naciones Unidas

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