CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum

Jingjing Xu; Amit Roy; Subba Reddy Palli

doi:10.1038/s41598-018-19667-6

CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum

Jingjing Xu
, Amit Roy
, Subba Reddy Palli

Producción científica: Article › revisión exhaustiva

33 Citas (Scopus)

Resumen

Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both T. castaneum and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect, T.castaneum.

Idioma original	English
Número de artículo	1426
Publicación	Scientific Reports
Volumen	8
N.º	1
DOI	https://doi.org/10.1038/s41598-018-19667-6
Estado	Published - dic 1 2018

Nota bibliográfica

Publisher Copyright:
© 2018 The Author(s).

Financiación

The TcA cells are a gift from Dr. Goodman’s USDA lab, and this work was supported by National Institutes of Health Grant GM070559-11) and National Institute of Food and Agriculture, US Department of Agriculture, HATCH under 2351177000. This is Publication number 18-08-004 from the Kentucky Agricultural Experimental Station and published with the approval of the director. Authors also thank Prof.Fredrick Schylter, Department of Chemical Ecology, Swedish University of Agricultural Sciences, Alnarp, Sweden and Dr.Roman Modlinger, Faculty of Forestry and Wood Sciences, EXTEMIT-K, Czech University of Life Sciences, Czech Republic for their valuable inputs during the statistical analysis and interpretation of results.

Financiadores	Número del financiador
US Department of Agriculture	2351177000
National Institutes of Health (NIH)
National Institute of General Medical Sciences DP2GM119177 Sophie Dumont National Institute of General Medical Sciences	R01GM070559
U.S. Department of Agriculture
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative

ASJC Scopus subject areas

General

Acceder al documento

10.1038/s41598-018-19667-6

Otros archivos y enlaces

Citar esto

@article{2b9b9bd6b76e4f4abfa280cd9833f190,

title = "CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum",

abstract = "Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both T. castaneum and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect, T.castaneum.",

author = "Jingjing Xu and Amit Roy and Palli, \{Subba Reddy\}",

note = "Publisher Copyright: {\textcopyright} 2018 The Author(s).",

year = "2018",

month = dec,

day = "1",

doi = "10.1038/s41598-018-19667-6",

language = "English",

volume = "8",

number = "1",

}

TY - JOUR

T1 - CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum

AU - Xu, Jingjing

AU - Roy, Amit

AU - Palli, Subba Reddy

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both T. castaneum and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect, T.castaneum.

AB - Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both T. castaneum and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect, T.castaneum.

UR - https://www.scopus.com/pages/publications/85041068110

UR - https://www.scopus.com/pages/publications/85041068110#tab=citedBy

U2 - 10.1038/s41598-018-19667-6

DO - 10.1038/s41598-018-19667-6

M3 - Article

C2 - 29362416

AN - SCOPUS:85041068110

SN - 2045-2322

VL - 8

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 1426

ER -

CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto