Crystal Structure of the N-Terminal Domain of the Secretin GspD from ETEC Determined with the Assistance of a Nanobody

Konstantin V. Korotkov; Els Pardon; Jan Steyaert; Wim G.J. Hol

doi:10.1016/j.str.2008.11.011

Crystal Structure of the N-Terminal Domain of the Secretin GspD from ETEC Determined with the Assistance of a Nanobody

Konstantin V. Korotkov
, Els Pardon
, Jan Steyaert
, Wim G.J. Hol

Producción científica: Article › revisión exhaustiva

155 Citas (Scopus)

Resumen

Secretins are among the largest bacterial outer membrane proteins known. Here we report the crystal structure of the periplasmic N-terminal domain of GspD (peri-GspD) from the type 2 secretion system (T2SS) secretin in complex with a nanobody, the VHH domain of a heavy-chain camelid antibody. Two different crystal forms contained the same compact peri-GspD:nanobody heterotetramer. The nanobody contacts peri-GspD mainly via CDR3 and framework residues. The peri-GspD structure reveals three subdomains, with the second and third subdomains exhibiting the KH fold which also occurs in ring-forming proteins of the type 3 secretion system. The first subdomain of GspD is related to domains in phage tail proteins and outer membrane TonB-dependent receptors. A dodecameric peri-GspD model is proposed in which a solvent-accessible β strand of the first subdomain interacts with secreted proteins and/or T2SS partner proteins by β strand complementation.

Idioma original	English
Páginas (desde-hasta)	255-265
Número de páginas	11
Publicación	Structure
Volumen	17
N.º	2
DOI	https://doi.org/10.1016/j.str.2008.11.011
Estado	Published - feb 13 2009

Nota bibliográfica

Funding Information:
We thank Stephen Moseley from the Department of Microbiology, University of Washington, for providing the ETEC genomic DNA, and the support staff of beamline BL9-2 of the SSRL for assistance during data collection. Portions of this research were carried out at the Stanford Synchrotron Radiation Laboratory, supported by the Department of Energy and by the NIH. We thank Nele Buys for the selection, expression, and purification of the nanobodies. This study was supported by National Institutes of Health grant AI34501 (to W.G.J.H.) and by the Belgian Government under the framework of the Interuniversity Attraction Poles (I.A.P. P6/19).

Financiación

We thank Stephen Moseley from the Department of Microbiology, University of Washington, for providing the ETEC genomic DNA, and the support staff of beamline BL9-2 of the SSRL for assistance during data collection. Portions of this research were carried out at the Stanford Synchrotron Radiation Laboratory, supported by the Department of Energy and by the NIH. We thank Nele Buys for the selection, expression, and purification of the nanobodies. This study was supported by National Institutes of Health grant AI34501 (to W.G.J.H.) and by the Belgian Government under the framework of the Interuniversity Attraction Poles (I.A.P. P6/19).

Financiadores	Número del financiador
National Institutes of Health (NIH)	P6/19
U.S. Department of Energy EPSCoR
National Institute of Allergy and Infectious F32-AI286447 Cydney N. Johnson Diseases National Institute of Allergy and Infectious R01AI168214 Jason W. Rosch Diseases National Institute of Allergy and Infectious P30 Cydney N. Johnson Diseases National Institute of Allergy and Infectious R00-AI166116 Christopher D. Radka Diseases National Institute of Allergy and Infectious T32-AI106700 Cydney N. Johnson Diseases National Institute of Allergy and Infectious R01AI192221 Jason W. Rosch Diseases National Inst...	R56AI034501

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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title = "Crystal Structure of the N-Terminal Domain of the Secretin GspD from ETEC Determined with the Assistance of a Nanobody",

abstract = "Secretins are among the largest bacterial outer membrane proteins known. Here we report the crystal structure of the periplasmic N-terminal domain of GspD (peri-GspD) from the type 2 secretion system (T2SS) secretin in complex with a nanobody, the VHH domain of a heavy-chain camelid antibody. Two different crystal forms contained the same compact peri-GspD:nanobody heterotetramer. The nanobody contacts peri-GspD mainly via CDR3 and framework residues. The peri-GspD structure reveals three subdomains, with the second and third subdomains exhibiting the KH fold which also occurs in ring-forming proteins of the type 3 secretion system. The first subdomain of GspD is related to domains in phage tail proteins and outer membrane TonB-dependent receptors. A dodecameric peri-GspD model is proposed in which a solvent-accessible β strand of the first subdomain interacts with secreted proteins and/or T2SS partner proteins by β strand complementation.",

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note = "Funding Information: We thank Stephen Moseley from the Department of Microbiology, University of Washington, for providing the ETEC genomic DNA, and the support staff of beamline BL9-2 of the SSRL for assistance during data collection. Portions of this research were carried out at the Stanford Synchrotron Radiation Laboratory, supported by the Department of Energy and by the NIH. We thank Nele Buys for the selection, expression, and purification of the nanobodies. This study was supported by National Institutes of Health grant AI34501 (to W.G.J.H.) and by the Belgian Government under the framework of the Interuniversity Attraction Poles (I.A.P. P6/19). ",

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AU - Korotkov, Konstantin V.

AU - Pardon, Els

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AU - Hol, Wim G.J.

N1 - Funding Information: We thank Stephen Moseley from the Department of Microbiology, University of Washington, for providing the ETEC genomic DNA, and the support staff of beamline BL9-2 of the SSRL for assistance during data collection. Portions of this research were carried out at the Stanford Synchrotron Radiation Laboratory, supported by the Department of Energy and by the NIH. We thank Nele Buys for the selection, expression, and purification of the nanobodies. This study was supported by National Institutes of Health grant AI34501 (to W.G.J.H.) and by the Belgian Government under the framework of the Interuniversity Attraction Poles (I.A.P. P6/19).

PY - 2009/2/13

Y1 - 2009/2/13

N2 - Secretins are among the largest bacterial outer membrane proteins known. Here we report the crystal structure of the periplasmic N-terminal domain of GspD (peri-GspD) from the type 2 secretion system (T2SS) secretin in complex with a nanobody, the VHH domain of a heavy-chain camelid antibody. Two different crystal forms contained the same compact peri-GspD:nanobody heterotetramer. The nanobody contacts peri-GspD mainly via CDR3 and framework residues. The peri-GspD structure reveals three subdomains, with the second and third subdomains exhibiting the KH fold which also occurs in ring-forming proteins of the type 3 secretion system. The first subdomain of GspD is related to domains in phage tail proteins and outer membrane TonB-dependent receptors. A dodecameric peri-GspD model is proposed in which a solvent-accessible β strand of the first subdomain interacts with secreted proteins and/or T2SS partner proteins by β strand complementation.

AB - Secretins are among the largest bacterial outer membrane proteins known. Here we report the crystal structure of the periplasmic N-terminal domain of GspD (peri-GspD) from the type 2 secretion system (T2SS) secretin in complex with a nanobody, the VHH domain of a heavy-chain camelid antibody. Two different crystal forms contained the same compact peri-GspD:nanobody heterotetramer. The nanobody contacts peri-GspD mainly via CDR3 and framework residues. The peri-GspD structure reveals three subdomains, with the second and third subdomains exhibiting the KH fold which also occurs in ring-forming proteins of the type 3 secretion system. The first subdomain of GspD is related to domains in phage tail proteins and outer membrane TonB-dependent receptors. A dodecameric peri-GspD model is proposed in which a solvent-accessible β strand of the first subdomain interacts with secreted proteins and/or T2SS partner proteins by β strand complementation.

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KW - PROTEINS

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Crystal Structure of the N-Terminal Domain of the Secretin GspD from ETEC Determined with the Assistance of a Nanobody

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Nota bibliográfica

Financiación

ASJC Scopus subject areas

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