TY - JOUR
T1 - Crystallization of the Aα subunit of protein phosphatase 2A
AU - Skidmore, M. O.
AU - Sawaya, M. R.
AU - Parkin, S.
AU - Rupp, B.
AU - Hope, H.
AU - Everse, S. J.
AU - Walter, G.
PY - 1996
Y1 - 1996
N2 - The Aα subunit of human protein phosphatase 2A forms crystals in space group P21 with cell dimensions a = 104.0, b = 174.9, c = 168.2 Å, and β angle = 90.2°. At cryogenic temperatures, the crystals diffracted to a resolution limit of ~3.0 Å. Based on the unit cell dimensions and a calculated molecular mass of 65,277 Da, the Matthews coefficient suggests eight molecules per asymmetric unit. Two native data sets were collected to a nominal resolution of 3.0 Å and merged to provide a set that is 93% complete, with R(sym) of 9.9%.
AB - The Aα subunit of human protein phosphatase 2A forms crystals in space group P21 with cell dimensions a = 104.0, b = 174.9, c = 168.2 Å, and β angle = 90.2°. At cryogenic temperatures, the crystals diffracted to a resolution limit of ~3.0 Å. Based on the unit cell dimensions and a calculated molecular mass of 65,277 Da, the Matthews coefficient suggests eight molecules per asymmetric unit. Two native data sets were collected to a nominal resolution of 3.0 Å and merged to provide a set that is 93% complete, with R(sym) of 9.9%.
KW - X-ray crystallography
KW - cryocooling
KW - crystallization
KW - protein phosphatase 2A
UR - https://www.scopus.com/pages/publications/0029999748
UR - https://www.scopus.com/pages/publications/0029999748#tab=citedBy
U2 - 10.1002/pro.5560050625
DO - 10.1002/pro.5560050625
M3 - Article
C2 - 8762154
AN - SCOPUS:0029999748
SN - 0961-8368
VL - 5
SP - 1198
EP - 1200
JO - Protein Science
JF - Protein Science
IS - 6
ER -