Detection of Borrelia burgdorferi gene expression during mammalian infection using transcriptional fusions that produce green fluorescent protein

Jennifer C. Miller; Kate von Lackum; Michael E. Woodman; Brian Stevenson

doi:10.1016/j.micpath.2006.04.004

Detection of Borrelia burgdorferi gene expression during mammalian infection using transcriptional fusions that produce green fluorescent protein

Jennifer C. Miller
, Kate von Lackum
, Michael E. Woodman
, Brian Stevenson

Producción científica: Article › revisión exhaustiva

28 Citas (Scopus)

Resumen

A novel, infectious Borrelia burgdorferi that expresses green fluorescent protein (GFP) was developed to examine the utility of this marker protein to label live bacteria during infection processes. Use of a borrelial erpAB promoter to direct gfp transcription supported previous indications that B. burgdorferi expresses erp genes during chronic mammalian infection and during acquisition by feeding ticks. Live bacteria fluoresced and were seen to be located extracellularly in infected mice and within midguts of infected ticks. These results indicate that transcriptional fusions between B. burgdorferi promoters and gfp can be useful tools to examine spirochete gene expression in vivo.

Idioma original	English
Páginas (desde-hasta)	43-47
Número de páginas	5
Publicación	Microbial Pathogenesis
Volumen	41
N.º	1
DOI	https://doi.org/10.1016/j.micpath.2006.04.004
Estado	Published - jul 2006

Nota bibliográfica

Funding Information:
These studies were funded by US National Institutes of Health Grants R01 AI44252 and T32 AI49795. We thank Kelly Babb, Sara Bair, Jerry Bowman, Tomasz Bykowski, Anne Cooley, Sarah Kearns, Natalie Mickelsen and Sean Riley for assistance.

Financiación

These studies were funded by US National Institutes of Health Grants R01 AI44252 and T32 AI49795. We thank Kelly Babb, Sara Bair, Jerry Bowman, Tomasz Bykowski, Anne Cooley, Sarah Kearns, Natalie Mickelsen and Sean Riley for assistance.

Financiadores	Número del financiador
National Institutes of Health (NIH)	R01 AI44252
National Institute of Allergy and Infectious Diseases	T32AI049795

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Microbiology
Infectious Diseases

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10.1016/j.micpath.2006.04.004

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title = "Detection of Borrelia burgdorferi gene expression during mammalian infection using transcriptional fusions that produce green fluorescent protein",

abstract = "A novel, infectious Borrelia burgdorferi that expresses green fluorescent protein (GFP) was developed to examine the utility of this marker protein to label live bacteria during infection processes. Use of a borrelial erpAB promoter to direct gfp transcription supported previous indications that B. burgdorferi expresses erp genes during chronic mammalian infection and during acquisition by feeding ticks. Live bacteria fluoresced and were seen to be located extracellularly in infected mice and within midguts of infected ticks. These results indicate that transcriptional fusions between B. burgdorferi promoters and gfp can be useful tools to examine spirochete gene expression in vivo.",

keywords = "Borrelia burgdorferi, Gene fusion, Lyme disease, Spirochete",

author = "Miller, \{Jennifer C.\} and \{von Lackum\}, Kate and Woodman, \{Michael E.\} and Brian Stevenson",

note = "Funding Information: These studies were funded by US National Institutes of Health Grants R01 AI44252 and T32 AI49795. We thank Kelly Babb, Sara Bair, Jerry Bowman, Tomasz Bykowski, Anne Cooley, Sarah Kearns, Natalie Mickelsen and Sean Riley for assistance.",

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AU - Miller, Jennifer C.

AU - von Lackum, Kate

AU - Woodman, Michael E.

AU - Stevenson, Brian

N1 - Funding Information: These studies were funded by US National Institutes of Health Grants R01 AI44252 and T32 AI49795. We thank Kelly Babb, Sara Bair, Jerry Bowman, Tomasz Bykowski, Anne Cooley, Sarah Kearns, Natalie Mickelsen and Sean Riley for assistance.

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KW - Borrelia burgdorferi

KW - Gene fusion

KW - Lyme disease

KW - Spirochete

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Detection of Borrelia burgdorferi gene expression during mammalian infection using transcriptional fusions that produce green fluorescent protein

Resumen

Nota bibliográfica

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

Acceder al documento

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Huella

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