Resumen
The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 85-88 |
| Número de páginas | 4 |
| Publicación | Analytical Biochemistry |
| Volumen | 418 |
| N.º | 1 |
| DOI | |
| Estado | Published - nov 1 2011 |
Nota bibliográfica
Funding Information:This work was supported by funding from the NIH ( AI52218 ) and the Laura and Edward Kremers Chair in Natural Products Chemistry (J.S.T.).
Financiación
This work was supported by funding from the NIH ( AI52218 ) and the Laura and Edward Kremers Chair in Natural Products Chemistry (J.S.T.).
| Financiadores | Número del financiador |
|---|---|
| National Institutes of Health (NIH) | |
| National Institute of Allergy and Infectious Diseases | R01AI052218 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Huella
Profundice en los temas de investigación de 'Development of a universal glycosyltransferase assay amenable to high-throughput formats'. En conjunto forman una huella única.Citar esto
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