TY - JOUR
T1 - Differential pH sensitivity of tissue superoxide dismutases
AU - Patel, Samir P.
AU - Katyare, Surendra S.
PY - 2006
Y1 - 2006
N2 - Superoxide dismutase (SOD) activities in the human and rat RBCs and rat liver, kidney, brain and heart mitochondria as well as cytosolic fractions were determined by the pyrogallol assay procedure with slight modifications. Measurements were carried out in 0.1 M potassium phosphate buffer pH 8.0 and 9.2 to assess the pH stability of the SODs from various systems. Under these conditions the SODs from different systems including RBCs exhibited differential pH stability i.e. they displayed differential susceptibility at pH 9.2. Even in a given tissue, the mitochondrial and cytosolic SODs were inactivated differentially at pH 9.2. We also observed that the total mitochondrial and cytosolic SOD contents show a tissue-specific pattern. Our results also suggest that measurements carried out at pH 8.0 may give more realistic estimates of SOD activities.
AB - Superoxide dismutase (SOD) activities in the human and rat RBCs and rat liver, kidney, brain and heart mitochondria as well as cytosolic fractions were determined by the pyrogallol assay procedure with slight modifications. Measurements were carried out in 0.1 M potassium phosphate buffer pH 8.0 and 9.2 to assess the pH stability of the SODs from various systems. Under these conditions the SODs from different systems including RBCs exhibited differential pH stability i.e. they displayed differential susceptibility at pH 9.2. Even in a given tissue, the mitochondrial and cytosolic SODs were inactivated differentially at pH 9.2. We also observed that the total mitochondrial and cytosolic SOD contents show a tissue-specific pattern. Our results also suggest that measurements carried out at pH 8.0 may give more realistic estimates of SOD activities.
KW - Cytosolic SOD
KW - Erythrocyte SOD
KW - Mitochondrial SOD
KW - Superoxide dismutase
KW - pH sensitivity
UR - https://www.scopus.com/pages/publications/33644928647
UR - https://www.scopus.com/inward/citedby.url?scp=33644928647&partnerID=8YFLogxK
U2 - 10.1007/BF02912927
DO - 10.1007/BF02912927
M3 - Article
C2 - 23105629
AN - SCOPUS:33644928647
SN - 0970-1915
VL - 21
SP - 129
EP - 133
JO - Indian Journal of Clinical Biochemistry
JF - Indian Journal of Clinical Biochemistry
IS - 2
ER -