Effect of dopamine on NaCl transport in the medullary thick ascending limb of the rat

Jay Grider; Eric Kilpatrick; Cobern Ott; Brian Jackson

doi:10.1016/S0014-2999(97)01564-1

Effect of dopamine on NaCl transport in the medullary thick ascending limb of the rat

Jay Grider
, Eric Kilpatrick
, Cobern Ott
, Brian Jackson

Producción científica: Article › revisión exhaustiva

22 Citas (Scopus)

Resumen

The aim of the present study was to determine whether dopamine affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle. Basolateral dopamine (10^-6 M) significantly inhibited Cl^- reabsorption in the in vitro microperfused rat medullary thick ascending limb by 21 ± 2% (P < 0.01). The response to 10^-6 M dopamine was completely blocked by pretreatment with the dopamine D₁ receptor antagonist R(+)-SCH-23390 (5 x 10^-5 M), and was mimicked by the dopamine D₁ receptor agonist A-77636 (10^-6 M; Δ - 36 ± 2%; P < 0.05). In contrast, basolateral administration of the dopamine D₂ receptor agonist (+)-bromocriptine (10^-6 M) transiently increased Cl^- reabsorption by 49 ± 18% (P < 0.05). Neither the D₁ nor the D₂ agonist affected Cl^- reabsorption when selectively administered to the luminal membrane. These data suggest that the predominant effect of dopamine on the medullary thick ascending limb of the rat is to inhibit the reabsorption of NaCl, a response which is mediated by dopamine D₁ receptors.

Idioma original	English
Páginas (desde-hasta)	281-284
Número de páginas	4
Publicación	European Journal of Pharmacology
Volumen	342
N.º	2-3
DOI	https://doi.org/10.1016/S0014-2999(97)01564-1
Estado	Published - ene 26 1998

Nota bibliográfica

Funding Information:
These studies were supported by a grant-in-aid from the American Heart Association (B.A.J.) and by a NASA EPSCoR grant WKU 522611 (C.E.O.).

Financiación

These studies were supported by a grant-in-aid from the American Heart Association (B.A.J.) and by a NASA EPSCoR grant WKU 522611 (C.E.O.).

Financiadores	Número del financiador
Kentucky NASA EPSCoR	WKU 522611
American Heart Association

ASJC Scopus subject areas

Pharmacology

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10.1016/S0014-2999(97)01564-1

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title = "Effect of dopamine on NaCl transport in the medullary thick ascending limb of the rat",

abstract = "The aim of the present study was to determine whether dopamine affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle. Basolateral dopamine (10-6 M) significantly inhibited Cl- reabsorption in the in vitro microperfused rat medullary thick ascending limb by 21 ± 2\% (P < 0.01). The response to 10-6 M dopamine was completely blocked by pretreatment with the dopamine D1 receptor antagonist R(+)-SCH-23390 (5 x 10-5 M), and was mimicked by the dopamine D1 receptor agonist A-77636 (10-6 M; Δ - 36 ± 2\%; P < 0.05). In contrast, basolateral administration of the dopamine D2 receptor agonist (+)-bromocriptine (10-6 M) transiently increased Cl- reabsorption by 49 ± 18\% (P < 0.05). Neither the D1 nor the D2 agonist affected Cl- reabsorption when selectively administered to the luminal membrane. These data suggest that the predominant effect of dopamine on the medullary thick ascending limb of the rat is to inhibit the reabsorption of NaCl, a response which is mediated by dopamine D1 receptors.",

keywords = "Dopamine, Ion transport, Loop of Henle, Microperfusion, in vitro",

author = "Jay Grider and Eric Kilpatrick and Cobern Ott and Brian Jackson",

note = "Funding Information: These studies were supported by a grant-in-aid from the American Heart Association (B.A.J.) and by a NASA EPSCoR grant WKU 522611 (C.E.O.).",

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AU - Grider, Jay

AU - Kilpatrick, Eric

AU - Ott, Cobern

AU - Jackson, Brian

N1 - Funding Information: These studies were supported by a grant-in-aid from the American Heart Association (B.A.J.) and by a NASA EPSCoR grant WKU 522611 (C.E.O.).

PY - 1998/1/26

Y1 - 1998/1/26

N2 - The aim of the present study was to determine whether dopamine affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle. Basolateral dopamine (10-6 M) significantly inhibited Cl- reabsorption in the in vitro microperfused rat medullary thick ascending limb by 21 ± 2% (P < 0.01). The response to 10-6 M dopamine was completely blocked by pretreatment with the dopamine D1 receptor antagonist R(+)-SCH-23390 (5 x 10-5 M), and was mimicked by the dopamine D1 receptor agonist A-77636 (10-6 M; Δ - 36 ± 2%; P < 0.05). In contrast, basolateral administration of the dopamine D2 receptor agonist (+)-bromocriptine (10-6 M) transiently increased Cl- reabsorption by 49 ± 18% (P < 0.05). Neither the D1 nor the D2 agonist affected Cl- reabsorption when selectively administered to the luminal membrane. These data suggest that the predominant effect of dopamine on the medullary thick ascending limb of the rat is to inhibit the reabsorption of NaCl, a response which is mediated by dopamine D1 receptors.

AB - The aim of the present study was to determine whether dopamine affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle. Basolateral dopamine (10-6 M) significantly inhibited Cl- reabsorption in the in vitro microperfused rat medullary thick ascending limb by 21 ± 2% (P < 0.01). The response to 10-6 M dopamine was completely blocked by pretreatment with the dopamine D1 receptor antagonist R(+)-SCH-23390 (5 x 10-5 M), and was mimicked by the dopamine D1 receptor agonist A-77636 (10-6 M; Δ - 36 ± 2%; P < 0.05). In contrast, basolateral administration of the dopamine D2 receptor agonist (+)-bromocriptine (10-6 M) transiently increased Cl- reabsorption by 49 ± 18% (P < 0.05). Neither the D1 nor the D2 agonist affected Cl- reabsorption when selectively administered to the luminal membrane. These data suggest that the predominant effect of dopamine on the medullary thick ascending limb of the rat is to inhibit the reabsorption of NaCl, a response which is mediated by dopamine D1 receptors.

KW - Dopamine

KW - Ion transport

KW - Loop of Henle

KW - Microperfusion, in vitro

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Effect of dopamine on NaCl transport in the medullary thick ascending limb of the rat

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