Effect of endotoxin on lipid order and motion in erythrocyte membranes

D. Allan Butterfield; Bin Sun; Srithal Bellary; Warwick A. Arden; Kimberly Ward Anderson

doi:10.1016/0925-4439(94)90083-3

Effect of endotoxin on lipid order and motion in erythrocyte membranes

D. Allan Butterfield
, Bin Sun
, Srithal Bellary
, Warwick A. Arden
, Kimberly Ward Anderson

Producción científica: Article › revisión exhaustiva

18 Citas (Scopus)

Resumen

Electron paramagnetic resonance employing a lipid-specific spin label has been used to investigate the molecular effects of endotoxin on the physical state of bilayer lipids in rat erythrocyte membranes. When added at a concentration as low as 40 μg/ml to whole blood (plasma plus leukocytes present), decreased membrane lipid motion was found in subsequently washed and spin-labeled intact erythrocytes (P < 0.02). However, if endotoxin were added to washed, plasma plus leukocyte-free intact erythrocytes, no change in the motion of the spin label was found, suggesting that plasma-soluble substances and/or leukocytes are required to produce the change in the physical state of lipids. The decreased lipid motion found in these studies is discussed with reference to the known decreased deformability of endotoxin-treated red cells and to the pathogenesis of sepsis.

Idioma original	English
Páginas (desde-hasta)	231-234
Número de páginas	4
Publicación	Biochimica et Biophysica Acta - Molecular Basis of Disease
Volumen	1225
N.º	2
DOI	https://doi.org/10.1016/0925-4439(94)90083-3
Estado	Published - ene 11 1994

Nota bibliográfica

Funding Information:
This work was supported in part by grants from NSF \[EHR-9108764\] (D.A.B. and K.W.A.), \[CTS-9157856\] (K.W.A.) and NIH \[AG-10836\] (D.A.B.), \[HL-02918\] (W.A.A.).

Financiación

This work was supported in part by grants from NSF \[EHR-9108764\] (D.A.B. and K.W.A.), \[CTS-9157856\] (K.W.A.) and NIH \[AG-10836\] (D.A.B.), \[HL-02918\] (W.A.A.).

Financiadores	Número del financiador
National Science Foundation (NSF)	EHR-9108764\, CTS-9157856\
National Institutes of Health (NIH)	AG-10836\
National Heart, Lung, and Blood Institute (NHLBI)	K08HL002918

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology

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10.1016/0925-4439(94)90083-3

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@article{db8e2e2420634b82a18210ff9299503d,

title = "Effect of endotoxin on lipid order and motion in erythrocyte membranes",

abstract = "Electron paramagnetic resonance employing a lipid-specific spin label has been used to investigate the molecular effects of endotoxin on the physical state of bilayer lipids in rat erythrocyte membranes. When added at a concentration as low as 40 μg/ml to whole blood (plasma plus leukocytes present), decreased membrane lipid motion was found in subsequently washed and spin-labeled intact erythrocytes (P < 0.02). However, if endotoxin were added to washed, plasma plus leukocyte-free intact erythrocytes, no change in the motion of the spin label was found, suggesting that plasma-soluble substances and/or leukocytes are required to produce the change in the physical state of lipids. The decreased lipid motion found in these studies is discussed with reference to the known decreased deformability of endotoxin-treated red cells and to the pathogenesis of sepsis.",

keywords = "5-doxylstearic acid, Endotoxin, Lipid fluidity, Sepsis, Spin labeling",

author = "Butterfield, \{D. Allan\} and Bin Sun and Srithal Bellary and Arden, \{Warwick A.\} and Anderson, \{Kimberly Ward\}",

note = "Funding Information: This work was supported in part by grants from NSF \textbackslash{}[EHR-9108764\textbackslash{}] (D.A.B. and K.W.A.), \textbackslash{}[CTS-9157856\textbackslash{}] (K.W.A.) and NIH \textbackslash{}[AG-10836\textbackslash{}] (D.A.B.), \textbackslash{}[HL-02918\textbackslash{}] (W.A.A.).",

year = "1994",

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doi = "10.1016/0925-4439(94)90083-3",

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T1 - Effect of endotoxin on lipid order and motion in erythrocyte membranes

AU - Butterfield, D. Allan

AU - Sun, Bin

AU - Bellary, Srithal

AU - Arden, Warwick A.

AU - Anderson, Kimberly Ward

N1 - Funding Information: This work was supported in part by grants from NSF \[EHR-9108764\] (D.A.B. and K.W.A.), \[CTS-9157856\] (K.W.A.) and NIH \[AG-10836\] (D.A.B.), \[HL-02918\] (W.A.A.).

PY - 1994/1/11

Y1 - 1994/1/11

N2 - Electron paramagnetic resonance employing a lipid-specific spin label has been used to investigate the molecular effects of endotoxin on the physical state of bilayer lipids in rat erythrocyte membranes. When added at a concentration as low as 40 μg/ml to whole blood (plasma plus leukocytes present), decreased membrane lipid motion was found in subsequently washed and spin-labeled intact erythrocytes (P < 0.02). However, if endotoxin were added to washed, plasma plus leukocyte-free intact erythrocytes, no change in the motion of the spin label was found, suggesting that plasma-soluble substances and/or leukocytes are required to produce the change in the physical state of lipids. The decreased lipid motion found in these studies is discussed with reference to the known decreased deformability of endotoxin-treated red cells and to the pathogenesis of sepsis.

AB - Electron paramagnetic resonance employing a lipid-specific spin label has been used to investigate the molecular effects of endotoxin on the physical state of bilayer lipids in rat erythrocyte membranes. When added at a concentration as low as 40 μg/ml to whole blood (plasma plus leukocytes present), decreased membrane lipid motion was found in subsequently washed and spin-labeled intact erythrocytes (P < 0.02). However, if endotoxin were added to washed, plasma plus leukocyte-free intact erythrocytes, no change in the motion of the spin label was found, suggesting that plasma-soluble substances and/or leukocytes are required to produce the change in the physical state of lipids. The decreased lipid motion found in these studies is discussed with reference to the known decreased deformability of endotoxin-treated red cells and to the pathogenesis of sepsis.

KW - 5-doxylstearic acid

KW - Endotoxin

KW - Lipid fluidity

KW - Sepsis

KW - Spin labeling

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U2 - 10.1016/0925-4439(94)90083-3

DO - 10.1016/0925-4439(94)90083-3

M3 - Article

C2 - 8280792

AN - SCOPUS:0028144490

SN - 0925-4439

VL - 1225

SP - 231

EP - 234

JO - Biochimica et Biophysica Acta - Molecular Basis of Disease

JF - Biochimica et Biophysica Acta - Molecular Basis of Disease

IS - 2

ER -

Effect of endotoxin on lipid order and motion in erythrocyte membranes

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

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Huella

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