TY - JOUR
T1 - Effect of yohimbine on nerve growth factor mRNA and protein levels in rat hippocampus
AU - Stone, E. A.
AU - Manavalan, J. S.
AU - Basham, D. A.
AU - Bing, G.
PY - 1994/2/14
Y1 - 1994/2/14
N2 - Activation of noradrenergic receptors has been shown to increase expression of nerve growth factor (NGF) gene in brain cells in vitro. The present studies were undertaken to determine if this stimulation was effective in vivo as well. Rats were administered the norepinephrine-releasing drug. yohimbine (YOH), and had their hippocampi assayed for NGF mRNA and protein at various times after the injection. It was found that yohimbine caused a 3-fold increase of NGF mRNA levels at 24 h. Protein levels, however, were unaltered at this time. Thus norepinephrine release in vivo appears to be sufficient for increasing mRNA level but not for translation to protein.
AB - Activation of noradrenergic receptors has been shown to increase expression of nerve growth factor (NGF) gene in brain cells in vitro. The present studies were undertaken to determine if this stimulation was effective in vivo as well. Rats were administered the norepinephrine-releasing drug. yohimbine (YOH), and had their hippocampi assayed for NGF mRNA and protein at various times after the injection. It was found that yohimbine caused a 3-fold increase of NGF mRNA levels at 24 h. Protein levels, however, were unaltered at this time. Thus norepinephrine release in vivo appears to be sufficient for increasing mRNA level but not for translation to protein.
KW - Nerve growth factor mRNA
KW - Nerve growth factor protein
KW - Noradrenergic stimulation
KW - Yohimbine
UR - https://www.scopus.com/pages/publications/0028316527
UR - https://www.scopus.com/pages/publications/0028316527#tab=citedBy
U2 - 10.1016/0304-3940(94)91016-2
DO - 10.1016/0304-3940(94)91016-2
M3 - Article
C2 - 8177507
AN - SCOPUS:0028316527
SN - 0304-3940
VL - 167
SP - 11
EP - 13
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -