Electrical stimulation enhances early palatal wound healing in mice

Camila Lopes Ferreira; Maria Aparecida Neves Jardini; Camilla Magnoni Moretto Nunes; Daniella Vicensotto Bernardo; Renato Corrêa Viana Casarin; Estevão dos Santos Gedraite; Márcio Antônio Mathias; Fei Liu; Gustavo Mendonça; Daniela Baccelli Silveira Mendonça; Mauro Pedrine Santamaria

doi:10.1016/j.archoralbio.2020.105028

Electrical stimulation enhances early palatal wound healing in mice

Camila Lopes Ferreira
, Maria Aparecida Neves Jardini
, Camilla Magnoni Moretto Nunes
, Daniella Vicensotto Bernardo
, Renato Corrêa Viana Casarin
, Estevão dos Santos Gedraite
, Márcio Antônio Mathias
, Fei Liu
, Gustavo Mendonça
, Daniela Baccelli Silveira Mendonça
, Mauro Pedrine Santamaria

Producción científica: Article › revisión exhaustiva

18 Citas (Scopus)

Resumen

Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.

Idioma original	English
Número de artículo	105028
Publicación	Archives of Oral Biology
Volumen	122
DOI	https://doi.org/10.1016/j.archoralbio.2020.105028
Estado	Published - feb 2021

Nota bibliográfica

Publisher Copyright:
© 2020 Elsevier Ltd

Financiación

The authors appreciate the financial support provided by the Research Funding Agency from São Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9 ; CNPq - National Council for Scientific and Technological Development – Brazil for supporting Dr. Mauro P. Santamaria ( Grant #301102/2016-3 ). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES) – Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE). The authors appreciate the financial support provided by the Research Funding Agency from S?o Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9; CNPq - National Council for Scientific and Technological Development ? Brazil for supporting Dr. Mauro P. Santamaria (Grant #301102/2016-3). This study was financed in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior ? Brazil (CAPES) ? Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE).

Financiadores	Número del financiador
CAPES-PDSE
S?o Paulo State
Fundação de Amparo à Pesquisa do Estado de São Paulo	2016/16102-5, 2016/07528-9
Fundação de Amparo à Pesquisa do Estado de São Paulo
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Conselho Nacional de Desenvolvimento Científico e Tecnológico	301102/2016-3
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Universidade Cidade de São Paulo

ASJC Scopus subject areas

Otorhinolaryngology
General Dentistry
Cell Biology

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10.1016/j.archoralbio.2020.105028

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Ferreira, C. L., Neves Jardini, M. A., Moretto Nunes, C. M., Bernardo, D. V., Viana Casarin, R. C., dos Santos Gedraite, E., Mathias, M. A., Liu, F., Mendonça, G., Silveira Mendonça, D. B., & Santamaria, M. P. (2021). Electrical stimulation enhances early palatal wound healing in mice. Archives of Oral Biology, 122, Artículo 105028. https://doi.org/10.1016/j.archoralbio.2020.105028

@article{117a2930c5d94525bb1a9a8329fb9a7b,

title = "Electrical stimulation enhances early palatal wound healing in mice",

abstract = "Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.",

keywords = "Electrical stimulation, Gingival recession, Hard palate, Oral mucosa, Re-epithelialization, Wound healing",

author = "Ferreira, \{Camila Lopes\} and \{Neves Jardini\}, \{Maria Aparecida\} and \{Moretto Nunes\}, \{Camilla Magnoni\} and Bernardo, \{Daniella Vicensotto\} and \{Viana Casarin\}, \{Renato Corr{\^e}a\} and \{dos Santos Gedraite\}, Estev{\~a}o and Mathias, \{M{\'a}rcio Ant{\^o}nio\} and Fei Liu and Gustavo Mendon{\c c}a and \{Silveira Mendon{\c c}a\}, \{Daniela Baccelli\} and Santamaria, \{Mauro Pedrine\}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2021",

month = feb,

doi = "10.1016/j.archoralbio.2020.105028",

language = "English",

volume = "122",

}

TY - JOUR

T1 - Electrical stimulation enhances early palatal wound healing in mice

AU - Ferreira, Camila Lopes

AU - Neves Jardini, Maria Aparecida

AU - Moretto Nunes, Camilla Magnoni

AU - Bernardo, Daniella Vicensotto

AU - Viana Casarin, Renato Corrêa

AU - dos Santos Gedraite, Estevão

AU - Mathias, Márcio Antônio

AU - Liu, Fei

AU - Mendonça, Gustavo

AU - Silveira Mendonça, Daniela Baccelli

AU - Santamaria, Mauro Pedrine

PY - 2021/2

Y1 - 2021/2

N2 - Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.

AB - Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.

KW - Electrical stimulation

KW - Gingival recession

KW - Hard palate

KW - Oral mucosa

KW - Re-epithelialization

KW - Wound healing

UR - https://www.scopus.com/pages/publications/85098602226

UR - https://www.scopus.com/pages/publications/85098602226#tab=citedBy

U2 - 10.1016/j.archoralbio.2020.105028

DO - 10.1016/j.archoralbio.2020.105028

M3 - Article

C2 - 33360374

AN - SCOPUS:85098602226

SN - 0003-9969

VL - 122

JO - Archives of Oral Biology

JF - Archives of Oral Biology

M1 - 105028

ER -

Electrical stimulation enhances early palatal wound healing in mice

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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