Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

Claudio W. Pikielny; Brian C. Rymond; Michael Rosbash

doi:10.1038/324341a0

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

Claudio W. Pikielny
, Brian C. Rymond
, Michael Rosbash

Biology

Producción científica: Article › revisión exhaustiva

185 Citas (Scopus)

Resumen

Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.

Idioma original	English
Páginas (desde-hasta)	341-345
Número de páginas	5
Publicación	Nature
Volumen	324
N.º	6095
DOI	https://doi.org/10.1038/324341a0
Estado	Published - 1986

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Financiadores	Número del financiador
National Institute of General Medical Sciences	R01GM023549

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General

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title = "Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes",

abstract = "Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.",

author = "Pikielny, \{Claudio W.\} and Rymond, \{Brian C.\} and Michael Rosbash",

year = "1986",

doi = "10.1038/324341a0",

language = "English",

volume = "324",

pages = "341--345",

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TY - JOUR

T1 - Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

AU - Pikielny, Claudio W.

AU - Rymond, Brian C.

AU - Rosbash, Michael

PY - 1986

Y1 - 1986

N2 - Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.

AB - Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.

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UR - https://www.scopus.com/pages/publications/0022997930#tab=citedBy

U2 - 10.1038/324341a0

DO - 10.1038/324341a0

M3 - Article

C2 - 3537805

AN - SCOPUS:0022997930

SN - 0028-0836

VL - 324

SP - 341

EP - 345

JO - Nature

JF - Nature

IS - 6095

ER -

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

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