Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Alison Schroer; Gaspard Pardon; Erica Castillo; Cheavar Blair; Beth Pruitt

doi:10.1016/j.pbiomolbio.2018.12.001

Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Alison Schroer
, Gaspard Pardon
, Erica Castillo
, Cheavar Blair
, Beth Pruitt

Producción científica: Review article › revisión exhaustiva

22 Citas (Scopus)

Resumen

The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.

Idioma original	English
Páginas (desde-hasta)	3-15
Número de páginas	13
Publicación	Progress in Biophysics and Molecular Biology
Volumen	144
DOI	https://doi.org/10.1016/j.pbiomolbio.2018.12.001
Estado	Published - jul 2019

Nota bibliográfica

Publisher Copyright:
© 2018

Financiación

This work was supported by the American Heart Association [ AHA 17CSA33590101 ], the National Science Foundation [ NSF CMMI 1662431 ; GRFP], the National Academies of Sciences, Engineering, and Medicine [Ford Foundation Fellowship] and the National Institutes of Health [ NIH 1R21HL13099301 ; TIMBS T32 ] and SNSF Early Postdoc.Mobility fellowship grant: [ P2SKP2 _164954 ]. This work was supported by the American Heart Association [AHA 17CSA33590101], the National Science Foundation [NSF CMMI 1662431; GRFP], the National Academies of Sciences, Engineering, and Medicine [Ford Foundation Fellowship] and the National Institutes of Health [NIH 1R21HL13099301; TIMBS T32] and SNSF Early Postdoc.Mobility fellowship grant: [P2SKP2_164954].

Financiadores	Número del financiador
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	CMMI 1662431
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China
National Institutes of Health (NIH)	TIMBS T32
National Institutes of Health (NIH)
Ford Foundation
National Heart, Lung, and Blood Institute Family Blood Pressure Program	R21HL130993
National Heart, Lung, and Blood Institute Family Blood Pressure Program
American the American Heart Association	17CSA33590101
American the American Heart Association
National Academies of Sciences, Engineering, and Medicine
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung	P2SKP2 _164954
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Biophysics
Molecular Biology

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title = "Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment",

abstract = "The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.",

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AU - Pardon, Gaspard

AU - Castillo, Erica

AU - Blair, Cheavar

AU - Pruitt, Beth

PY - 2019/7

Y1 - 2019/7

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KW - Drug discovery

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KW - Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs)

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Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Resumen

Nota bibliográfica

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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Huella

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