Engineering of a multifunctional hemicellulase

Zhanmin Fan; Joshua R. Werkman; Ling Yuan

doi:10.1007/s10529-009-9926-3

Engineering of a multifunctional hemicellulase

Zhanmin Fan
, Joshua R. Werkman
, Ling Yuan

Producción científica: Article › revisión exhaustiva

42 Citas (Scopus)

Resumen

To engineer a multifunctional xylan-degrading enzyme, a chimera was created by fusing the xylanase domain of the Clostridium thermocellum xylanase (xynZ) and a dual functional arabinofuranosidase/xylosidase (DeAFc; from a compost starter mixture) through a flexible peptide linker. The xylanase domain of xynZ possesses previously unreported endoglucanase activity. The chimera, possessing the activities of xylanase, endoglucanase, arabinofuranosidase and xylosidase, was expressed in E. coli and purified. The chimera closely resembled the parental enzymes in pH, temperature optima and kinetics, and was more active than the parental enzyme mixture in the hydrolysis of natural xylans and corn stover.

Idioma original	English
Páginas (desde-hasta)	751-757
Número de páginas	7
Publicación	Biotechnology Letters
Volumen	31
N.º	5
DOI	https://doi.org/10.1007/s10529-009-9926-3
Estado	Published - may 2009

Nota bibliográfica

Funding Information:
Acknowledgements We thank Drs. K. Wagschal and C. Lee of the U.S. Department of Agriculture for providing the DeAFc gene, technical support and helpful discussions. We also thank Dr. I. Maiti of the University of Kentucky for providing the Xyln gene and Dr. M. Montross for supplying the corn stover substrates. This work is supported by a grant from the U.S. Department of Agriculture to L.Y. (2006-35504-17413).

Financiación

Acknowledgements We thank Drs. K. Wagschal and C. Lee of the U.S. Department of Agriculture for providing the DeAFc gene, technical support and helpful discussions. We also thank Dr. I. Maiti of the University of Kentucky for providing the Xyln gene and Dr. M. Montross for supplying the corn stover substrates. This work is supported by a grant from the U.S. Department of Agriculture to L.Y. (2006-35504-17413).

Financiadores	Número del financiador
U.S. Department of Agriculture	2006-35504-17413

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Acceder al documento

10.1007/s10529-009-9926-3

Otros archivos y enlaces

Citar esto

@article{8c6f4a47ea064dda8f483e68278b78a1,

title = "Engineering of a multifunctional hemicellulase",

abstract = "To engineer a multifunctional xylan-degrading enzyme, a chimera was created by fusing the xylanase domain of the Clostridium thermocellum xylanase (xynZ) and a dual functional arabinofuranosidase/xylosidase (DeAFc; from a compost starter mixture) through a flexible peptide linker. The xylanase domain of xynZ possesses previously unreported endoglucanase activity. The chimera, possessing the activities of xylanase, endoglucanase, arabinofuranosidase and xylosidase, was expressed in E. coli and purified. The chimera closely resembled the parental enzymes in pH, temperature optima and kinetics, and was more active than the parental enzyme mixture in the hydrolysis of natural xylans and corn stover.",

keywords = "Arabinoxylans, Biomass conversion, Enzyme engineering, Hemicellulases, Multifunctional enzymes",

author = "Zhanmin Fan and Werkman, \{Joshua R.\} and Ling Yuan",

note = "Funding Information: Acknowledgements We thank Drs. K. Wagschal and C. Lee of the U.S. Department of Agriculture for providing the DeAFc gene, technical support and helpful discussions. We also thank Dr. I. Maiti of the University of Kentucky for providing the Xyln gene and Dr. M. Montross for supplying the corn stover substrates. This work is supported by a grant from the U.S. Department of Agriculture to L.Y. (2006-35504-17413).",

year = "2009",

month = may,

doi = "10.1007/s10529-009-9926-3",

language = "English",

volume = "31",

pages = "751--757",

number = "5",

}

TY - JOUR

T1 - Engineering of a multifunctional hemicellulase

AU - Fan, Zhanmin

AU - Werkman, Joshua R.

AU - Yuan, Ling

N1 - Funding Information: Acknowledgements We thank Drs. K. Wagschal and C. Lee of the U.S. Department of Agriculture for providing the DeAFc gene, technical support and helpful discussions. We also thank Dr. I. Maiti of the University of Kentucky for providing the Xyln gene and Dr. M. Montross for supplying the corn stover substrates. This work is supported by a grant from the U.S. Department of Agriculture to L.Y. (2006-35504-17413).

PY - 2009/5

Y1 - 2009/5

N2 - To engineer a multifunctional xylan-degrading enzyme, a chimera was created by fusing the xylanase domain of the Clostridium thermocellum xylanase (xynZ) and a dual functional arabinofuranosidase/xylosidase (DeAFc; from a compost starter mixture) through a flexible peptide linker. The xylanase domain of xynZ possesses previously unreported endoglucanase activity. The chimera, possessing the activities of xylanase, endoglucanase, arabinofuranosidase and xylosidase, was expressed in E. coli and purified. The chimera closely resembled the parental enzymes in pH, temperature optima and kinetics, and was more active than the parental enzyme mixture in the hydrolysis of natural xylans and corn stover.

AB - To engineer a multifunctional xylan-degrading enzyme, a chimera was created by fusing the xylanase domain of the Clostridium thermocellum xylanase (xynZ) and a dual functional arabinofuranosidase/xylosidase (DeAFc; from a compost starter mixture) through a flexible peptide linker. The xylanase domain of xynZ possesses previously unreported endoglucanase activity. The chimera, possessing the activities of xylanase, endoglucanase, arabinofuranosidase and xylosidase, was expressed in E. coli and purified. The chimera closely resembled the parental enzymes in pH, temperature optima and kinetics, and was more active than the parental enzyme mixture in the hydrolysis of natural xylans and corn stover.

KW - Arabinoxylans

KW - Biomass conversion

KW - Enzyme engineering

KW - Hemicellulases

KW - Multifunctional enzymes

UR - https://www.scopus.com/pages/publications/65249122405

UR - https://www.scopus.com/pages/publications/65249122405#tab=citedBy

U2 - 10.1007/s10529-009-9926-3

DO - 10.1007/s10529-009-9926-3

M3 - Article

C2 - 19169889

AN - SCOPUS:65249122405

SN - 0141-5492

VL - 31

SP - 751

EP - 757

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 5

ER -

Engineering of a multifunctional hemicellulase

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto