Enzyme-mediated bioorthogonal technologies: catalysts, chemoselective reactions and recent methyltransferase applications

Elnaz Jalali; Jon S. Thorson

doi:10.1016/j.copbio.2021.02.010

Enzyme-mediated bioorthogonal technologies: catalysts, chemoselective reactions and recent methyltransferase applications

Elnaz Jalali
, Jon S. Thorson

Producción científica: Review article › revisión exhaustiva

17 Citas (Scopus)

Resumen

Transferases have emerged as among the best catalysts for enzyme-mediated bioorthogonal functional group installation to advance innovative in vitro, cell-based and in vivo chemical biology applications. This review introduces the key considerations for selecting enzyme catalysts and chemoselective reactions most amenable to bioorthogonal platform development and highlights relevant key technology development and applications for one ubiquitous transferase subclass — methyltransferases (MTs). Within this context, recent advances in MT-enabled bioorthogonal labeling/conjugation relevant to DNA, RNA, protein, and natural products (i.e. complex small molecule metabolites) are highlighted.

Idioma original	English
Páginas (desde-hasta)	290-298
Número de páginas	9
Publicación	Current Opinion in Biotechnology
Volumen	69
DOI	https://doi.org/10.1016/j.copbio.2021.02.010
Estado	Published - jun 2021

Nota bibliográfica

Publisher Copyright:
© 2021 Elsevier Ltd

Financiación

We gratefully acknowledge the support of R37 AI52218, R01 GM115261, the Center of Biomedical Research Excellence (COBRE) in Pharmaceutical Research and Innovation (CPRI, N.I.H.P20 GM130456), the University of Kentucky College of Pharmacy and the National Center for Advancing Translational Sciences (UL1TR000117 and UL1TR001998). We gratefully acknowledge the support of R37 AI52218 , R01 GM115261 , the Center of Biomedical Research Excellence (COBRE) in Pharmaceutical Research and Innovation ( CPRI , N.I.H. P20 GM130456 ), the U niversity of Kentucky College of Pharmacy and the National Center for Advancing Translational Sciences ( UL1TR000117 and UL1TR001998 ).

Financiadores	Número del financiador
Center of Biomedical Research Excellence	N.I.H.P20 GM130456
N.I.H.	P20 GM130456
Center for Pharmaceutical Research and Innovation
University of Kentucky College of Pharmacy
National Center for Advancing Translational Sciences (NCATS)	UL1TR001998, UL1TR000117
Central Power Research Institute

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering

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10.1016/j.copbio.2021.02.010

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abstract = "Transferases have emerged as among the best catalysts for enzyme-mediated bioorthogonal functional group installation to advance innovative in vitro, cell-based and in vivo chemical biology applications. This review introduces the key considerations for selecting enzyme catalysts and chemoselective reactions most amenable to bioorthogonal platform development and highlights relevant key technology development and applications for one ubiquitous transferase subclass — methyltransferases (MTs). Within this context, recent advances in MT-enabled bioorthogonal labeling/conjugation relevant to DNA, RNA, protein, and natural products (i.e. complex small molecule metabolites) are highlighted.",

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AU - Thorson, Jon S.

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AB - Transferases have emerged as among the best catalysts for enzyme-mediated bioorthogonal functional group installation to advance innovative in vitro, cell-based and in vivo chemical biology applications. This review introduces the key considerations for selecting enzyme catalysts and chemoselective reactions most amenable to bioorthogonal platform development and highlights relevant key technology development and applications for one ubiquitous transferase subclass — methyltransferases (MTs). Within this context, recent advances in MT-enabled bioorthogonal labeling/conjugation relevant to DNA, RNA, protein, and natural products (i.e. complex small molecule metabolites) are highlighted.

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ER -

Enzyme-mediated bioorthogonal technologies: catalysts, chemoselective reactions and recent methyltransferase applications

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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