ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide

Xianglin Shi; N. S. Dalal

doi:10.3109/10715769009145929

ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide

Xianglin Shi, N. S. Dalal

Toxicology and Cancer Biology

Producción científica: Article › revisión exhaustiva

38 Citas (Scopus)

Resumen

Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H₂O₂) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H₂O₂ to generate the OH radicals. Reaction between H₂O₂ and a Cr(V)-glutathione complex. and between H₂O₂ and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H₂O₂, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity.

Idioma original	English
Páginas (desde-hasta)	17-26
Número de páginas	10
Publicación	Free Radical Research
Volumen	10
N.º	1-2
DOI	https://doi.org/10.3109/10715769009145929
Estado	Published - 1990

Nota bibliográfica

Funding Information:
This research has been supported by the Department of the Interior’s Mineral program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant

Financiación

This research has been supported by the Department of the Interior’s Mineral program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant

Financiadores	Número del financiador
Bureau of Mines
U.S. Department of the Interior

ASJC Scopus subject areas

Biochemistry

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@article{93739839703c4b26a0ca0158dfadd1cc,

title = "ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide",

abstract = "Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H2O2) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H2O2 to generate the OH radicals. Reaction between H2O2 and a Cr(V)-glutathione complex. and between H2O2 and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H2O2, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity.",

keywords = "Cr(V), Cr(VI) reduction, ESR, Free radicals, Hydrogen peroxide, Spin trapping",

author = "Xianglin Shi and Dalal, \{N. S.\}",

note = "Funding Information: This research has been supported by the Department of the Interior{\textquoteright}s Mineral program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant",

year = "1990",

doi = "10.3109/10715769009145929",

language = "English",

volume = "10",

pages = "17--26",

number = "1-2",

}

TY - JOUR

T1 - ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide

AU - Shi, Xianglin

AU - Dalal, N. S.

N1 - Funding Information: This research has been supported by the Department of the Interior’s Mineral program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant

PY - 1990

Y1 - 1990

N2 - Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H2O2) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H2O2 to generate the OH radicals. Reaction between H2O2 and a Cr(V)-glutathione complex. and between H2O2 and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H2O2, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity.

AB - Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H2O2) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H2O2 to generate the OH radicals. Reaction between H2O2 and a Cr(V)-glutathione complex. and between H2O2 and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H2O2, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity.

KW - Cr(V)

KW - Cr(VI) reduction

KW - ESR

KW - Free radicals

KW - Hydrogen peroxide

KW - Spin trapping

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U2 - 10.3109/10715769009145929

DO - 10.3109/10715769009145929

M3 - Article

C2 - 2165982

AN - SCOPUS:0025653302

SN - 1071-5762

VL - 10

SP - 17

EP - 26

JO - Free Radical Research

JF - Free Radical Research

IS - 1-2

ER -

ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide

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Financiación

ASJC Scopus subject areas

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