Expressed sequence tags reveal Proctotrupomorpha (minus Chalcidoidea) as sister to Aculeata (Hymenoptera: Insecta)

Barbara J. Sharanowski; Barbara Robbertse; John Walker; S. Randal Voss; Ryan Yoder; Joseph Spatafora; Michael J. Sharkey

doi:10.1016/j.ympev.2010.07.006

Expressed sequence tags reveal Proctotrupomorpha (minus Chalcidoidea) as sister to Aculeata (Hymenoptera: Insecta)

Barbara J. Sharanowski
, Barbara Robbertse
, John Walker
, S. Randal Voss
, Ryan Yoder
, Joseph Spatafora
, Michael J. Sharkey

Neuroscience

Producción científica: Article › revisión exhaustiva

27 Citas (Scopus)

Resumen

Hymenoptera is one of the most diverse groups of animals on the planet and have vital importance for ecosystem function as pollinators and parasitoids. Higher-level relationships among Hymenoptera have been notoriously difficult to resolve with both morphological and traditional molecular approaches. Here we examined the utility of expressed sequence tags for resolving relationships among hymenopteran superfamilies. Transcripts were assembled for 6 disparate Hymenopteran taxa with additional sequences added from public databases for a final dataset of 24 genes for 16 taxa and over 10. kb of sequence data. The concatenated dataset recovered a robust and well-supported topology demonstrating the monophyly of Holometabola, Hymenoptera, Apocrita, Aculeata, Ichneumonoidea, and a sister relationship between the two most closely related proctotrupomorphs in the dataset (Cynipoidea. +. Proctotrupoidea). The data strongly supported a sister relationship between Aculeata and Proctotrupomorpha, contrary to previously proposed hypotheses. Additionally there was strong evidence indicating Ichneumonoidea as sister to Aculeata. +. Proctotrupomorpha. These relationships were robust to missing data, nucleotide composition biases, low taxonomic sampling, and conflicting signal across gene trees. There was also strong evidence indicating that Chalcidoidea is not contained within Proctotrupomorpha.

Idioma original	English
Páginas (desde-hasta)	101-112
Número de páginas	12
Publicación	Molecular Phylogenetics and Evolution
Volumen	57
N.º	1
DOI	https://doi.org/10.1016/j.ympev.2010.07.006
Estado	Published - oct 2010

Nota bibliográfica

Funding Information:
We gratefully thank Chris Schardl, Eric Chapman, Dicky Yu, Andy Deans, Matt Yoder, and David Wiesrock, and two anonymous reviewers for insightful and thought-inspiring comments that greatly improved the paper. We extend sincere gratitude to Catherin Linnen, Bruce Webb, and Kimberly Ferrero for providing colony specimens. A special thanks to Matt Yoder for writing the perl script for the gene jackknife analysis. We acknowledge the Schisto Genome Network for creating the seqs2dbEST Perl script. We extend our appreciation to Brian Wiegmann, Marc Johnson, and Mark Miller for access to computational resources and software. We also thank Andy Boring, Ray Fisher, Kacie Johansen, Adam Kesheimer, Dicky Yu, and Terry Sharanowski for support with extensive bench work. Additionally, collaborations for this project were made possible through an Assembling the Tree of Life (AToL) symposium organized and supported by NSF. This project was funded through NSF Grant EF-0337220 and the lead author (B.J. Sharanowski) was supported by the Kentucky Opportunity Fellowship provided by the University of Kentucky during part of this project. Appendix A

Financiación

We gratefully thank Chris Schardl, Eric Chapman, Dicky Yu, Andy Deans, Matt Yoder, and David Wiesrock, and two anonymous reviewers for insightful and thought-inspiring comments that greatly improved the paper. We extend sincere gratitude to Catherin Linnen, Bruce Webb, and Kimberly Ferrero for providing colony specimens. A special thanks to Matt Yoder for writing the perl script for the gene jackknife analysis. We acknowledge the Schisto Genome Network for creating the seqs2dbEST Perl script. We extend our appreciation to Brian Wiegmann, Marc Johnson, and Mark Miller for access to computational resources and software. We also thank Andy Boring, Ray Fisher, Kacie Johansen, Adam Kesheimer, Dicky Yu, and Terry Sharanowski for support with extensive bench work. Additionally, collaborations for this project were made possible through an Assembling the Tree of Life (AToL) symposium organized and supported by NSF. This project was funded through NSF Grant EF-0337220 and the lead author (B.J. Sharanowski) was supported by the Kentucky Opportunity Fellowship provided by the University of Kentucky during part of this project. Appendix A

Financiadores	Número del financiador
National Center for Research Resources	R24RR016344
University of Kentucky
National Science Foundation (NSF)	EF-0337220

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics

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10.1016/j.ympev.2010.07.006

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title = "Expressed sequence tags reveal Proctotrupomorpha (minus Chalcidoidea) as sister to Aculeata (Hymenoptera: Insecta)",

abstract = "Hymenoptera is one of the most diverse groups of animals on the planet and have vital importance for ecosystem function as pollinators and parasitoids. Higher-level relationships among Hymenoptera have been notoriously difficult to resolve with both morphological and traditional molecular approaches. Here we examined the utility of expressed sequence tags for resolving relationships among hymenopteran superfamilies. Transcripts were assembled for 6 disparate Hymenopteran taxa with additional sequences added from public databases for a final dataset of 24 genes for 16 taxa and over 10. kb of sequence data. The concatenated dataset recovered a robust and well-supported topology demonstrating the monophyly of Holometabola, Hymenoptera, Apocrita, Aculeata, Ichneumonoidea, and a sister relationship between the two most closely related proctotrupomorphs in the dataset (Cynipoidea. +. Proctotrupoidea). The data strongly supported a sister relationship between Aculeata and Proctotrupomorpha, contrary to previously proposed hypotheses. Additionally there was strong evidence indicating Ichneumonoidea as sister to Aculeata. +. Proctotrupomorpha. These relationships were robust to missing data, nucleotide composition biases, low taxonomic sampling, and conflicting signal across gene trees. There was also strong evidence indicating that Chalcidoidea is not contained within Proctotrupomorpha.",

keywords = "Expressed sequence tags (ESTs), Filtered supernetworks, Gene tree discordance, Hymenoptera, Phylogenomics",

author = "Sharanowski, \{Barbara J.\} and Barbara Robbertse and John Walker and Voss, \{S. Randal\} and Ryan Yoder and Joseph Spatafora and Sharkey, \{Michael J.\}",

note = "Funding Information: We gratefully thank Chris Schardl, Eric Chapman, Dicky Yu, Andy Deans, Matt Yoder, and David Wiesrock, and two anonymous reviewers for insightful and thought-inspiring comments that greatly improved the paper. We extend sincere gratitude to Catherin Linnen, Bruce Webb, and Kimberly Ferrero for providing colony specimens. A special thanks to Matt Yoder for writing the perl script for the gene jackknife analysis. We acknowledge the Schisto Genome Network for creating the seqs2dbEST Perl script. We extend our appreciation to Brian Wiegmann, Marc Johnson, and Mark Miller for access to computational resources and software. We also thank Andy Boring, Ray Fisher, Kacie Johansen, Adam Kesheimer, Dicky Yu, and Terry Sharanowski for support with extensive bench work. Additionally, collaborations for this project were made possible through an Assembling the Tree of Life (AToL) symposium organized and supported by NSF. This project was funded through NSF Grant EF-0337220 and the lead author (B.J. Sharanowski) was supported by the Kentucky Opportunity Fellowship provided by the University of Kentucky during part of this project. Appendix A ",

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doi = "10.1016/j.ympev.2010.07.006",

language = "English",

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AU - Robbertse, Barbara

AU - Walker, John

AU - Voss, S. Randal

AU - Yoder, Ryan

AU - Spatafora, Joseph

AU - Sharkey, Michael J.

N1 - Funding Information: We gratefully thank Chris Schardl, Eric Chapman, Dicky Yu, Andy Deans, Matt Yoder, and David Wiesrock, and two anonymous reviewers for insightful and thought-inspiring comments that greatly improved the paper. We extend sincere gratitude to Catherin Linnen, Bruce Webb, and Kimberly Ferrero for providing colony specimens. A special thanks to Matt Yoder for writing the perl script for the gene jackknife analysis. We acknowledge the Schisto Genome Network for creating the seqs2dbEST Perl script. We extend our appreciation to Brian Wiegmann, Marc Johnson, and Mark Miller for access to computational resources and software. We also thank Andy Boring, Ray Fisher, Kacie Johansen, Adam Kesheimer, Dicky Yu, and Terry Sharanowski for support with extensive bench work. Additionally, collaborations for this project were made possible through an Assembling the Tree of Life (AToL) symposium organized and supported by NSF. This project was funded through NSF Grant EF-0337220 and the lead author (B.J. Sharanowski) was supported by the Kentucky Opportunity Fellowship provided by the University of Kentucky during part of this project. Appendix A

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N2 - Hymenoptera is one of the most diverse groups of animals on the planet and have vital importance for ecosystem function as pollinators and parasitoids. Higher-level relationships among Hymenoptera have been notoriously difficult to resolve with both morphological and traditional molecular approaches. Here we examined the utility of expressed sequence tags for resolving relationships among hymenopteran superfamilies. Transcripts were assembled for 6 disparate Hymenopteran taxa with additional sequences added from public databases for a final dataset of 24 genes for 16 taxa and over 10. kb of sequence data. The concatenated dataset recovered a robust and well-supported topology demonstrating the monophyly of Holometabola, Hymenoptera, Apocrita, Aculeata, Ichneumonoidea, and a sister relationship between the two most closely related proctotrupomorphs in the dataset (Cynipoidea. +. Proctotrupoidea). The data strongly supported a sister relationship between Aculeata and Proctotrupomorpha, contrary to previously proposed hypotheses. Additionally there was strong evidence indicating Ichneumonoidea as sister to Aculeata. +. Proctotrupomorpha. These relationships were robust to missing data, nucleotide composition biases, low taxonomic sampling, and conflicting signal across gene trees. There was also strong evidence indicating that Chalcidoidea is not contained within Proctotrupomorpha.

AB - Hymenoptera is one of the most diverse groups of animals on the planet and have vital importance for ecosystem function as pollinators and parasitoids. Higher-level relationships among Hymenoptera have been notoriously difficult to resolve with both morphological and traditional molecular approaches. Here we examined the utility of expressed sequence tags for resolving relationships among hymenopteran superfamilies. Transcripts were assembled for 6 disparate Hymenopteran taxa with additional sequences added from public databases for a final dataset of 24 genes for 16 taxa and over 10. kb of sequence data. The concatenated dataset recovered a robust and well-supported topology demonstrating the monophyly of Holometabola, Hymenoptera, Apocrita, Aculeata, Ichneumonoidea, and a sister relationship between the two most closely related proctotrupomorphs in the dataset (Cynipoidea. +. Proctotrupoidea). The data strongly supported a sister relationship between Aculeata and Proctotrupomorpha, contrary to previously proposed hypotheses. Additionally there was strong evidence indicating Ichneumonoidea as sister to Aculeata. +. Proctotrupomorpha. These relationships were robust to missing data, nucleotide composition biases, low taxonomic sampling, and conflicting signal across gene trees. There was also strong evidence indicating that Chalcidoidea is not contained within Proctotrupomorpha.

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KW - Filtered supernetworks

KW - Gene tree discordance

KW - Hymenoptera

KW - Phylogenomics

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Expressed sequence tags reveal Proctotrupomorpha (minus Chalcidoidea) as sister to Aculeata (Hymenoptera: Insecta)

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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