Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells

Hiroaki Kusanagi; Akiko Hida; Kohtoku Satoh; Masaru Echizenya; Tetsuo Shimizu; Julie S. Pendergast; Shin Yamazaki; Kazuo Mishima

doi:10.1016/j.neures.2008.01.012

Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells

Hiroaki Kusanagi
, Akiko Hida
, Kohtoku Satoh
, Masaru Echizenya
, Tetsuo Shimizu
, Julie S. Pendergast
, Shin Yamazaki
, Kazuo Mishima

Biology

Producción científica: Article › revisión exhaustiva

90 Citas (Scopus)

Resumen

The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.

Idioma original	English
Páginas (desde-hasta)	136-142
Número de páginas	7
Publicación	Neuroscience Research
Volumen	61
N.º	2
DOI	https://doi.org/10.1016/j.neures.2008.01.012
Estado	Published - jun 2008

Nota bibliográfica

Funding Information:
This study was supported by Special Coordination Funds of the Ministry of Education, Culture, Sports, and Technology of Japan, and by a Grant-in-Aid for Cooperative Research from the Ministry of Health, Labour, and Welfare of Japan.

Financiación

This study was supported by Special Coordination Funds of the Ministry of Education, Culture, Sports, and Technology of Japan, and by a Grant-in-Aid for Cooperative Research from the Ministry of Health, Labour, and Welfare of Japan.

Financiadores
Ministry of Education, Culture, Sports, and Technology of Japan
Ministry of Health, Labour and Welfare

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neures.2008.01.012

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title = "Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells",

abstract = "The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.",

keywords = "Circadian, Clock genes, Expression profiles, Human peripheral blood mononuclear cells (PBMCs), Period (Per)",

author = "Hiroaki Kusanagi and Akiko Hida and Kohtoku Satoh and Masaru Echizenya and Tetsuo Shimizu and Pendergast, \{Julie S.\} and Shin Yamazaki and Kazuo Mishima",

note = "Funding Information: This study was supported by Special Coordination Funds of the Ministry of Education, Culture, Sports, and Technology of Japan, and by a Grant-in-Aid for Cooperative Research from the Ministry of Health, Labour, and Welfare of Japan.",

year = "2008",

month = jun,

doi = "10.1016/j.neures.2008.01.012",

language = "English",

volume = "61",

pages = "136--142",

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TY - JOUR

T1 - Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells

AU - Kusanagi, Hiroaki

AU - Hida, Akiko

AU - Satoh, Kohtoku

AU - Echizenya, Masaru

AU - Shimizu, Tetsuo

AU - Pendergast, Julie S.

AU - Yamazaki, Shin

AU - Mishima, Kazuo

N1 - Funding Information: This study was supported by Special Coordination Funds of the Ministry of Education, Culture, Sports, and Technology of Japan, and by a Grant-in-Aid for Cooperative Research from the Ministry of Health, Labour, and Welfare of Japan.

PY - 2008/6

Y1 - 2008/6

N2 - The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.

AB - The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.

KW - Circadian

KW - Clock genes

KW - Expression profiles

KW - Human peripheral blood mononuclear cells (PBMCs)

KW - Period (Per)

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UR - https://www.scopus.com/pages/publications/42749086848#tab=citedBy

U2 - 10.1016/j.neures.2008.01.012

DO - 10.1016/j.neures.2008.01.012

M3 - Article

C2 - 18336944

AN - SCOPUS:42749086848

SN - 0168-0102

VL - 61

SP - 136

EP - 142

JO - Neuroscience Research

JF - Neuroscience Research

IS - 2

ER -

Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells

Resumen

Nota bibliográfica

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

Acceder al documento

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Huella

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