Extracellular vesicles released after cranial radiation: An insight into an early mechanism of brain injury

Suriyan Sukati; Jenni Ho; Luksana Chaiswing; Pradoldej Sompol; Harshul Pandit; Wendy Wei; Tadahide Izumi; Quan Chen; Heidi Weiss; Teresa Noel; Subbarao Bondada; D. Allan Butterfield; Daret K. St. Clair

doi:10.1016/j.brainres.2022.147840

Extracellular vesicles released after cranial radiation: An insight into an early mechanism of brain injury

Suriyan Sukati
, Jenni Ho
, Luksana Chaiswing
, Pradoldej Sompol
, Harshul Pandit
, Wendy Wei
, Tadahide Izumi
, Quan Chen
, Heidi Weiss
, Teresa Noel
, Subbarao Bondada
, D. Allan Butterfield
, Daret K. St. Clair

Producción científica: Article › revisión exhaustiva

15 Citas (Scopus)

Resumen

Cranial radiation is important for treating both primary brain tumors and brain metastases. A potential delayed side effect of cranial radiation is neurocognitive function decline. Early detection of CNS injury might prevent further neuronal damage. Extracellular vesicles (EVs) have emerged as a potential diagnostic tool because of their unique membranous characteristics and cargos. We investigated whether EVs can be an early indicator of CNS injury by giving C57BJ/6 mice 10 Gy cranial IR. EVs were isolated from sera to quantify: 1) number of EVs using nanoparticle tracking analysis (NTA); 2) Glial fibrillary acidic protein (GFAP), an astrocyte marker; and 3) protein-bound 4-hydroxy-2-nonenal (HNE) adducts, an oxidative damage marker. Brain tissues were prepared for immunohistochemistry staining and protein immunoblotting. The results demonstrate: 1) increased GFAP levels (p < 0.05) in EVs, but not brain tissue, in the IR group; and 2) increased HNE-bound protein adduction levels (p < 0.05). The results support using EVs as an early indicator of cancer therapy-induced neuronal injury.

Idioma original	English
Número de artículo	147840
Publicación	Brain Research
Volumen	1782
DOI	https://doi.org/10.1016/j.brainres.2022.147840
Estado	Published - may 1 2022

Nota bibliográfica

Publisher Copyright:
© 2022 The Authors

Financiación

This work was supported by the National Institutes of Health (5R01CA217934-04), National Institute of Environmental Health Sciences (T32ES07266), the National Cancer Institute Cancer Center Support Grant (P30 CA177558), the National Institue of General Medical Sciences (P20 P20 GM121327), and the University of Kentucky Center for Clinical and Translational Science (NIH-UL1TR001998). The authors would like to acknowledge the Biospecimen Procurement and Translational Pathology Shared Resource Facility (Dana Napier and Dr. Therese Bocklage), the Redox Metabolism Shared Resource Facility (Michael Alstott), and the Biostatistics and Bioinformatics Shared Resource Facility at the Markey Cancer Center. This work was supported by the National Institutes of Health (5R01CA217934-04), National Institute of Environmental Health Sciences (T32ES07266), the National Cancer Institute Cancer Center Support Grant (P30 CA177558), the National Institue of General Medical Sciences (P20 P20 GM121327), and the University of Kentucky Center for Clinical and Translational Science (NIH-UL1TR001998).

Financiadores	Número del financiador
National Institutes of Health (NIH)
The Markey Biostatistics and Bioinformatics Shared Resource Facility
Markey Cancer Center Redox and Metabolism Shared Resource Facility
University of Kentucky, Center for Clinical and Translational Science	NIH-UL1TR001998
National Institute of General Medical Sciences DP2GM119177 Sophie Dumont National Institute of General Medical Sciences	P20GM121327
National Childhood Cancer Registry – National Cancer Institute	R01CA217934, P30CA177558
National Institutes of Health/National Institute of Environmental Health Sciences	T32ES007266
National Center for Advancing Translational Sciences (NCATS)	UL1TR001998

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Extracellular vesicles released after cranial radiation: An insight into an early mechanism of brain injury",

abstract = "Cranial radiation is important for treating both primary brain tumors and brain metastases. A potential delayed side effect of cranial radiation is neurocognitive function decline. Early detection of CNS injury might prevent further neuronal damage. Extracellular vesicles (EVs) have emerged as a potential diagnostic tool because of their unique membranous characteristics and cargos. We investigated whether EVs can be an early indicator of CNS injury by giving C57BJ/6 mice 10 Gy cranial IR. EVs were isolated from sera to quantify: 1) number of EVs using nanoparticle tracking analysis (NTA); 2) Glial fibrillary acidic protein (GFAP), an astrocyte marker; and 3) protein-bound 4-hydroxy-2-nonenal (HNE) adducts, an oxidative damage marker. Brain tissues were prepared for immunohistochemistry staining and protein immunoblotting. The results demonstrate: 1) increased GFAP levels (p < 0.05) in EVs, but not brain tissue, in the IR group; and 2) increased HNE-bound protein adduction levels (p < 0.05). The results support using EVs as an early indicator of cancer therapy-induced neuronal injury.",

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AU - Izumi, Tadahide

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Extracellular vesicles released after cranial radiation: An insight into an early mechanism of brain injury

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Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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